mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L001_R1_001.good.fq | 149,056 | 21,390,127 | 100.0% | 143.5 bases | 149 bases | 98.5% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L001_R2_001.good.fq | 149,056 | 21,310,776 | 100.0% | 143.0 bases | 149 bases | 99.2% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L002_R1_001.good.fq | 115,052 | 16,483,264 | 100.0% | 143.3 bases | 149 bases | 98.2% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L002_R2_001.good.fq | 115,052 | 16,421,055 | 100.0% | 142.7 bases | 149 bases | 98.6% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L003_R1_001.good.fq | 129,813 | 18,573,487 | 100.0% | 143.1 bases | 149 bases | 98.4% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L003_R2_001.good.fq | 129,813 | 18,504,003 | 100.0% | 142.5 bases | 149 bases | 99.1% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L004_R1_001.good.fq | 110,020 | 15,732,527 | 100.0% | 143.0 bases | 149 bases | 98.2% |
| errors | Plate-2-BOP-27-ACEF-ALE-4-flask-40_S8_L004_R2_001.good.fq | 110,020 | 15,674,593 | 100.0% | 142.5 bases | 149 bases | 98.5% |
| total | 1,007,882 | 144,089,832 | 100.0% | 143.0 bases | 149 bases | 98.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 30.2 | 3.4 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 2174 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 223 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.014 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.90591 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:41:57 25 Feb 2020 | 07:42:13 25 Feb 2020 | 16 seconds |
| Read alignment to reference genome | 07:42:14 25 Feb 2020 | 07:44:19 25 Feb 2020 | 2 minutes 5 seconds |
| Preprocessing alignments for candidate junction identification | 07:44:19 25 Feb 2020 | 07:44:34 25 Feb 2020 | 15 seconds |
| Preliminary analysis of coverage distribution | 07:44:34 25 Feb 2020 | 07:45:07 25 Feb 2020 | 33 seconds |
| Identifying junction candidates | 07:45:07 25 Feb 2020 | 07:45:08 25 Feb 2020 | 1 second |
| Re-alignment to junction candidates | 07:45:08 25 Feb 2020 | 07:45:49 25 Feb 2020 | 41 seconds |
| Resolving best read alignments | 07:45:49 25 Feb 2020 | 07:46:12 25 Feb 2020 | 23 seconds |
| Creating BAM files | 07:46:12 25 Feb 2020 | 07:46:42 25 Feb 2020 | 30 seconds |
| Tabulating error counts | 07:46:42 25 Feb 2020 | 07:46:53 25 Feb 2020 | 11 seconds |
| Re-calibrating base error rates | 07:46:53 25 Feb 2020 | 07:46:55 25 Feb 2020 | 2 seconds |
| Examining read alignment evidence | 07:46:55 25 Feb 2020 | 07:49:15 25 Feb 2020 | 2 minutes 20 seconds |
| Polymorphism statistics | 07:49:15 25 Feb 2020 | 07:49:15 25 Feb 2020 | 0 seconds |
| Output | 07:49:15 25 Feb 2020 | 07:49:25 25 Feb 2020 | 10 seconds |
| Total | 7 minutes 27 seconds | ||
