New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1604785 =	34 (1.370)	4 (0.170)	4/256	2.6	10.6%	coding (739/993 nt)	lsrD	autoinducer 2 import system permease protein
	?	NC_000913	1604815 =	35 (1.490)					coding (769/993 nt)	lsrD	autoinducer 2 import system permease protein
Rejected: Frequency below/above cutoff threshold.

CGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTC  >  NC_000913/1604671‑1604955                                                                                                                                                  |                                                                                                                                             cGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCaa                                                                                                                                          >  3:153145/1‑149 (MQ=255)         acctgatttggcactcctgccatttgcaaaccttgttgcaaatatcccactaataaaaccgcaatggcggtgccgataatggaaccggaaccaccataaatattggccccgccatcaccgccgtggtgcttggcgggGCCAATATTTAt                                                                                                                                   <  1:107479‑M2/12‑1 (MQ=255)         aTGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGTGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTAt                                                                                                                                   <  5:7161/148‑1 (MQ=255)                       ccATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttccggttc                                                                                                                     <  4:149519/148‑1 (MQ=255)                             cGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATc                                                                                                              <  1:4893/149‑1 (MQ=255)                                   tGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        >  5:134280/1‑149 (MQ=255)                                        tCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCAt                                                                                                   >  5:110753/1‑149 (MQ=255)                                          tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        <  4:158942/142‑1 (MQ=255)                                          tGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCAcc                                                                                                        >  3:158942/1‑142 (MQ=255)                                               tCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATAGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            <  6:110753/149‑1 (MQ=255)                                                           tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                                                           <  4:79047/106‑1 (MQ=255)                                                           tgctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTggttc                                                                                                                           >  3:79047/1‑106 (MQ=255)                                                             ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                                                      >  2:61781/1‑141 (MQ=255)                                                             ctgGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTttatta                                                                                      <  1:61781/141‑1 (MQ=255)                                                                     gtgttttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGcaa                                                                       >  1:5684/4‑148 (MQ=255)                                                                         tttGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAgg                                                                  >  4:60453/1‑149 (MQ=255)                                                                            ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                                                              <  1:10381/134‑1 (MQ=255)                                                                            ggTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGata                                                                              >  2:10381/1‑134 (MQ=255)                                                                             gTTCAGCACGTTCCGATCTCGGTGCGTCGATTCTGATGACCGCAAT‑CACAGCCGTGGTGCTTGGCGGGGCCAATATTAATGGAGGTTCCGCTTACATTATCGGCACCGCCATTGCGGTTGTATAAGTCGGATATTTGCAACAAGGttag                                                              >  2:123389/1‑147 (MQ=255)                                                                                  gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCa                                                                                                                   <  2:133909/91‑1 (MQ=255)                                                                                  gCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCAGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAAt                                                         <  2:5684/149‑1 (MQ=255)                                                                                   cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                                                   >  1:26150/1‑122 (MQ=255)                                                                                   cACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTg                                                                                   <  2:26150/122‑1 (MQ=255)                                                                                    aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                                                       <  3:60453/149‑1 (MQ=255)                                                                                    aCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATgg                                                       >  3:69348/1‑149 (MQ=255)                                                                                      gTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCa                                                     >  6:59202/1‑149 (MQ=255)                                                                                           gATCTCGGTGCGTCGTTTCTGATTCCCGACAA‑AACCGCCGTGGTGCTTGGCGGGGCAAATATTTATGGTGGTTCCGGTGCCATTATCGGCACCGCCATAGCGGTTTTATAAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAgg                                                >  6:106213/1‑148 (MQ=255)                                                                                             tctcGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                                                      >  3:100330/1‑93 (MQ=255)                                                                                             tctcGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGc                                                                                                      <  4:100330/93‑1 (MQ=255)                                                                                                 ggTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGc                                              <  4:153145/145‑1 (MQ=255)                                                                                                       tcgtTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg                                      <  1:5968/147‑1 (MQ=255)                                                                                                       tcgtTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAg                                      >  2:5968/1‑147 (MQ=255)                                                                                                       tcgtTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt                                    <  6:134280/149‑1 (MQ=255)                                                                                                       ataaatattggcCCCGCCAAGCACCACGGCGGTGAT‑GGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGgt                                    <  1:107479‑M1/137‑1 (MQ=255)                                                                                                                    cccgccaagcaccacggcggtgatggcgggGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCg                                                                                               <  2:3604‑M2/48‑1 (MQ=255)                                                                                                                    cccgccaagcaccacggcggtgatggcgggGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCg                                                                                               >  1:3604‑M2/31‑78 (MQ=255)                                                                                                                           agcaccacggcggtgatggcgggGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGt                 <  2:107479‑M2/126‑1 (MQ=255)                                                                                                                              ccgccgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            >  5:60266/1‑71 (MQ=255)                                                                                                                              ccgccgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGtt                                                                                            <  6:60266/71‑1 (MQ=255)                                                                                                                                          ttGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTAtcgtc  >  4:144890/1‑149 (MQ=255)                                                                                                                                           tggcgggGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGgtgcgctagatcggg    >  3:43093‑M2/8‑139 (MQ=255)                                                                                                                                           tggcgggGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTgcgc             <  4:43093‑M2/130‑1 (MQ=255)                                                                                                                                                  gCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCgg                  >  5:96753/1‑125 (MQ=255)                                                                                                                                                  gCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCgg                  <  6:96753/125‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             CGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCAT‑CACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTC  >  NC_000913/1604671‑1604955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.