Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,976,197	Δ9 bp	coding (1052‑1060/1131 nt)	wecE →	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3976197	3976205	9	43 [0]	[0] 44	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3976196	0 (0.000)	41 (1.080)	34/266	0.1	100%	coding (1051/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
	?	NC_000913	3976206 =	0 (0.000)					coding (1061/1131 nt)	wecE	TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976066‑3976348                                                                                                                                             |                                                                                                                                               cgcgtggattaactttctgaaaggagcggaaatcatggcggtgtttcattacattccgctgcagggttgcgctgcgggtgaacacttgggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGc                                                                                                                                              <  2:314292‑M2/3‑1 (MQ=255)         ttaactttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccaaagagagcgagcgccggcCGCGGTTCTAc                                                                                                                                      >  1:386184‑M2/133‑143 (MQ=255)              tttctgaaagaagcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAaccc                                                                                                                                 >  2:712423‑M2/128‑142 (MQ=255)                          gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCCGTTCTACAACCTGTCGCCCGTCaa                                                                                                                     >  2:273690‑M2/116‑143 (MQ=255)                          gcggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacacaaccgacgcaagtgtgctcctgcCGCTGTCCTACAACCTGTCGCCCGCcag                                                                                                                     >  2:150837‑M2/116‑142 (MQ=255)                            ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtggagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACACCCTGTCGCCCTTCACTc                                                                                                                   >  1:63149‑M2/114‑143 (MQ=255)                            ggaaatcatggcggtgtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCCCTGTTCTACAACCTGTCGCCCGTAAata                                                                                                                   >  1:404151‑M2/114‑142 (MQ=255)                               gatcggggctgtgttgcagtctattccggcgctcggttggcgtgcgggggaacactttggtgagttccacggtgaagatcgctacgccaccaacgagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  2:390286‑M2/33‑1 (MQ=255)                               aatcatggcggtgtttcattacattctgctgcacgtagtccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGc                                                                                                                <  1:691241‑M2/33‑1 (MQ=255)                                        ggtgtttctttacattccgctgcacggttgcccttggggggaacactttggtgagttccactgtgaagatcgctccacccccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTaa                                                                                                       <  2:241490‑M2/42‑1 (MQ=255)                                           gtttcattacattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagcgagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGAAAGGAAATTg                                                                                                    >  1:1768‑M2/99‑143 (MQ=255)                                                   acattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATGAGCGTACGGTAATTGCGACTttg                                                                                            >  1:594873‑M2/91‑143 (MQ=255)                                                    cattccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgcgcgcctgcCGCTGTTCTACAACCTGTCGCCCGCCAATCAGCGGACGGTAATTGCGACTttgt                                                                                           >  1:368196‑M2/90‑143 (MQ=255)                                                     attccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  2:659580‑M2/55‑1 (MQ=255)                                                     actccgctgcacggttgtcccccgggggaacactttggtgagttccacagtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  1:20882‑M2/55‑1 (MQ=255)                                                     actccgcggcacggtggccccgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTttgtt                                                                                          <  1:632370‑M2/55‑1 (MQ=255)                                                       tccgctgcacggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGa                                                                                        >  2:665219‑M2/87‑143 (MQ=255)                                                           ctgcacggtctcccggcgggggaccactttggcgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAacta                                                                                    <  1:210921‑M2/61‑1 (MQ=255)                                                               acggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAGTCAGCGGACGGTCATTGCGACTTTGTTGAAGTActtg                                                                                >  1:150144‑M2/79‑142 (MQ=255)                                                                 ggttgccctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTc                                                                              <  2:605788‑M2/67‑1 (MQ=255)                                                                      ccgtgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGat                                                                         <  2:505786‑M2/72‑1 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTGCGGTGATTGTGCCTTCGGTGACGTAGTGTTCCGGata                                                                        >  1:60694‑M2/71‑143 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        >  1:157081‑M2/71‑143 (MQ=255)                                                                       cctgcgggggaacactttggtgagttccacggtgaagaccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGata                                                                        <  1:229232‑M2/73‑1 (MQ=255)                                                                         tgcgggggaacactttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAGTTGCGACTGTGTTGACCTACTTTTCCTGATATg                                                                      >  1:516166‑M2/69‑143 (MQ=255)                                                                                    actttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaa                                                           <  1:457630‑M2/86‑1 (MQ=255)                                                                                     ctttggtgagttccacggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  2:157081‑M2/87‑1 (MQ=255)                                                                                     ctttcgtgagttgcacggtgaagctccctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCaaaa                                                          <  1:296318‑M2/87‑1 (MQ=255)                                                                                      tttggtgggttccacggtgaagttcgctacctcaccaacgagagcttcttcctgcCGCTGTTCTACAACCTGTCGCTCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAg                                                         <  2:632143‑M2/88‑1 (MQ=255)                                                                                                tccacggggaagcccgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTtgtg                                               <  2:167580‑M2/98‑1 (MQ=255)                                                                                                    cggtgaggatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGAcg                                           <  2:460970‑M2/102‑1 (MQ=255)                                                                                                     ggtgaagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGGCCTTGTGGCcgg                                          >  2:475010‑M2/41‑143 (MQ=255)                                                                                                         aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACggcggc                                      <  1:122716‑M2/107‑1 (MQ=255)                                                                                                         aagatcgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGGAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGCGCTCGTGGacggcgga                                      >  1:375363‑M2/37‑142 (MQ=255)                                                                                                              cgctacaccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTa                                 <  1:297039‑M2/112‑1 (MQ=255)                                                                                                                     ccaccaaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTAGTGGCCGTCGGCCGGTACACTGGt                          >  2:545528‑M2/25‑143 (MQ=255)                                                                                                                          aaagagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGGTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGa                     >  1:519730‑M2/20‑143 (MQ=255)                                                                                                                            agagagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGAtt                   <  1:127078‑M2/126‑1 (MQ=255)                                                                                                                              agagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTCCTTTTCCTGTTATGTCGTTTGCAAAAGCGTACTTGTGAAAGGAGACCAGTACAATGGTCAAGATTgg                 >  1:5306‑M2/16‑143 (MQ=255)                                                                                                                               gagcgagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGt                <  1:280941‑M2/129‑1 (MQ=255)                                                                                                                                   gagcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCg            <  2:579808‑M2/133‑1 (MQ=255)                                                                                                                                     gcgcctgcCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCggg          <  1:610936‑M2/135‑1 (MQ=255)                                                                                                                                            cCGCTGTTCTACAACCTGTCGCCCGTCTATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTgg   <  2:6077‑M2/142‑1 (MQ=255)                                                                                                                                             cGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGACAAAGTGGACGGCGGCAAGTACGCGGCTCAAGATTGGTGCCGGGTTACTgca  >  2:7093/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                               TAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAGCGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACTACTTTTCCTGATATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGT  >  NC_000913/3976066‑3976348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑