Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	36 [0]	[0] 36	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	35 (0.940)	32/276	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299363‑1299638                                                                                                                                        |                                                                                                                                             agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGGTACAGGAAAGTGCTAAataataa                                                                                                                                       >  2:93380‑M1/1‑136 (MQ=255)            aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                            <  2:481736‑M1/143‑19 (MQ=255)            aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                            <  1:515668‑M1/143‑19 (MQ=255)             ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAAGTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                           <  1:114059‑M1/143‑20 (MQ=255)                 ttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                       >  1:398291‑M1/1‑120 (MQ=255)                  tCCTTAACTGCTTCTCTTCATCATCATTTTATTTTCTTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                      <  1:234568‑M1/143‑25 (MQ=255)                    cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcacttgttaaattattgt                                                                                                                    <  2:615780‑M1/143‑27 (MQ=255)                     ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGGAAAGTGCTAAataataatcaattgttaaattaatgtg                                                                                                                   >  2:126913‑M1/1‑116 (MQ=255)                       aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                 >  2:526306‑M1/1‑114 (MQ=255)                          tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCGCTGCCTTTGGTAGAGAAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                              >  1:551518‑M1/1‑111 (MQ=255)                                  ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                      >  1:148155‑M1/1‑103 (MQ=255)                                   tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                     >  2:86582‑M1/1‑102 (MQ=255)                                       catgatTTTATTTTCGTCACATTATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                 <  2:90363‑M1/143‑46 (MQ=255)                                         tcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                               <  1:592481‑M1/143‑48 (MQ=255)                                            tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                            >  2:131927‑M1/1‑93 (MQ=255)                                             gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                           <  1:63403‑M1/143‑52 (MQ=255)                                                 ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAActaataatccattgttctattatggtgcatttcaattctgtaactgtaatcagaa                                                                                       >  1:59970‑M1/1‑88 (MQ=255)                                                   ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                     <  1:91790‑M1/143‑58 (MQ=255)                                                      cccaCATCATAATCCTGGGCTTGCTTAAGAATAATTTAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                  <  1:438021‑M1/142‑61 (MQ=255)                                                       ccACGTGATAATCCTTGCTTTGGTGAAGAATAATTGCAATGGTATTATTAATTCCACTGTCTTTTGTAGAGGAAAGTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                 <  2:587260‑M1/143‑62 (MQ=255)                                                         acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACAGCCAGTGGGAGAGCCAACTGCTAAataataatcaaccgttaaatcattgtccatttcactactggaactgcaatcagaaacgataga                                                                               >  2:118730‑M1/1‑80 (MQ=255)                                                             catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgcaactgtaatcagaaaagatagacaag                                                                           >  1:372318‑M1/1‑76 (MQ=255)                                                                          ttGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaagatagacatgcttagccaatcac                                                              >  2:346272‑M1/1‑63 (MQ=255)                                                                           tGCTGAAGAATAATTGAATTGATATTATTAATTACACTGCCTTTGGTAGAGGAAAGAGCTAAaaaataatcaattgttaaattatggtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                             <  1:120699‑M1/143‑82 (MQ=255)                                                                                  gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                      <  2:24151‑M1/143‑89 (MQ=255)                                                                                     taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatgagaaaagatagacatgcttagccaatctctattagattga                                                   >  1:57072‑M1/1‑52 (MQ=255)                                                                                                   ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                     <  1:131927‑M1/143‑106 (MQ=255)                                                                                                   ttagtaATTCCAAAGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                     <  2:667547‑M1/143‑106 (MQ=255)                                                                                                      ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagagaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                  >  1:609181‑M1/1‑35 (MQ=255)                                                                                                       tcattCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtta                                 <  2:531635‑M1/141‑110 (MQ=255)                                                                                                               ttgtctttgTTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                         <  1:46608‑M1/139‑118 (MQ=255)                                                                                                                         tAGATGAAAGTGCTAAatattaatcatttgttaaattattgtgcatttcactattggaactgtaattagaaaagatagacatgcttagtcaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               <  2:21352‑M1/143‑128 (MQ=255)                                                                                                                         tAGAGGAAAGCGGTAAataataatcaactgttaaattattgttcatttcactaatggaactgaaaccagaaaagatacaaatgtttatccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               <  1:332871‑M1/143‑128 (MQ=255)                                                                                                                          agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat              >  1:210289‑M1/1‑15 (MQ=255)                                                                                                                                    gCTAAataattatcaattgtaaaattattgtgcttttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat    <  1:93380‑M1/143‑139 (MQ=255)                                                                                                                                      tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggctgcaagctatagattctgatac  >  2:684565‑M1/1‑3 (MQ=255)                                                                                                                                        |                                                                                                                                             AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299363‑1299638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑