Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,646,088	Δ14 bp	coding (192‑205/306 nt)	ydfV →	Qin prophage; uncharacterized protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1646088	1646101	14	61 [0]	[0] 61	ydfV	Qin prophage; uncharacterized protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1646087	0 (0.000)	61 (1.100)	55/278	0.0	100%	coding (191/306 nt)	ydfV	Qin prophage; uncharacterized protein
	?	NC_000913	1646102 =	0 (0.000)					coding (206/306 nt)	ydfV	Qin prophage; uncharacterized protein

GAAGTCAGAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGTCGATAAAGGAACGTAACACGTTTTATGCAAC  >  NC_000913/1645946‑1646227                                                                                                                                              |                                                                                                                                             gAAGTCAGAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAg                                                                                                                                             <  1:978045‑M1/143‑2 (MQ=255)    gTCAGATAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggct                                                                                                                                          <  2:623800‑M1/143‑5 (MQ=255)    gTCAGAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCGGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggct                                                                                                                                          >  2:625358‑M1/1‑139 (MQ=255)     tcggaaaGTAGGCGTACGAACGGTGATCACTGTCCGCGTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggcta                                                                                                                                         <  2:432620‑M1/140‑6 (MQ=255)     tCAGAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggcta                                                                                                                                         >  1:443492‑M1/1‑138 (MQ=255)        gAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacat                                                                                                                                      <  1:820337‑M1/143‑9 (MQ=255)         aaaGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTACATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacata                                                                                                                                     >  1:419201‑M1/1‑134 (MQ=255)             tagtCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgca                                                                                                                                 <  1:572924‑M1/143‑14 (MQ=255)                      gAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaac                                                                                                                        <  2:443492‑M1/143‑23 (MQ=255)                           gcgacccctgtccgcTCTGTGCCAGGAGCAGCCATTGCTAAGCCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttatt                                                                                                                   <  1:1037‑M1/136‑28 (MQ=255)                           gTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttact                                                                                                                   >  2:75890‑M1/1‑116 (MQ=255)                                cACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCCTCGAggctacatatgcacaactcaacttattcatct                                                                                                              >  1:785926‑M1/1‑111 (MQ=255)                                     tCCGCTTTGTGCGAGGAGCAGCCTTTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAACAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacctatgcacaactcaacttattcctctatttt                                                                                                         <  2:820661‑M1/143‑38 (MQ=255)                                      ccGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttt                                                                                                        >  1:876874‑M1/1‑105 (MQ=255)                                      ccGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttt                                                                                                        <  1:769833‑M1/143‑39 (MQ=255)                                           ttGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctt                                                                                                   <  1:578310‑M1/143‑44 (MQ=255)                                             gtgCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCGGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgcttta                                                                                                 <  2:235029‑M1/143‑46 (MQ=255)                                             gtgCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgcttta                                                                                                 <  1:722037‑M1/143‑46 (MQ=255)                                                ccAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcaccactcaacttattcatctattttttgctttagca                                                                                              >  1:931851‑M1/1‑95 (MQ=255)                                                ccAGGAGCAGCCATTGATAAGTCCATCCTGTATTGTGGAGGTCGGCGCGTTGTTAAAGAGTGCGGCCATCGTCTTACTGGTACAGACACCATATAggctacatacgcccaactgaacttactcatctttcgtttgctttagca                                                                                              >  2:78878‑M1/1‑95 (MQ=255)                                                 cAGGAGCAGTCATTGCTATGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcat                                                                                             <  2:609617‑M1/143‑50 (MQ=255)                                                 cAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcat                                                                                             <  1:753818‑M1/143‑50 (MQ=255)                                                   ggAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgt                                                                                           >  1:44590‑M1/1‑92 (MQ=255)                                                         gcCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGGTTTTAAAGCGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacgtaatcatctattattttctttaacatgtcagtga                                                                                     >  2:16967‑M1/1‑86 (MQ=255)                                                          ccATTGCTAAGTCCATCCTGTATGGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgtt                                                                                    <  2:614687‑M1/143‑59 (MQ=255)                                                           cATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATTggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttg                                                                                   <  2:936020‑M1/143‑60 (MQ=255)                                                            aTTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGCTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgc                                                                                  >  2:74645‑M1/1‑83 (MQ=255)                                                             ttGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgct                                                                                 >  1:157257‑M1/1‑82 (MQ=255)                                                              tGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctt                                                                                <  2:44590‑M1/143‑63 (MQ=255)                                                               gCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgcttt                                                                               <  1:621868‑M1/143‑64 (MQ=255)                                                                cTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttc                                                                              >  1:653284‑M1/1‑79 (MQ=255)                                                                         tCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgtttctttctcgtcgcag                                                                     >  2:216275‑M1/1‑70 (MQ=255)                                                                         tCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGACCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttcgcatgtcagtgttgctttctcgttggct                                                                     >  2:302714‑M1/1‑70 (MQ=255)                                                                          ccTGTATTGTGCAGGTCGGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgg                                                                    <  1:625358‑M1/143‑75 (MQ=255)                                                                            tGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggt                                                                  >  2:134742‑M1/1‑67 (MQ=255)                                                                              tATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcctctatttcttgctttagcatgtcactgctgcttcctcgcctgcgggtgc                                                                >  1:287074‑M1/1‑65 (MQ=255)                                                                                ttGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagc                                                              <  2:745878‑M1/143‑81 (MQ=255)                                                                                   tgCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggt                                                           >  1:142146‑M1/1‑60 (MQ=255)                                                                                    gCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAtgctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttttcgtcggcgggtgagcggtg                                                          <  2:174112‑M1/143‑85 (MQ=255)                                                                                    gCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttcctttctcgtcggcgggtgagcgctg                                                          >  2:864298‑M1/1‑59 (MQ=255)                                                                                      cggtAAGATCCTTTTTAATGACTTCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgac                                                        <  2:3367‑M1/142‑87 (MQ=255)                                                                                      aGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgac                                                        <  2:876874‑M1/143‑87 (MQ=255)                                                                                                gTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctggcctgcc                                              >  1:916965‑M1/1‑47 (MQ=255)                                                                                                gTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggggacctgacctgtc                                              >  1:228598‑M1/1‑47 (MQ=255)                                                                                                  ttttAAAGAGGCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcga                                            <  1:220519‑M1/143‑99 (MQ=255)                                                                                                   tttAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgat                                           >  2:170692‑M1/1‑44 (MQ=255)                                                                                                        agagTCCGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaagg                                      >  1:799553‑M1/1‑39 (MQ=255)                                                                                                          agTGCGTCCACCAGCTTACTGGTACGTACCCCATATAggctacatatgcacacctcaccttatgcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaa                                    <  1:741622‑M1/143‑107 (MQ=255)                                                                                                           gTCCGGCCATCATCTGATTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattgtctgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaac                                   <  2:881344‑M1/143‑108 (MQ=255)                                                                                                             ccGGCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgt                                 <  1:21074‑M1/143‑110 (MQ=255)                                                                                                               ggCCATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaa                               <  1:799351‑M1/143‑112 (MQ=255)                                                                                                               ggCCATCATCTTACTGGTACAGACACCATATAggctacatatgcaaaactcaacttattcatctattttttacttaagcatgtcagtgttgctttctattcagcgggtgagcggtgacctgacctttctatataggatcgtaa                               >  2:121630‑M1/1‑32 (MQ=255)                                                                                                                 ccATCATCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaaca                             <  2:142146‑M1/143‑114 (MQ=255)                                                                                                                  catcatCTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgcgtgagcggtgacatgagctcgtgagcaaggaccgtaacac                            >  2:395947‑M1/1‑29 (MQ=255)                                                                                                                      atcTTACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaacacgttt                        <  1:310009‑M1/143‑119 (MQ=255)                                                                                                                         ttACTGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaacacgttttat                     <  2:157257‑M1/143‑122 (MQ=255)                                                                                                                             tGGTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgatcaaggaacgtaacacgttttatgcaa                 >  2:40741‑M1/1‑18 (MQ=255)                                                                                                                               gTACAGCCACCGTATAggctacatatgcacaactccacttgttcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgccctgtcgataaaggaacgtaacacgttttatgcaaca               <  2:277501‑M1/143‑128 (MQ=255)                                                                                                                               gTACAGACACCATATAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaacacgttttatgcaacc               >  2:789492‑M1/1‑16 (MQ=255)                                                                                                                                          atatAggctacatatgcacaactcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaacacgttttatgcaacacccgcatgcgg    >  1:452064‑M1/1‑5 (MQ=255)                                                                                                                                            ataggctacatatgcacacctcaacttattcatctattttttgctttagcatgtcagtgttgctttctcgtcggcgggtgagcggtgacctgacctgtcgataaaggaacgtaacacgttttatgcaacacccgcatgcggca  <  1:880147‑M1/143‑141 (MQ=255)                                                                                                                                              |                                                                                                                                             GAAGTCAGAAAGTAGTCGTAAGAACGGTGATCACTGTCCGCTTTGTGCCAGGAGCAGCCATTGCTAAGTCCATCCTGTATTGTGCAGGTCAGCTCGTTTTTAAAGAGTCCGGCCATCATCTTACTGGTACAGACACCATATACTTTGTGACGGTCAGGCTACATATGCACAACTCAACTTATTCATCTATTTTTTGCTTTAGCATGTCAGTGTTGCTTTCTCGTCGGCGGGTGAGCGGTGACCTGACCTGTCGATAAAGGAACGTAACACGTTTTATGCAAC  >  NC_000913/1645946‑1646227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑