Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
JC JC | NC_000913 | 4,049,922 | IS5 (–) +4 bp | noncoding (24‑27/109 nt) | spf → | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 3652036 = | NA (NA) | 66 (1.290) | 53/284 | 0.0 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 4049925 | 0 (0.000) | noncoding (27/109 nt) | spf | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 59 (1.160) | 50/284 | 0.1 | 100% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | 4049922 = | 0 (0.000) | noncoding (24/109 nt) | spf | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
AATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/3653094‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAG > NC_000913/4049922‑4050055 AATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCT > 1:170532/1‑143 tgtcgGGTCTGAGGGCGCTTACGTCTGAATTCGCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGAC < 2:855040/138‑1 TGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTGATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTT > 1:419107/1‑143 TGAGGGCGCGTACTGCTGAGTTCACTGTCGGCGAAGGTAAGTTGATGACTCCTGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTT < 1:329332/143‑1 TGACGTCGCTTACTGCTGAATTCACTGTCGGCGCAGGTAAGTTGATGACTCATGATGAACCGTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTT < 2:31181/143‑1 CGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCAC > 1:21161/1‑143 CGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCAC < 1:359130/143‑1 TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATC > 2:494406/1‑143 TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACGCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCAGGTAATCGGAT > 1:473464/1‑143 CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTT > 1:624137/1‑143 CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTT > 1:697172/1‑143 TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTACTCGAATTTG > 2:865221/1‑143 TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTG < 2:210548/143‑1 GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCGATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGG < 2:84456/143‑1 GAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCGGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTGATACTTCACGTAATCGCATTAGG > 2:358861/1‑143 ATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCT < 1:685912/143‑1 ATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCT > 1:886294/1‑143 GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTA < 2:746960/143‑1 GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTA < 2:529732/143‑1 GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTGTACTTAACGGAATCTCATTTGGCTGCCTATGTTA > 2:287280/1‑143 GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAG < 2:817113/143‑1 GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCACTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAG > 2:516844/1‑143 GAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTATCC > 2:426575/1‑143 AAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGACTATTTTAGCCGCCCCA > 2:344148/1‑143 GATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGACTGAATATGTTAGACGCCCCAGTGAG > 2:297785/1‑143 GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAA > 1:884972/1‑143 GACTCATGATGAACCCTGGTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAA < 2:466936/143‑1 ACTCATGATGAACCCTGTTCTATGGCTCCACGTAACAATTATTATCTCATATCAGTGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAT < 2:883864/143‑1 CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATCTTAGCCGCCCCCGTCAGTGATG > 2:527823/1‑143 CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTAGCTGGATGTTTTAGCCGCCCCAGTCAGTAATG > 1:706446/1‑143 TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCTGGGACTTATTCGCACCTTCCCTATCTTTCAGACCTCTTACTTCACGTAATCGGTTTTGGCTGATTATTTTCGCCGCCCCAGCCAGTCATta > 1:813803/1‑141 TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGA > 2:451666/1‑143 TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGA > 1:385768/1‑143 ATGAACCTTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGG < 2:469391/143‑1 ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCCGTCAGTAATGACGGGG > 2:910219/1‑143 ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGGTGAATATTTTGGCCGCCCCAGTCAGTAAGGACTGGG > 1:854243/1‑143 TGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGG < 1:365455/143‑1 TGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGG > 2:494418/1‑143 GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAGTGGGGC > 2:540827/1‑143 AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCG < 2:283634/143‑1 AACCCTGTGCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCG < 2:760713/143‑1 TGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTACTGGGGCGTTTTT > 1:631138/1‑143 GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGAGTTTTTT > 1:464902/1‑143 TCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAT < 1:265627/143‑1 CTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATT > 1:717318/1‑143 GATGACAAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAA < 1:368312/143‑1 TGACAAACATGTGCTCATACGAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAA < 2:699172/143‑1 ACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGACAAGA > 1:366157/1‑143 CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACATCCAGACCAAAAAAATAACGAGATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAT < 1:569429/143‑1 AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGCTTTGGCTGCATATTTTAGACGCCCAAGACAGTGATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATC > 2:670818/1‑143 CTCATATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCC < 2:385768/143‑1 ATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGCATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATG > 1:736057/1‑143 ATCAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATG < 1:539027/143‑1 CAGGGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCG > 2:863572/1‑143 GGACTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCca > 1:432029/1‑141 CTTGTTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAGAGAATAGATGCGTAATGCCTGATGCGATTTGT > 2:290611/1‑143 TTCGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTGTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTAT > 1:904179/1‑143 CGCACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGACTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATACCTGGGGCGTTTTTTATTGAGCGCAAGAAAAGATCCGTAATGCATCATGCGCTATGTTTATCA > 2:600295/1‑143 gaACCTTCCCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCTCTCTAGACAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGACTGATGCGCTATGTGTATCAG > 1:35487/3‑143 AATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/3653094‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAG > NC_000913/4049922‑4050055 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |