New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1237780 =35 (0.870)3 (0.080) 3/274 6.0 7.8% coding (210/1299 nt) dadA D‑amino acid dehydrogenase
?NC_000913 1237791 = 37 (0.940)coding (221/1299 nt) dadA D‑amino acid dehydrogenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGA  >  NC_000913/1237653‑1237928
                                                                                                                                          |                                                                                                                                         
tCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGGGCCGCGAATGCGGGGCAAATCTCCCCCGGAAAAGCTGCACCGTGGGCGGCACCAGGCGTGCCTTTAAAAGCGATCAGATGGACGGTCCAGCGCCACGcgccgcgg                                                                                                                                       >  2:18582/1‑141 (MQ=255)
   ttGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCg                                                                                                                                    <  1:48403/143‑1 (MQ=255)
     gATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAGATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGCTTAAATGGATGTTCCAGCGCCATGCGCAGCcggcggc                                                                                                                                  >  2:734827/1‑142 (MQ=255)
      aTCGGGAGCCGGGGGCGGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCGCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGtt                                                                                                                                 <  1:554064/143‑1 (MQ=255)
       tCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCGTTTCAAAGCGATTAACTGGATGTTCCAGCGCCATGCGCCGGTGGCGGTTc                                                                                                                                >  2:497889/1‑143 (MQ=255)
         gggAGCCGGGGGCCGCCCTGGAAACCAGCGCCGCGAATGCCGGGCAAATCGCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGt                                                                                                                              <  1:142534/143‑1 (MQ=255)
           gAGCCGGGGGCCGCCCTGGAAACCAGGGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGtct                                                                                                                            <  2:283112/143‑1 (MQ=255)
               cGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGCGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGCTGTTCCAGCGCCATGCGCCGCCGGCGGTTCGTCTCGAc                                                                                                                        >  2:575502/1‑143 (MQ=255)
                        cccTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACggct                                                                                                               >  1:129269/1‑140 (MQ=255)
                        cccTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCGCGACGGGACGCAg                                                                                                               >  1:345243/1‑143 (MQ=255)
                         ccTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGt                                                                                                              <  1:633139/143‑1 (MQ=255)
                           tGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATCAAATGGAAGTTCCAGCGCAATGCGCGGCGGGCGGTTCGTCTCGACGGGACGCAGTTc                                                                                                            >  2:271632/1‑143 (MQ=255)
                            ggAGACGAGTGGGGCGAGGGCGGGGCAAAGGGCCCGCGGGTAGGCTGCACGGTGGGCGGCACCAGGGGTGCCTTTGAAAGGGATTGAGTGGATGTTGCAGCGCCGTGCGCGGCTGGCGGTTCGTCTCGACGGTACGCAGTTcc                                                                                                           <  2:375605/143‑1 (MQ=255)
                             gAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCa                                                                                                          <  1:219933/143‑1 (MQ=255)
                               aaCCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCCCCAGGTGTGCCGTTACAAGCGATTAAATGGATGTTCCAGCGCCAGGCGCCGCTGGCGGTTCGCCGCGACGGTACGCAGTTCCAGt                                                                                                        >  2:462837/1‑143 (MQ=255)
                                 ccAGTGCCGCGACTGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTg                                                                                                      <  1:117536/143‑1 (MQ=255)
                                                 cGGGCAAATCTGCCGCGGGTGTGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCaaa                                                                                      <  2:4126/143‑1 (MQ=255)
                                                 cGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTACATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGCCGGTACGCAGTTCCCGTTGAAATGGATGTGGCaaa                                                                                      >  1:513357/1‑143 (MQ=255)
                                                   ggCAAATCTCCACCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATg                                                                                    <  1:68986/143‑1 (MQ=255)
                                                       actccccccgGGTTGTGCTGCACCGTGGGCGGTGCCAGGTGTGCCTTTCGATGCGATTATGTGCGTGTTTCAGCTCCATGGGCCGCTTGCGGTTCGTCTCCACGGTACTCAGTTCCAGTTTAAATGGATGTGGCAAATGTTAc                                                                                <  2:62703/138‑1 (MQ=255)
                                                        aTCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACg                                                                               <  1:535792/143‑1 (MQ=255)
                                                           tcCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTCCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAACGGTACGCaa                                                                            >  2:233765/1‑143 (MQ=255)
                                                               ccGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGc                                                                        >  1:92038/1‑143 (MQ=255)
                                                                             ccGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGCTGTGGCAAATGTTACGTAACTGCGACACCAGCCACTa                                                          >  1:131889/1‑143 (MQ=255)
                                                                               gggggCGGCACCAGGGGTGGCTTTAAAAGCGATTAAGTGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACa                                                        <  2:181180/141‑1 (MQ=255)
                                                                                    cggcACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAACGTTACGTAACTGCGACACCAGCCACTACATGGaa                                                   <  1:28128/143‑1 (MQ=255)
                                                                                        aCCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACa                                               >  1:176716/1‑143 (MQ=255)
                                                                                                    tttAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCCGCCACTACATGGAAAACAAAGGGCGGATgg                                   >  1:346509/1‑143 (MQ=255)
                                                                                                            cGATTAAATGTATGCTCCAGCTCCATGCGCCGCTGGCTGTTCGTCTCGACGTTACGCAGTTCCAGTTGAAATGGATGTGGCTAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTg                           <  1:195269/143‑1 (MQ=255)
                                                                                                               ttAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAACGGCGGATGGTGCGTCTGGCg                        >  2:563900/1‑143 (MQ=255)
                                                                                                                 aaaTGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGa                      <  1:525742/143‑1 (MQ=255)
                                                                                                                  gacggATGCTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGaa                     <  1:678152/140‑1 (MQ=255)
                                                                                                                      gATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACa                 <  1:183741/143‑1 (MQ=255)
                                                                                                                       ctGTTCCAGCGCCACGCGCCGCTGGCGGTTGGCGTCGACGGTACGCAGTGCCGGTTGAAATGGATGTGGCAAATGGCACGTAGCTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAg                <  2:439770/142‑1 (MQ=255)
                                                                                                                             cAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACGGCCGTGa          >  1:707530/1‑143 (MQ=255)
                                                                                                                                  tcATGCGGCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAACCAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGcc     <  2:106540/142‑1 (MQ=255)
                                                                                                                                     tGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGGTGAACTGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTCGCGGAATACAGCCGTGATTGCCTGa  >  2:482164/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGA  >  NC_000913/1237653‑1237928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.