Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,976,197 Δ9 bp coding (1052‑1060/1131 nt) wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3976197 3976205 9 43 [0] [0] 44 wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 39761960 (0.000)41 (1.080) 34/266 0.1 100% coding (1051/1131 nt) wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
?NC_000913 3976206 = 0 (0.000)coding (1061/1131 nt) wecE TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

CGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCG  >  NC_000913/3976057‑3976338
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cgcgTGGATTAACTTTCTGAAAGGAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCAGGGTTGCGCTGCGGGTGAACACTTGGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgc                                                                                                                                             <  2:314292‑M1/143‑4 (MQ=255)
        ttAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACAACCAAAGAGAGCGAGCGCCGGCcgcggttctac                                                                                                                                     >  1:386184‑M1/1‑132 (MQ=255)
             tttCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaaccc                                                                                                                                >  2:712423‑M1/1‑127 (MQ=255)
                         gCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgccgttctacaacctgtcgcccgtcaa                                                                                                                    >  2:273690‑M1/1‑115 (MQ=255)
                         gCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACAACCGACGCAAGTGTGCTCCTGCcgctgtcctacaacctgtcgcccgccag                                                                                                                    >  2:150837‑M1/1‑115 (MQ=255)
                           ggAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGGAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaccctgtcgcccttcactc                                                                                                                  >  1:63149‑M1/1‑113 (MQ=255)
                           ggAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCccctgttctacaacctgtcgcccgtaaata                                                                                                                  >  1:404151‑M1/1‑113 (MQ=255)
                              gatcggggctgtgtTGCAGTCTATTCCGGCGCTCGGTTGGCGTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACGCCACCAACGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagc                                                                                                               <  2:390286‑M1/137‑34 (MQ=255)
                              aaTCATGGCGGTGTTTCATTACATTCTGCTGCACGTAGTCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagc                                                                                                               <  1:691241‑M1/143‑34 (MQ=255)
                                       ggTGTTTCTTTACATTCCGCTGCACGGTTGCCCTTGGGGGGAACACTTTGGTGAGTTCCACTGTGAAGATCGCTCCACCCCCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaa                                                                                                      <  2:241490‑M1/143‑43 (MQ=255)
                                          gtTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGCGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgaaaggaaattg                                                                                                   >  1:1768‑M1/1‑98 (MQ=255)
                                                  aCATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatgagcgtacggtaattgcgactttg                                                                                           >  1:594873‑M1/1‑90 (MQ=255)
                                                   cATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGCGCGCCTGCcgctgttctacaacctgtcgcccgccaatcagcggacggtaattgcgactttgt                                                                                          >  1:368196‑M1/1‑89 (MQ=255)
                                                    actccGCTGCACGGTTGTCCCCCGGGGGAACACTTTGGTGAGTTCCACAGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  1:20882‑M1/141‑56 (MQ=255)
                                                    actccGCGGCACGGTGGCCCCGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  1:632370‑M1/141‑56 (MQ=255)
                                                    aTTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgtt                                                                                         <  2:659580‑M1/143‑56 (MQ=255)
                                                      tCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttga                                                                                       >  2:665219‑M1/1‑86 (MQ=255)
                                                          cTGCACGGTCTCCCGGCGGGGGACCACTTTGGCGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaacta                                                                                   <  1:210921‑M1/143‑62 (MQ=255)
                                                              aCGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcagtcagcggacggtcattgcgactttgttgaagtacttg                                                                               >  1:150144‑M1/1‑78 (MQ=255)
                                                                ggTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttc                                                                             <  2:605788‑M1/143‑68 (MQ=255)
                                                                     ccgtgcgGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgat                                                                        <  2:505786‑M1/140‑73 (MQ=255)
                                                                      ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtgcggtgattgtgccttcggtgacgtagtgttccggata                                                                       >  1:60694‑M1/1‑70 (MQ=255)
                                                                      ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgata                                                                       >  1:157081‑M1/1‑70 (MQ=255)
                                                                      ccTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGACCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgata                                                                       <  1:229232‑M1/143‑74 (MQ=255)
                                                                        tGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtagttgcgactgtgttgacctacttttcctgatatg                                                                     >  1:516166‑M1/1‑68 (MQ=255)
                                                                                   acTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaa                                                          <  1:457630‑M1/143‑87 (MQ=255)
                                                                                    cTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaa                                                         <  2:157081‑M1/143‑88 (MQ=255)
                                                                                    cTTTCGTGAGTTGCACGGTGAAGCTCCCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacgggaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaa                                                         <  1:296318‑M1/143‑88 (MQ=255)
                                                                                     tttGGTGGGTTCCACGGTGAAGTTCGCTACCTCACCAACGAGAGCTTCTTCCTGCcgctgttctacaacctgtcgctcgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaag                                                        <  2:632143‑M1/143‑89 (MQ=255)
                                                                                               tCCACGGGGAAGCCCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtg                                              <  2:167580‑M1/143‑99 (MQ=255)
                                                                                                   cGGTGAGGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacg                                          <  2:460970‑M1/143‑103 (MQ=255)
                                                                                                    ggTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcggccttgtggccgg                                         >  2:475010‑M1/1‑40 (MQ=255)
                                                                                                        aaGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggc                                     <  1:122716‑M1/143‑108 (MQ=255)
                                                                                                        aaGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacgggaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgcgctcgtggacggcgga                                     >  1:375363‑M1/1‑36 (MQ=255)
                                                                                                             cGCTACACCACCAAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagta                                <  1:297039‑M1/143‑113 (MQ=255)
                                                                                                                    ccaccaAAGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtcctagtggccgtcggccggtacactggt                         >  2:545528‑M1/1‑24 (MQ=255)
                                                                                                                         aaaGAGAGCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttggtgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaaga                    >  1:519730‑M1/1‑19 (MQ=255)
                                                                                                                           agagagCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagatt                  <  1:127078‑M1/143‑127 (MQ=255)
                                                                                                                             agagCGAGCGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactccttttcctgttatgtcgtttgcaaaagcgtacttgtgaaaggagaccagtacaatggtcaagattgg                >  1:5306‑M1/1‑15 (MQ=255)
                                                                                                                              gagcgagcGCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggt               <  1:280941‑M1/143‑130 (MQ=255)
                                                                                                                                  gagcgCCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccg           <  2:579808‑M1/143‑134 (MQ=255)
                                                                                                                                    gcgcCTGCcgctgttctacaacctgtcgcccgtcaatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccggg         <  1:610936‑M1/143‑136 (MQ=255)
                                                                                                                                           ccgctgttctacaacctgtcgcccgtctatcagcgtacggtaattgcgactttgttgaactacttttcctgatatgtcgttggcaaaagcgtccttgtggacggcggccagtacactggtcaagattggtgccgggttactgg  <  2:6077‑M1/143‑143 (MQ=255)
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CGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCG  >  NC_000913/3976057‑3976338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.