Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	46 (1.150)	39/284	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	47 (1.170)	36/284	0.1	100%	intergenic (‑110/‑495)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293171‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGC  <  NC_000913/1979270‑1979131                                                                                                                                                                                                                                                                                          CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGT                                                                                                                                           <  1:494426/143‑1     CGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATG                                                                                                                                       <  1:343901/143‑1      GATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAGTTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGC                                                                                                                                      >  2:174356/1‑143      GATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAACGCTATTTCTTGGCGGCACACGGTGATtc                                                                                                                                      >  1:94979/1‑141           cCTCCCCAGAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCA                                                                                                                                 <  1:437221/142‑1             TCCCCATAATATGTGAAATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAAC                                                                                                                               <  2:569599/143‑1             TCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACATCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCCCAAGGAGATGCTGCAAAC                                                                                                                               >  2:221466/1‑143                CCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTA                                                                                                                            >  1:725020/1‑143                    AAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGA                                                                                                                        >  2:157095/1‑143                           GACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTG                                                                                                                 <  1:328005/143‑1                              ATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGTGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTAT                                                                                                              <  2:452846/143‑1                                          gtgTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAATATATTTATTGGCGGCACAAGGTGATGCTGCCATCTTACTGATTTAGTGTATGATGGTGTTTTT                                                                                                  <  1:8691/140‑1                                          GTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTT                                                                                                  <  1:174356/143‑1                                            gTTATCCTCACGTGCGGCGAAATCATCGTTGTAAATAGGGCTATATGCCGGGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGA                                                                                                <  2:278789/142‑1                                            TTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGA                                                                                                <  1:543160/143‑1                                            TTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGA                                                                                                <  1:370118/143‑1                                             TTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAG                                                                                               <  1:319931/143‑1                                             TTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAG                                                                                               <  1:112871/143‑1                                               AACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGT                                                                                             <  1:354316/143‑1                                                gcgcCACGTGCTGCGAAAGCATCGGTGTAAATAGGGCTATATGCCGCGTGTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGAGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTG                                                                                            <  1:662421/139‑1                                                    CACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCC                                                                                        >  1:327806/1‑143                                                          CTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGC                                                                                  <  2:484301/143‑1                                                             CGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCCACTTACGGATTTAGTGTATGATGGTGTTTTTGAGGTACTCCAGTGGCTTC                                                                               >  2:474107/1‑143                                                             CGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAAGTGATGCTGCCAACTTACTGATTTAGGGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTC                                                                               >  1:367896/1‑143                                                               AAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTG                                                                             <  2:348034/143‑1                                                                 ATCAGCGGTGTAAATAGGGCTAGATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTCATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTT                                                                           <  1:573320/143‑1                                                                           TAAATACGGCTATATGCCGCGTCTTTGCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAGCTTACTGATCTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCT                                                                 <  2:89238/143‑1                                                                               TAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCC                                                             <  1:227336/143‑1                                                                                     TATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTACTG                                                       >  1:552106/1‑143                                                                                        ATGCCGCGTCTTTTCTGGCTAATTGTATGAAAAGGTATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTC                                                    <  2:297709/143‑1                                                                                         TGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCA                                                   <  1:278245/143‑1                                                                                         TGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTCTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCA                                                   <  2:417273/143‑1                                                                                                   tttcgtGCTAATTTTATGAAAAGTTATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATTATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTTCCTCCTGTTCAGCTACTGACG                                         <  2:200224/137‑1                                                                                                                TTATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAA                            <  1:338521/143‑1                                                                                                                 TATGAAAAGATATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAAC                           <  1:157095/143‑1                                                                                                                      AAAGATAGTGATTGGCGGCACAAGGTGATGCTGCCGACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAA                      <  2:492324/143‑1                                                                                                                        AGATATTTATTGGCCGCACATGGTGATGCTGGCACCTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCATTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAA                    <  2:695509/143‑1                                                                                                                            ATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTCCTGACGGGGTGGTGCGTAACGGCAAAAGCAC                >  1:27204/1‑143                                                                                                                            ATTTATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGGGTAACGGCAAAAGCAC                >  2:410722/1‑143                                                                                                                                ATTGGCGGCACAAGGTGCTGCTGCCAACTTACTGATTTAGTGTATGAGGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCC            <  1:248271/143‑1                                                                                                                                ATTGGCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGGGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCC            <  1:253402/143‑1                                                                                                                                    tCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGAC        <  1:320001/142‑1                                                                                                                                    GCGGCACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTTTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGAC        <  2:564649/143‑1                                                                                                                                         ACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGCCGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGCCATCAG   >  2:564973/1‑143                                                                                                                                         ACAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAG   <  1:498100/143‑1                                                                                                                                         ACAAGGTGATGCTGCCAACTTACTGAGTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTGCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAACAGCACCGCCGGACAGCAG   >  2:15280/1‑143                                                                                                                                          CAAGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGC  <  2:327806/143‑1                                                                                                                                                                                                                                                                                          CCTACGATAATCTCCCCATAAAATGTGACATGAATCAGGAAGTTTTAACCTCACGTGCTGCGAAATCATCGGTGTAAATAGGGCTATATGCCGCGTCTTTTCTGGCTAATTTTATGAAAAGATATTTATTGGCGGCACAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293171‑1293032 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAGC  <  NC_000913/1979270‑1979131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.