Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	36 [0]	[0] 36	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	35 (0.940)	32/276	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300562‑1300837                                                                                                                                         |                                                                                                                                            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactccctttggtacaggaaagtgctaaataataa                                                                                                                                       >  2:93380‑M2/137‑143 (MQ=255)            aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctggagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                            <  2:481736‑M2/18‑1 (MQ=255)            aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                            <  1:515668‑M2/18‑1 (MQ=255)             ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataagtgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                           <  1:114059‑M2/19‑1 (MQ=255)                 ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                       >  1:398291‑M2/121‑143 (MQ=255)                  tccttaactgcttctcttcatcatcattttattttcttcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                      <  1:234568‑M2/24‑1 (MQ=255)                    cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAATTATtgt                                                                                                                    <  2:615780‑M2/26‑1 (MQ=255)                     ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagactaattgaaatgatattattaattccactgcctttgctagaggaaagtgctaaATAATAATCAATTGTTAAATTAAtgtg                                                                                                                   >  2:126913‑M2/117‑143 (MQ=255)                       aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                 >  2:526306‑M2/115‑143 (MQ=255)                          tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccgctgcctttggtagagaaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                              >  1:551518‑M2/112‑143 (MQ=255)                                  ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                      >  1:148155‑M2/104‑143 (MQ=255)                                   tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                     >  2:86582‑M2/103‑143 (MQ=255)                                       catgattttattttcgtcacattataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                 <  2:90363‑M2/45‑1 (MQ=255)                                         tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                               <  1:592481‑M2/47‑1 (MQ=255)                                            tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                            >  2:131927‑M2/94‑143 (MQ=255)                                             gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                           <  1:63403‑M2/51‑1 (MQ=255)                                                 ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaCTAATAATCCATTGTTCTATTATGGTGCATTTCAATTCTGTAACTGTAATCAGaa                                                                                       >  1:59970‑M2/89‑143 (MQ=255)                                                   ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                     <  1:91790‑M2/57‑1 (MQ=255)                                                      cccacatcataatcctgggcttgcttaagaataatttaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                  <  1:438021‑M2/60‑1 (MQ=255)                                                       ccacgtgataatccttgctttggtgaagaataattgcaatggtattattaattccactgtcttttgtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                 <  2:587260‑M2/61‑1 (MQ=255)                                                         acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacagccagtgggagagccaactgctaaATAATAATCAACCGTTAAATCATTGTCCATTTCACTACTGGAACTGCAATCAGAAACGATAGa                                                                               >  2:118730‑M2/81‑143 (MQ=255)                                                             cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGCAACTGTAATCAGAAAAGATAGacaag                                                                           >  1:372318‑M2/77‑141 (MQ=255)                                                                          ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAAGATAGACATGCTTAGCCaatcac                                                              >  2:346272‑M2/64‑141 (MQ=255)                                                                           tgctgaagaataattgaattgatattattaattacactgcctttggtagaggaaagagctaaAAAATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                             <  1:120699‑M2/81‑1 (MQ=255)                                                                                  gaataattgaaatgatattattaattccactgccttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                      <  2:24151‑M2/88‑1 (MQ=255)                                                                                     taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATGAGAAAAGATAGACATGCTTAGCCAATCTCTATtagattga                                                   >  1:57072‑M2/53‑143 (MQ=255)                                                                                                   ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                     <  1:131927‑M2/105‑1 (MQ=255)                                                                                                   ttagtaattccaaagcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                     <  2:667547‑M2/105‑1 (MQ=255)                                                                                                      ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAGAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                  >  1:609181‑M2/36‑143 (MQ=255)                                                                                                       tcattccactgccttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTa                                 <  2:531635‑M2/109‑1 (MQ=255)                                                                                                               ttgtctttgttagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                         <  1:46608‑M2/117‑1 (MQ=255)                                                                                                                         tagatgaaagtgctaaATATTAATCATTTGTTAAATTATTGTGCATTTCACTATTGGAACTGTAATTAGAAAAGATAGACATGCTTAGTCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta               <  2:21352‑M2/127‑1 (MQ=255)                                                                                                                         tagaggaaagcggtaaATAATAATCAACTGTTAAATTATTGTTCATTTCACTAATGGAACTGAAACCAGAAAAGATACAAATGTTTATCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta               <  1:332871‑M2/127‑1 (MQ=255)                                                                                                                          agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat              >  1:210289‑M2/16‑143 (MQ=255)                                                                                                                                    gctaaATAATTATCAATTGTAAAATTATTGTGCTTTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt    <  1:93380‑M2/138‑1 (MQ=255)                                                                                                                                      taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGCTGCAAGCTATAGATTCTGATAc  >  2:684565‑M2/4‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300562‑1300837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑