Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	458,790	IS186 (–) +6 bp :: Δ1 bp	intergenic (+90/‑93)	clpX → / → lon	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	458790 =	1 (0.020)	54 (1.080)	46/278	0.1	98.1%	intergenic (+90/‑98)	clpX/lon	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
	?	NC_000913	608008 =	NA (NA)					noncoding (2/1345 nt)	IS186	repeat region
*	?	NC_000913	= 458795	0 (0.000)	35 (0.710)	32/276	0.6	100%	intergenic (+95/‑93)	clpX/lon	ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
	?	NC_000913	= 609351	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region

TAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGAT  >  NC_000913/607867‑608130                                                                                                                                              |                                                                                                                           tAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACATAATATTGAGTTTAAGGATGTTCTCGCTTACGGACCGAGGAAGTAGCCGTTGGGCGGGGGGTAATcc                                                                                                                           >  2:745162/1‑143 (MQ=255)   gTGCTGTGTTGAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGCAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCTCa                                                                                                                         <  2:873753/143‑1 (MQ=255)    tGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCAt                                                                                                                        >  1:7727/1‑143 (MQ=255)         tgtTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTCATCCCCTAAgag                                                                                                                   >  2:522019/1‑141 (MQ=255)           tttAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATACGACAAGCACt                                                                                                                 >  2:382425/1‑143 (MQ=255)             tacctgTGCTGGGATCTACGCTTCTGGTCGTAGACTCGCTGTGTGAGTTTGCGGTGAAGGAACGTAATATTGAGTGTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTaa                                                                                                               <  2:829519/138‑1 (MQ=255)             taccGGTTCTGGGATTTACGCTTCTGGTCGGAGCCTCGCTGTGTGCGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTaa                                                                                                               <  1:612848/140‑1 (MQ=255)               gtggTGCTGGGGTTTTCGCTTCTGGTCGGAGGCTCGCGTTGTGAGTTGACTGTGAAGTGACGTAATATTGAATTTAAGGCTGTGCTCGCTTACGAACCGAAGAAGTAGCTGTTGTGCGGGGAGTAATCCCATAAGCGCTAACt                                                                                                             <  2:890503/141‑1 (MQ=255)               aCGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACt                                                                                                             >  1:491210/1‑143 (MQ=255)                  gTGCTGGGATTTACGCCTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                                          <  1:137337/143‑1 (MQ=255)                  gTGCCGGGCTTTCCCCTCCTGCTCGGAGACTCGCTGTGCGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTaa                                                                                                          <  1:791579/143‑1 (MQ=255)                    gCTGGGATTTACGCTGCTGGTCGGAGACTCGCTGTGTGAGTGTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAgg                                                                                                        <  1:602332/143‑1 (MQ=255)                      tGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGt                                                                                                      >  2:862136/1‑143 (MQ=255)                      tGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGGTGTGCGGGGAGCAATCCCATAAGCGCTAACTTCAGGGt                                                                                                      >  2:727549/1‑143 (MQ=255)                          aTTTACGCGTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                                  <  1:728974/143‑1 (MQ=255)                           tttACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTgg                                                                                                 >  2:652027/1‑143 (MQ=255)                                       gTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                                     <  1:33459/143‑1 (MQ=255)                                        tctgacaCTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAATGCTGTCCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTTat                                                                                    <  1:792758/137‑1 (MQ=39)                                          ggAGAATCGCGGTGTGAGTTTACGGTGAGGGAACGTAATAGTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGatat                                                                                  <  2:425251/143‑1 (MQ=255)                                             gagtCGCTGTGTGGGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGGTGTGCGCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAt                                                                               <  2:94785/140‑1 (MQ=38)                                               cTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGACAGATATGAgtt                                                                             >  1:798746/1‑143 (MQ=255)                                                  gCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                                                                          >  1:344928/1‑143 (MQ=255)                                                  gCTGTGTGAGTTTACGGGGAAGGAACGTAATCTTGAGTTTAAGGCTGTTCTCGCTTACGTACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAc                                                                          <  2:320887/143‑1 (MQ=255)                                                         gggTTTACGGTGAAGGAACGTAATATTGAGTGTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGa                                                                   <  2:857789/141‑1 (MQ=255)                                                         gAGTTTACGGGGGAGGCGCGTAATGTTGAGTTTAGGGCTGTTCTCGCTTACGACCCGAAGGAGTAGCCGTTGGGCGGGGAGTAGTCCGATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGa                                                                   <  2:858071/143‑1 (MQ=21)                                                           gTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATg                                                                 >  1:474327/1‑143 (MQ=255)                                                            tttACGGTGAATGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                                                <  2:208046/143‑1 (MQ=255)                                                              tACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                                              <  1:753994/143‑1 (MQ=255)                                                              gaCGGTGAAGGAACGTAATATTGAGTTTTAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAt                                                              <  2:865779/142‑1 (MQ=255)                                                               acgggtaaGGAACTTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAtt                                                             <  2:226406/137‑1 (MQ=255)                                                                  gTGGAGGAACGTAATATTGAGTTTAAGGCTGTCCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                          <  1:219806/143‑1 (MQ=255)                                                                  gTGAAGGACCGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGACCCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                          <  1:126346/143‑1 (MQ=255)                                                                      aGGAACGTAAGATTGAGTTGAGGGCTGTGCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCa                                                      <  2:200498/143‑1 (MQ=255)                                                                                 atTGAGTTTAAGGGTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGt                                           <  2:281891/143‑1 (MQ=255)                                                                                 atTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGt                                           <  1:553663/143‑1 (MQ=255)                                                                                  ttGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCATGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGta                                          >  2:28540/1‑142 (MQ=255)                                                                                    gAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGGTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTGAg                                        >  2:571336/1‑143 (MQ=255)                                                                                          aaGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATCAGCGCTCACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGCTGAATTACTCTCACGATAACTGGCCAGCAATTc                                  >  1:837829/1‑143 (MQ=255)                                                                                             gCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTgg                               <  1:652027/143‑1 (MQ=255)                                                                                              cggtgctcGCTTACGAACCGAAGAAGTAGCCGGTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGTTTGGTGTAGTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                              <  1:128343/138‑1 (MQ=18)                                                                                              cTGGTCCCGCTTACGAACCGAAGAAGTAGCCGTGGGGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                              <  1:469669/143‑1 (MQ=255)                                                                                                 ttCGCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCa                           <  2:271682/143‑1 (MQ=255)                                                                                                     cccTTACGAACCGAAGAAGCCGCTGTTGTGCGGGGAGTCCTCTCCTAAGCTCTCACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGACTTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATAtt                       <  1:205809/141‑1 (MQ=14)                                                                                                     cGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACCCCCACGATAACTGGTCAGCAATTCTGGCCCATCtt                       >  2:209314/1‑143 (MQ=255)                                                                                                     cGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGACTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGCTTTAACGTGCCGATGAGTTACTCTCACGATAACTAGTGATCAAGTCTGGCCAATCtt                       >  2:349426/1‑143 (MQ=18)                                                                                                      gCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCGATAGTg                      >  1:7520/1‑143 (MQ=255)                                                                                                      gCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATGTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTg                      <  1:123469/143‑1 (MQ=255)                                                                                                            gAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGGATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCCATTCTGGCCCATATGGGTAAGc                >  1:787698/1‑143 (MQ=255)                                                                                                              aCCGAAGAAGTAGCCGTTGTGCGGGGGGTAATCCCATAAGCACTAACTGAAGGGTTGTGGTATTACGCCTGAGATGATTTAATGTGCCGATGAATTACTCTCCCCATAACTCGTCCGAAATTCTGGCAGATACTAGTAAGccc              >  2:10344/1‑143 (MQ=17)                                                                                                                         aGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGa   <  1:831440/143‑1 (MQ=255)                                                                                                                         aGCCGTCGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGa   <  1:86879/143‑1 (MQ=255)                                                                                                                          gCCGTTGTGCGGGGAGTAATCGCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCGGATATGATTTGACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCACCTTGGTCCGCCCGCAGCACTggct  >  1:447461/1‑141 (MQ=14)                                                                                                                                              |                                                                                                                           TAGTGCTGTGTTTAACGGTGCTGGGATTTACGCTTCTGGTCGGAGACTCGCTGTGTGAGTTTACGGTGAAGGAACGTAATATTGAGTTTAAGGCTGTTCTCGCTTACGAACCGAAGAAGTAGCCGTTGTGCGGGGAGTAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGAT  >  NC_000913/607867‑608130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.