New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3349143	36 (0.830)	4 (0.090)	4/276	5.9	10.6%	coding (665/729 nt)	mtgA	biosynthetic peptidoglycan transglycosylase
	?	NC_000913	= 3349177	32 (0.760)					coding (631/729 nt)	mtgA	biosynthetic peptidoglycan transglycosylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCG  >  NC_000913/3349035‑3349316                                                                                                                                               |                                                                                                                                            cAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCga                                                                                                                                             <  2:608120/143‑1 (MQ=255)  aaCTTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                                                            <  2:268467/143‑1 (MQ=255)  aaCCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgag                                                                                                                                            <  2:648363/143‑1 (MQ=255)         ggagaGCAAAACGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTGGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACtt                                                                                                                                     <  1:410520/142‑1 (MQ=255)          gagaGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACttt                                                                                                                                    >  2:419634/1‑143 (MQ=255)                  aaCGCTGGCCCTCGCTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGaa                                                                                                                            <  2:418011/143‑1 (MQ=255)                  aaCGCTGGCCCTCACTTCGCGCGCAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGCCGGCTACACACGTACCGCGATGACGAGGAGACGTTCAAACGat                                                                                                                            >  1:79276/1‑142 (MQ=255)                            cTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATACACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGAGGCGACGTTGAAACGAAGAGGACTAgc                                                                                                                  >  1:75638/1‑142 (MQ=255)                                 ttCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCGACTTTGAAACGAAGTGGATTAGGTAATa                                                                                                             >  2:125792/1‑143 (MQ=255)                                            cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGACGTGGATTAGGGAATAGAGCTGCCAGt                                                                                                  >  1:426972/1‑143 (MQ=255)                                            cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGt                                                                                                  >  2:271429/1‑143 (MQ=255)                                            cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGt                                                                                                  >  2:96225/1‑143 (MQ=255)                                                gtCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATg                                                                                              <  2:723584/142‑1 (MQ=255)                                                    aGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGCTGCTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAgg                                                                                          >  2:45606/1‑142 (MQ=255)                                                                    tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTCGCCCGATGGCGACGCGACTTTGAAACGAAGTGGATTAGGTAATACATCTGCCAGTAATGCAGCTTCCGACCGGGaaaat                                                                          >  2:529917/1‑141 (MQ=255)                                                                    tAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAGTACAGCTGCCAGTAATGCAGCTTCCGCCCGGGTAAGt                                                                          >  2:600919/1‑143 (MQ=255)                                                                     aaaCGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGtt                                                                         >  2:741933/1‑143 (MQ=255)                                                                         ggatCACCGCCTAACTCATACATCTGCCGTAAATTCCCCGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCt                                                                     <  1:33767/140‑1 (MQ=255)                                                                              accgtcggaCTCATACAGCCGCCGTAAAATCCACGCCGGATGGCTAGGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTATTACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                                                                <  2:728148/135‑1 (MQ=255)                                                                              aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGCCTTTGAAACGAAGCGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCCcgcgc                                                                >  1:133756/1‑142 (MQ=255)                                                                              aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                                                                >  2:230421/1‑143 (MQ=255)                                                                              aCCGCCTAACTGATACAGCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCgg                                                                <  1:709888/143‑1 (MQ=255)                                                                                    taactgcgcccgccgCCGTGAAGTCCGGGCCTGACGGGTAGGCACGTAGCGCGAGGGCGAGGAGACTTTGAAACGAAGGGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTtgt                                                          <  1:43570/129‑1 (MQ=255)                                                                                      aCTGATACATCTGCCGTAAAATACACGCATGACGGGTACGCACGTAGCACGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCGAGTAATGCAGCTTAGGAGCGGGTAAGTTTGCTAGCGGGAtggtgg                                                        >  2:257511/1‑143 (MQ=255)                                                                                              aTCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCGGTAATGCAGCTACCGACAGGGGAAGTTAGCTCGCGGGGTTGTGGAaacaaca                                                >  1:514860/1‑142 (MQ=255)                                                                                                   ccGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTtgtg                                           <  1:52328/143‑1 (MQ=255)                                                                                                      tAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCATTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCg                                        >  1:373970/1‑143 (MQ=255)                                                                                                        aagtgcgcGCCTGAGGGCTGCGCAGGTAGCGCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATGCAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCa                                      <  1:340565/136‑1 (MQ=255)                                                                                                         aacccAGGCGTGGCGGGTACGCAGGTAGGCCGAGGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg                                     <  2:305414/140‑1 (MQ=255)                                                                                                         aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTTTGGAACTACCGTTGTGCCGCAg                                     >  1:494191/1‑143 (MQ=255)                                                                                                         aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAg                                     >  2:252977/1‑143 (MQ=255)                                                                                                                     cgggCTACGTGCGTAGCCCGATGGCGAGGAGACTTTGGACCGAAGTGGCGTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTATGTTTGCGCGCGGGATTGTGGAAATAACGTGGTGACGCAGCTTCGACGCCaa                         >  1:124080/3‑143 (MQ=255)                                                                                                                       ggCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAac                       >  1:457926/1‑143 (MQ=255)                                                                                                                                          gggCGAGGAGACCTTGAAACGAATGGGGTGAGGTAATGCGGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTGGCTCGCGGGTTGGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCgg    <  2:659699/142‑1 (MQ=255)                                                                                                                                            gCGAGGGGACTTTGGAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCg  <  1:208092/143‑1 (MQ=255)                                                                                                                                               |                                                                                                                                            CAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAATTCGGCG  >  NC_000913/3349035‑3349316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.