New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3618495 =48 (1.290)4 (0.120) 4/236 4.3 7.6% coding (720/807 nt) nikE nickel ABC transporter ATPase
?NC_000913 3618580 = 52 (1.510)coding (805/807 nt) nikE nickel ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCAC  >  NC_000913/3618435‑3618721
                                                                                                                                                 |                                                                                                                                             
gCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAAc                                                                                                                                            <  5:75199/149‑1 (MQ=255)
     tAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTCCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAAt                                                                                                                                      <  1:119730/149‑1 (MQ=255)
        tGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGGGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGc                                                                                                                                    <  6:90719/149‑1 (MQ=255)
           gtATGGACAACGGACAAATCGTCGAAACCCAGGTGTTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACTTGTGCTAAAAAAAGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCaa                                                                                                                                  <  1:139098/147‑1 (MQ=255)
                  cAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCAc                                                                                                                          <  2:150901/149‑1 (MQ=255)
                     cGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCAt                                                                                                                       >  8:114717/1‑149 (MQ=255)
                             tcgtcgAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGt                                                                                                                             >  1:34891/1‑135 (MQ=255)
                             tcgtcgAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGt                                                                                                                             <  2:34891/135‑1 (MQ=255)
                                  gAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCaa                                                                                                                                        >  4:13420/1‑119 (MQ=255)
                                  gAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCaa                                                                                                                                        <  3:13420/119‑1 (MQ=255)
                                       ccAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACATGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGAtt                                                                                                      >  3:141973/1‑148 (MQ=255)
                                           gtggtgGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTg                                                                                                 <  7:114717/149‑1 (MQ=255)
                                                   agagaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga                                                                                               <  7:51431/143‑1 (MQ=255)
                                                   agagaAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga                                                                                               >  8:51431/1‑143 (MQ=255)
                                                       aaaTTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGa                                                                                      >  4:152254/1‑148 (MQ=255)
                                                                ttttCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 <  4:55259/96‑1 (MQ=255)
                                                                ttttCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 >  3:55259/1‑96 (MQ=255)
                                                                ttttCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGc                                                                            >  8:101289/1‑149 (MQ=255)
                                                                         gACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCACCGAGTCCCCATCCCGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGcgtcgt                                                                   >  7:43131/1‑149 (MQ=255)
                                                                          aCGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGcgtcgt                                                                   <  6:52766/148‑1 (MQ=255)
                                                                          aCGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTg                                                                  <  7:101289/149‑1 (MQ=255)
                                                                          aCGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTg                                                                  >  2:185636/1‑149 (MQ=255)
                                                                                      tgCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTg                                                                  <  2:48095/137‑1 (MQ=255)
                                                                                      tgCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTg                                                                  >  1:48095/1‑137 (MQ=255)
                                                                                      tgCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATaacaac                                                       <  3:152254/148‑1 (MQ=255)
                                                                                              aaCGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACa                                                                                    <  6:66965/111‑1 (MQ=255)
                                                                                              aaCGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACa                                                                                    >  5:66965/1‑111 (MQ=255)
                                                                                              aaCGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGaa                                              <  4:141973/149‑1 (MQ=255)
                                                                                                 gcgGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGc                                                                                                                                          <  8:152821/54‑1 (MQ=255)
                                                                                                 gcgGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGc                                                                                                                                          >  7:152821/1‑54 (MQ=255)
                                                                                                     tATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 <  1:97486/59‑1 (MQ=255)
                                                                                                     tATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 >  2:97486/1‑59 (MQ=255)
                                                                                                     tATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCCTCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATcc                                       >  4:90435/1‑149 (MQ=255)
                                                                                                          cccGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 >  1:38305/1‑54 (MQ=255)
                                                                                                          cccGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAAc                                                                                                                                 <  2:38305/54‑1 (MQ=255)
                                                                                                          cccGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGa                                                                              <  7:85888/105‑1 (MQ=255)
                                                                                                          cccGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGa                                                                              >  8:85888/1‑105 (MQ=255)
                                                                                                           ccGCATTCCCCGTGCGCACTCGAAACACAGAAAAGGTATAAAGCAAATGCAACGAGTCACCATCACGCTTGATGACTATTAACTGGAGACGCTGGACAGCCTGAGACAGCTACGTGGTTATAACAACCGTTCCTAAGCTATCCGCGACa                                 >  6:137093/1‑149 (MQ=255)
                                                                                                             gCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACAtt                               >  8:111276/1‑149 (MQ=255)
                                                                                                               aTTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGGCGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATcc                                       >  7:41800/1‑139 (MQ=255)
                                                                                                               aTTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATcc                                       <  8:41800/139‑1 (MQ=255)
                                                                                                                    ccGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga                                                                                               <  6:41209/78‑1 (MQ=255)
                                                                                                                    ccGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGga                                                                                               >  5:41209/1‑78 (MQ=255)
                                                                                                                    ccGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCg                          >  8:3508/1‑147 (MQ=255)
                                                                                                                                  accacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGgcgc            >  6:143450/1‑147 (MQ=255)
                                                                                                                                  accacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCa           >  8:38063/1‑148 (MQ=255)
                                                                                                                                   ccacAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGgcgc            >  8:117138/1‑146 (MQ=255)
                                                                                                                                    cacaGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCaa          >  8:93298/1‑147 (MQ=255)
                                                                                                                                     acaGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAagag       <  1:185636/149‑1 (MQ=255)
                                                                                                                                      caGAAAAGGATTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAgg      <  5:137093/149‑1 (MQ=255)
                                                                                                                                       aGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCt                             <  4:74971/125‑1 (MQ=255)
                                                                                                                                       aGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCt                             >  3:74971/1‑125 (MQ=255)
                                                                                                                                          aaaGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCAc  <  5:143450/149‑1 (MQ=255)
                                                                                                                                                ttAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAg                                             <  2:58115/100‑1 (MQ=255)
                                                                                                                                                ttAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAg                                             >  1:58115/1‑100 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTTAACGCAAATGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCAC  >  NC_000913/3618435‑3618721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.