Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,922 IS5 (–) +4 bp noncoding (24‑27/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3652036 =NA (NA)66 (1.290) 53/284 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4049925 0 (0.000)noncoding (27/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 3653230NA (NA)59 (1.160) 50/284 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4049922 = 0 (0.000)noncoding (24/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGG  >  NC_000913/3653096‑3653369
                                                                                                                                      |                                                                                                                                            
tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGa                                                                                                                                      >  2:667782/1‑143 (MQ=31)
  tctTTTCGGGTCTCACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGCTGAACCCTGTTCTATGGCTCCAGATCACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGaca                                                                                                                                    <  2:50037/143‑1 (MQ=255)
     tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTa                                                                                                                                 >  1:399203/1‑143 (MQ=34)
     tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCTTAGGTGACTCTa                                                                                                                                 >  1:606816/1‑143 (MQ=17)
          ggTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTAttttt                                                                                                                           <  2:701291/143‑1 (MQ=39)
            tCTGTCGTCGCTTTCTGCTGAATTCTCTGTCGGCGACCGTGGGTTGGTGACTCATGATGAGCCCTGTTCCATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTAttttttt                                                                                                                         <  1:77299/143‑1 (MQ=14)
              tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGACACTATTTTTTTAc                                                                                                                       >  2:891787/1‑143 (MQ=255)
              tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTCGGTGACACTATTTTTTTAc                                                                                                                       >  1:234712/1‑143 (MQ=255)
                aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACAt                                                                                                                     >  2:74922/1‑143 (MQ=255)
                   gcctttACGGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGTCTCTTGATGAACCCTGTTCTATGGCTCCAGATGACTTACTTTATCTCATATCAGGGACTTGTTGGTATCTTCTTTAGGTGACACTATTTTTTTACATaaa                                                                                                                  <  2:73850/139‑1 (MQ=14)
                    cgcTTACTGCTGAAGTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAg                                                                                                                 <  2:150638/143‑1 (MQ=255)
                          ctgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACAcc                                                                                                           <  1:243164/143‑1 (MQ=255)
                           tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACAccc                                                                                                          >  2:18827/1‑143 (MQ=255)
                           tgctgAATTCACTGTCGGAGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAACTTCCTTAGGTGACACTATTTTTTTGCATAAAGAACAgcc                                                                                                          >  1:189047/1‑143 (MQ=255)
                              tgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTAc                                                                                                       <  1:74922/143‑1 (MQ=255)
                              tgAATTCACTGTCGGCGAAGGGAAGTTAATAACTCATGATGTACCCTGTTCTATGGCTCCAGATGACCAACATGATCTCATATCAGGGGCTTGTCCGCCCCTTCCTTAGGTGACACTAGCTGTTTACACAAAGAACACCCTag                                                                                                       >  2:652894/1‑142 (MQ=14)
                                 aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTAcccc                                                                                                    >  1:230916/1‑143 (MQ=255)
                                     aCTGTCGGCGAAGGTACGTTCATGACTCATGATGAACCCTGTTCTACGGCTCCAGATGACAAACATGATCTCATATCAGGCACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCaaa                                                                                                <  1:682216/143‑1 (MQ=255)
                                       tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAAtt                                                                                              >  1:602898/1‑143 (MQ=255)
                                         tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCCAAttgt                                                                                            >  1:27469/1‑141 (MQ=255)
                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGAGACTATCTGTTGACAGAAAGAACACCCTACCCCCAAATtatata                                                                                         >  1:618672/1‑143 (MQ=17)
                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAACGGACCCCCTTCCCCCACATtttata                                                                                         >  2:671434/1‑143 (MQ=17)
                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATtatata                                                                                         >  1:579458/1‑143 (MQ=255)
                                            gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTATTACATAAAGAACCCCCTACCCCCAACTtatata                                                                                         >  2:859069/1‑143 (MQ=255)
                                                  gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATg                                                                                   <  2:350430/143‑1 (MQ=255)
                                                   tAAGTTGATGACTCATGATGAGCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATgg                                                                                  >  1:28994/1‑143 (MQ=255)
                                                        tgatgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAat                                                                             <  1:683273/143‑1 (MQ=255)
                                                             aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCtt                                                                        <  2:386957/143‑1 (MQ=255)
                                                               tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGa                                                                      >  1:256948/1‑143 (MQ=255)
                                                                 atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGaca                                                                    >  1:321199/1‑143 (MQ=255)
                                                                   gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGacaaa                                                                  <  2:92403/143‑1 (MQ=255)
                                                                     tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATCAAGATCGACAACATCCTGacaaaca                                                                >  2:57290/1‑143 (MQ=255)
                                                                         cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATCCAGATGGACAATATCTTGACAAACAAATc                                                            >  1:455412/1‑143 (MQ=255)
                                                                          ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTTACATAAAGAACACCCTACCCCTAAATTATATAAAGATGGACAATATCTTAAtaaacaaaac                                                            >  2:67968/1‑141 (MQ=25)
                                                                                 tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGttt                                                    <  2:798378/143‑1 (MQ=255)
                                                                                  aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTa                                                   >  1:215543/1‑143 (MQ=255)
                                                                                      cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACTCCCAAATTATATAAAGATGTACAATATCTTGACAAACAAATCGAAGGTTTAtttt                                               >  2:664418/1‑143 (MQ=255)
                                                                                      cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCACATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTAtttt                                               <  2:258276/143‑1 (MQ=255)
                                                                                         cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCaa                                            >  2:782771/1‑143 (MQ=255)
                                                                                             tGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGAAGCACAAAATCTTGTCAGACAAATCGAAGGTTTATTTTCAACatt                                        >  1:618360/1‑143 (MQ=38)
                                                                                                 aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACCttatta                                    >  1:736299/1‑143 (MQ=255)
                                                                                                     aTGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCa                                >  2:852638/1‑143 (MQ=255)
                                                                                                        aTCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTa                             <  2:28994/143‑1 (MQ=255)
                                                                                                           tcATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAACTTATATAAAGATGGACAATATCTTGACACACAAATCGAAGGTTTATTTTCAACATTATTAACCACTAATg                          >  2:634153/1‑143 (MQ=255)
                                                                                                           tcATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATg                          >  1:762478/1‑143 (MQ=255)
                                                                                                             atatCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCAAAAATTATAAAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGAc                        >  1:448099/1‑143 (MQ=255)
                                                                                                             aaacgaGGGACTTGTTCGCACCTTCCTTAGGTGACACGATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGAc                        <  1:560588/138‑1 (MQ=255)
                                                                                                                tCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAAc                     >  2:134866/1‑143 (MQ=255)
                                                                                                                    ggACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTTTTTTTTTTTCACCCTACACCCAACTTATATAAAGCTGGACAATATCTTGACAACCAAATCGAAGGTTTCTTTTCAACATTATTCTCCATCCATCACAACCttc                 >  2:802858/1‑142 (MQ=255)
                                                                                                                       cTTGTTCGCAGCTTCCTGAGGTGTCACTATTTGTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGAGGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTAc              <  2:53321/143‑1 (MQ=255)
                                                                                                                        ttGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACg             >  1:829206/1‑143 (MQ=255)
                                                                                                                           ttCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTGCGACAGTATTAACCATTAATGACAACCTTTTACGAGc          >  1:618378/1‑143 (MQ=255)
                                                                                                                           ttCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAATTTTATTTTCAACATTCTTAACCATTAGTGACAACCTTTTTCGAGc          >  2:191191/1‑143 (MQ=255)
                                                                                                                              gcagcgtccttAGGTGAGACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCaaa       <  1:190462/137‑1 (MQ=255)
                                                                                                                              gCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGCGCaag       >  1:54042/1‑142 (MQ=255)
                                                                                                                              gCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCaaa       >  1:104721/1‑143 (MQ=255)
                                                                                                                                accgcccttAGGTGATACTCTTTTCTTACCGAAAGAACACCCTACCCCCAAATTATATCAAGATGGACAATATCTTGACACACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAg     <  2:827140/138‑1 (MQ=255)
                                                                                                                                   ttccttAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCgg  >  1:679669/1‑143 (MQ=255)
                                                                                                                                      |                                                                                                                                            
TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTGACACTATTTTTTTACATAAAGAACACCCTACCCCCAAATTATATAAAGATGGACAATATCTTGACAAACAAATCGAAGGTTTATTTTCAACATTATTAACCATTAATGACAACCTTTTACGAGCAAAAGCGG  >  NC_000913/3653096‑3653369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.