Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	32 [0]	[0] 32	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	32 (0.790)	27/284	0.5	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTA  >  NC_000913/4001509‑4001786                                                                                                                                              |                                                                                                                                         gtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgttactttgaagacgacgacggccggcatct                                                                                                                                         >  1:648021‑M2/142‑143 (MQ=255)           atcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttctt                                                                                                                               <  1:465128‑M2/12‑1 (MQ=255)            tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgagcttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                              >  1:678700‑M2/131‑143 (MQ=255)            tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagccttgcaacccgcgcggaactggaagacatcgaagcatgggcacggttatttcaagacgacgacggcctgcatCTCctgattcttc                                                                                                                              >  2:283421‑M2/131‑143 (MQ=255)            tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttc                                                                                                                              >  1:675534‑M2/131‑143 (MQ=255)              ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcgacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                            >  1:315507‑M2/129‑143 (MQ=255)                 tcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGa                                                                                                                         >  1:603153‑M2/126‑143 (MQ=255)                         tacgacggcgagcgacttcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCgg                                                                                                                 <  1:422927‑M2/26‑1 (MQ=255)                         gacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCgg                                                                                                                 <  1:499161‑M2/26‑1 (MQ=255)                          acgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGa                                                                                                                <  1:539816‑M2/27‑1 (MQ=255)                           cgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGaa                                                                                                               <  1:322788‑M2/28‑1 (MQ=255)                               gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCCTCTTCTTCGTGGATGTTGCGGAGGATc                                                                                                           >  1:489957‑M2/112‑143 (MQ=255)                                     cgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                     <  1:296603‑M2/38‑1 (MQ=255)                                     cgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                     >  2:308445‑M2/106‑143 (MQ=255)                                     cgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttatttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                     <  2:83068‑M2/38‑1 (MQ=255)                                      gactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCgg                                                                                                    <  1:203362‑M2/39‑1 (MQ=255)                                          gcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacggttctttgaagacgacgacggcctgcaaCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAAc                                                                                                >  1:373678‑M2/101‑143 (MQ=255)                                                       gaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcatCTCCTTCTTATAATTTGAAGATGCGGAAGATCACGCCGGTAAATCCACTGTAGCAt                                                                                   >  1:673684‑M2/88‑143 (MQ=255)                                                          cgtggccagagcgtgggatcccgcccggaactggaagacatcgaagcatcggcacgtttctttgcagacgacgacggcctgcatCTCCGTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTa                                                                                <  2:277830‑M2/59‑1 (MQ=255)                                                           ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                               >  1:712598‑M2/84‑143 (MQ=255)                                                              gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCAt                                                                            >  1:624649‑M2/81‑143 (MQ=255)                                                               ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGCTGCGGAAGATCACGACGGCACCTGCAATGTGGCACTTACCATc                                                                           >  2:556712‑M2/80‑143 (MQ=255)                                                                 agagcctggcaacccgcccggaactggaagacatcgaagcatcggaacgattctttgacgacgacgacggcctgcatCCCGCTGTTCTTCTTTGAAGACGCGGAAGATGACGGCGGTAACTCCGCTGTGGCATGTACGATCCg                                                                         >  1:99764‑M2/78‑143 (MQ=255)                                                                      ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                    >  2:455451‑M2/73‑143 (MQ=255)                                                                      ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgaatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCAGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                    >  1:619746‑M2/73‑143 (MQ=255)                                                                         gcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTgatgcgc                                                                 >  1:151269‑M2/70‑140 (MQ=255)                                                                                gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                          <  1:278320‑M2/81‑1 (MQ=255)                                                                                            aagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtga                                              <  2:215353‑M2/93‑1 (MQ=255)                                                                                                   ccaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGTATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                       <  1:44431‑M2/100‑1 (MQ=255)                                                                                                                      tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGt                    >  1:541801‑M2/25‑143 (MQ=255)                                                                                                                         gaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATc                 <  1:455451‑M2/122‑1 (MQ=255)                                                                                                                                        ctgcatCTCCTTCTTCTTCTTTGAAGATGCGGATTTTACCACCCGTAGCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTGTGCGTGCCCGTa  >  2:87582‑M2/7‑143 (MQ=255)                                                                                                                                              |                                                                                                                                         GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTA  >  NC_000913/4001509‑4001786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑