breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 168,628 IS2 (+) +5 bp coding (1145‑1149/2244 nt) fhuA → ferrichrome outer membrane transporter
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
RA 1,158,881 C→T S338L (TCG→TTG)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,413,461 G→A intergenic (‑329/‑9) yfbV ← / → ackA UPF0208 family inner membrane protein/acetate kinase A and propionate kinase 2
MC JC 2,722,123 Δ7 bp coding (2171‑2177/2661 nt) pka → protein lysine acetyltransferase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,771,709 IS3 (–) +3 bp coding (34‑36/363 nt) yibI ← DUF3302 family inner membrane protein
RA 3,798,659 (T)8→7 coding (654/1074 nt) waaU ← lipopolysaccharide core biosynthesis
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 123047 127528 4482 4 [3] [2] 4 [aceE]–[aceF] [aceE],[aceF]
* * ÷ NC_000913 2066274–2066464 2066577–2066466 3–304 4 [3] [2] 4 [insH1] [insH1]
* * ÷ NC_000913 3423797–3424234 3424515–3424238 5–719 4 [3] [3] 6 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =0 (0.000)20 (0.900) 20/224 0.3 97.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 1 (0.050)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078050 (0.000)17 (0.770) 17/224 0.4 97.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 1 (0.050)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1299499 =NA (NA)4 (0.160) 4/254 2.6 21.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1311375 = 15 (0.600)coding (287/720 nt) tonB membrane spanning protein in TonB‑ExbB‑ExbD transport complex
* ? NC_000913 = 1300693NA (NA)13 (0.520) 5/254 2.3 44.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1311378 16 (0.640)coding (290/720 nt) tonB membrane spanning protein in TonB‑ExbB‑ExbD transport complex
* ? NC_000913 1954549 =14 (0.550)4 (0.170) 4/240 2.4 23.4% intergenic (+136/+29) cmoB/torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
?NC_000913 1954569 = 13 (0.550)intergenic (+156/+9) cmoB/torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
* ? NC_000913 2906594 =24 (0.950)5 (0.220) 6/230 1.8 22.0% intergenic (+11/+49) ygcG/eno TPM domain protein, putative phosphatase/enolase
?NC_000913 2906624 = 14 (0.620)intergenic (+41/+19) ygcG/eno TPM domain protein, putative phosphatase/enolase