Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	32 [0]	[0] 32	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	32 (0.790)	27/284	0.5	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001504‑4001781                                                                                                                                             |                                                                                                                                          gTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTACTTTGAAGACGACGACGGCCGGCATct                                                                                                                                         >  1:648021‑M1/1‑141 (MQ=255)           aTCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttctt                                                                                                                               <  1:465128‑M1/143‑13 (MQ=255)            tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAGCTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttc                                                                                                                              >  1:678700‑M1/1‑130 (MQ=255)            tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTTGCAACCCGCGCGGAACTGGAAGACATCGAAGCATGGGCACGGTTATTTCAAGACGACGACGGCCTGCATctcctgattcttc                                                                                                                              >  2:283421‑M1/1‑130 (MQ=255)            tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttc                                                                                                                              >  1:675534‑M1/1‑130 (MQ=255)              ttGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCGACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctt                                                                                                                            >  1:315507‑M1/1‑128 (MQ=255)                 tCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttga                                                                                                                         >  1:603153‑M1/1‑125 (MQ=255)                         tacgacggcgaGCGACTTCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcgg                                                                                                                 <  1:422927‑M1/142‑27 (MQ=255)                         gacgacgacGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcgg                                                                                                                 <  1:499161‑M1/143‑27 (MQ=255)                          acgacgacgAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcgga                                                                                                                <  1:539816‑M1/143‑28 (MQ=255)                           cgacgacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaa                                                                                                               <  1:322788‑M1/143‑29 (MQ=255)                               gacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccctcttcttcgtggatgttgcggaggatc                                                                                                           >  1:489957‑M1/1‑111 (MQ=255)                                     cGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccg                                                                                                     <  1:296603‑M1/143‑39 (MQ=255)                                     cGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccg                                                                                                     >  2:308445‑M1/1‑105 (MQ=255)                                     cGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTATTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccg                                                                                                     <  2:83068‑M1/143‑39 (MQ=255)                                      gACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccgg                                                                                                    <  1:203362‑M1/143‑40 (MQ=255)                                          gcgcGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGGTTCTTTGAAGACGACGACGGCCTGCAActccttcttcttctttgaagatgcggaagatcacgccggtaac                                                                                                >  1:373678‑M1/1‑100 (MQ=255)                                                       gAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGACGACGACGACGGCCTGCATctccttcttataatttgaagatgcggaagatcacgccggtaaatccactgtagcat                                                                                   >  1:673684‑M1/1‑87 (MQ=255)                                                          cgtggCCAGAGCGTGGGATCCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGCAGACGACGACGGCCTGCATctccgtcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcattta                                                                                <  2:277830‑M1/141‑60 (MQ=255)                                                           ttGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttac                                                                               >  1:712598‑M1/1‑83 (MQ=255)                                                              gCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccat                                                                            >  1:624649‑M1/1‑80 (MQ=255)                                                               ccAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagctgcggaagatcacgacggcacctgcaatgtggcacttaccatc                                                                           >  2:556712‑M1/1‑79 (MQ=255)                                                                 agagCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGAACGATTCTTTGACGACGACGACGGCCTGCATcccgctgttcttctttgaagacgcggaagatgacggcggtaactccgctgtggcatgtacgatccg                                                                         >  1:99764‑M1/1‑77 (MQ=255)                                                                      cTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatg                                                                    >  2:455451‑M1/1‑72 (MQ=255)                                                                      cTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGAATctccttcttcttctttgaagatgcggaagatcacgcaggtaactccactgtggcatttaccatccgtgatg                                                                    >  1:619746‑M1/1‑72 (MQ=255)                                                                         gCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatgcgc                                                                 >  1:151269‑M1/1‑69 (MQ=255)                                                                                gcccgGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtt                                                          <  1:278320‑M1/143‑82 (MQ=255)                                                                                            aaGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtga                                              <  2:215353‑M1/143‑94 (MQ=255)                                                                                                   ccaagcATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggtatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaa                                       <  1:44431‑M1/141‑101 (MQ=255)                                                                                                                      tttGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgt                    >  1:541801‑M1/1‑24 (MQ=255)                                                                                                                         gAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatc                 <  1:455451‑M1/143‑123 (MQ=255)                                                                                                                                        cTGCATctccttcttcttctttgaagatgcggattttaccacccgtagctccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtgtgcgtgcccgta  >  2:87582‑M1/1‑6 (MQ=255)                                                                                                                                             |                                                                                                                                          GTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001504‑4001781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑