New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3618495 =48 (1.290)4 (0.120) 4/236 4.3 7.6% coding (720/807 nt) nikE nickel ABC transporter ATPase
?NC_000913 3618580 = 52 (1.510)coding (805/807 nt) nikE nickel ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGA  >  NC_000913/3618350‑3618637
                                                                                                                                                 |                                                                                                                                              
gCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAAcc                                                                                                                                             >  5:90719/1‑149 (MQ=255)
       gAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCct                                                                                                                                      >  1:150901/1‑149 (MQ=255)
        aaaaaGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCctc                                                                                                                                     >  5:143798/1‑149 (MQ=255)
               tACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCctct                                                                                                                                    >  1:87876/1‑143 (MQ=255)
               tACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCctct                                                                                                                                    <  2:87876/143‑1 (MQ=255)
                    caacaGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACg                                                                                                                         >  8:128877/1‑149 (MQ=255)
                    caacaGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACg                                                                                                                         >  2:26537/1‑149 (MQ=255)
                       caGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACgtgt                                                                                                                      <  1:26537/149‑1 (MQ=255)
                                aCCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACaaaa                                                                                                             <  7:128877/149‑1 (MQ=255)
                                   gcctgcctGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgc                                                                                                          <  6:19000/149‑1 (MQ=255)
                                           gTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCgg                                                                                                        <  6:72900/143‑1 (MQ=255)
                                           gTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCgg                                                                                                        >  5:72900/1‑143 (MQ=255)
                                           gTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTAATGGACAACGGACAACTCGTCGAACCCCAGGTGGTGGGAGAGAAAGTAACCTTTTCCTCTGACGCGGGACGTGGGCTACACACCGCGGTAttcc                                                                                                  >  8:50054/1‑147 (MQ=255)
                                                  aCCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCAtt                                                                                           >  6:75199/1‑149 (MQ=255)
                                                     cACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTccc                                                                                        >  5:52766/1‑149 (MQ=255)
                                                     cACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGAATTCCCGCATTcccc                                                                                       >  2:119730/1‑149 (MQ=255)
                                                        gACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACTCCTGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAATTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGt                                                                                     <  7:50054/149‑1 (MQ=255)
                                                                 cTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         >  4:82913/1‑120 (MQ=255)
                                                                 cTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         <  3:82913/120‑1 (MQ=255)
                                                                       gAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         >  7:119632/1‑114 (MQ=255)
                                                                       gAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         <  1:23431/114‑1 (MQ=255)
                                                                       gAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         <  8:119632/114‑1 (MQ=255)
                                                                       gAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAAcgcg                                                                                                         >  2:23431/1‑114 (MQ=255)
                                                                        aaCGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACgtg                                                                                                                       >  4:79920/1‑99 (MQ=255)
                                                                        aaCGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACgtg                                                                                                                       <  3:79920/99‑1 (MQ=255)
                                                                                tGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAgg                                                              <  6:143798/148‑1 (MQ=255)
                                                                                  ccAGAGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCAAGGTGGTGCGAGAGAAATTAACCTTTTAATCTGCCGCCGGAAGAGTGATACAAAACGCGCTATTACACGCATACCCCGTGCGCCGTCGCACCACAGAAAAGGta                                                            >  2:139098/1‑147 (MQ=255)
                                                                                     gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAAc                                                        <  5:75199/149‑1 (MQ=255)
                                                                                          tAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTCCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAAt                                                  <  1:119730/149‑1 (MQ=255)
                                                                                             tGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGGGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGc                                                <  6:90719/149‑1 (MQ=255)
                                                                                                gtATGGACAACGGACAAATCGTCGAAACCCAGGTGTTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACTTGTGCTAAAAAAAGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCaa                                              <  1:139098/147‑1 (MQ=255)
                                                                                                       cAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCAc                                      <  2:150901/149‑1 (MQ=255)
                                                                                                          cGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCAt                                   >  8:114717/1‑149 (MQ=255)
                                                                                                               aaaTCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCATCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTgc                                                                                   >  7:132445/1‑96 (MQ=255)
                                                                                                               aaaTCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCATCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTgc                                                                                   <  8:132445/96‑1 (MQ=255)
                                                                                                                  tcgtcgAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGt                                         <  2:34891/135‑1 (MQ=255)
                                                                                                                  tcgtcgAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGt                                         >  1:34891/1‑135 (MQ=255)
                                                                                                                       gAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCaa                                                    >  4:13420/1‑119 (MQ=255)
                                                                                                                       gAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCaa                                                    <  3:13420/119‑1 (MQ=255)
                                                                                                                           cccAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTc                                                                             <  5:111611/90‑1 (MQ=255)
                                                                                                                           cccAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTc                                                                             >  6:111611/1‑90 (MQ=255)
                                                                                                                            ccAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACATGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGAtt                  >  3:141973/1‑148 (MQ=255)
                                                                                                                                gtggtgGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTg             <  7:114717/149‑1 (MQ=255)
                                                                                                                                        agagaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga           <  7:51431/143‑1 (MQ=255)
                                                                                                                                        agagaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga           >  8:51431/1‑143 (MQ=255)
                                                                                                                                            aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAg                                                                    <  2:29023/82‑1 (MQ=255)
                                                                                                                                            aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAg                                                                    >  1:29023/1‑82 (MQ=255)
                                                                                                                                            aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGa  >  4:152254/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGA  >  NC_000913/3618350‑3618637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.