Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,259,760	Δ1 bp :: IS186 (–) +5 bp	coding (1040‑1044/1680 nt)	dauA ←	C4‑dicarboxylic acid transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	608007 =	NA (NA)	55 (0.850)	50/276	0.2	100%	noncoding (1/1345 nt)	IS186	repeat region
	?	NC_000913	1259760 =	0 (0.000)					coding (1044/1680 nt)	dauA	C4‑dicarboxylic acid transporter
*	?	NC_000913	= 609350	NA (NA)	34 (0.520)	32/278	1.4	100%	noncoding (1344/1345 nt)	IS186	repeat region
	?	NC_000913	= 1259764	0 (0.000)					coding (1040/1680 nt)	dauA	C4‑dicarboxylic acid transporter

TCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/609207‑609350 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTGGAACATGAGTGAAGCGCA  <  NC_000913/1259764‑1259623                                                                                                                                                                                                                                                                                                tcgGGAGCTTACAGGCGGCCATCCGTGGGACAGTATCCCGGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGCGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                                                                                                 <  1:792620/140‑1          gACAGGTGGCCATTCGTTGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCC                                                                                                                                        <  1:327723/142‑1           atAGGTGGCCCTTCGTGGGACAGTATCCCTGTCATCCTACAAAACGCAATTGAAGAACGTGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCC                                                                                                                                       <  2:678363/141‑1           ACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCC                                                                                                                                       <  1:108567/143‑1             AGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCGACCGTGAAGCTAGCGCTTATGGGCAACGTCCCCT                                                                                                                                     >  1:809642/1‑143                      TTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCG                                                                                                                            >  1:767324/1‑143                         GTGGGACAGTATCCATGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGCCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTG                                                                                                                         >  1:556905/1‑143                          TGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGA                                                                                                                        >  1:920078/1‑143                              ACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTCAGCGCTTATGGGCAACGTCCCCTATCTCTGCGGTGATCCA                                                                                                                    >  2:334239/1‑143                                    TCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTATTCAGATGGTTCAACCCTCAAGTGAGCGCTTATGGGCAACGTCCTCTATCTCGGCGGTGATCTACTCTct                                                                                                              >  2:352299/1‑141                                        TGACACCCTACACAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCTACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTG                                                                                                          <  1:776206/143‑1                                              cgttcagAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTGAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATT                                                                                                    <  1:32578/136‑1                                                    AAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCC                                                                                              <  1:749891/143‑1                                                            TTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTAAGGTTATTCTTGCCATGCTGGT                                                                                      >  2:671116/1‑143                                                               AAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACT                                                                                   <  1:705917/143‑1                                                                       GAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGC                                                                           <  1:606542/143‑1                                                                           gATCGTCTGAACGCGGCGCCGTGGCCTCTCATTCTTCACATCGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCT                                                                       <  2:207875/142‑1                                                                                    AACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTAGCCCTGCTGGTACTGGCACCGCGGCTCTCCTGGCT                                                              >  2:934112/1‑143                                                                                    AACGAGGCACCGAGGCGTCGCATTCTTCAGATCGTTCAACCCTTAAGTTAGGGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCT                                                              >  2:628236/1‑143                                                                                      CGAGGCACCGAGGCGTCGCATTCTTGAGATGGTTCAACCCTCAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGC                                                            <  2:923054/143‑1                                                                                       GAGGCACCGATGCGTCGCATTCTTCAGATGGTACAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATACTTGTTATTCTTGCCCTGCTGGAACTGGCACAGCTACTCCCCTAGCTGCC                                                           >  2:310535/1‑143                                                                                          GCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCT                                                        >  1:1070046/1‑143                                                                                                     TCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGG                                             >  1:962302/1‑143                                                                                                       GCAATCTTCAGATGGTTCAACACTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTATATTCTGGTTATTATTGCCCTGCTGGTACTGGCACCGCTCCGCTCATGACTGCCGGTGTCCGCCATGGCA                                           >  2:545843/1‑143                                                                                                        CATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCcg                                          >  1:540708/1‑141                                                                                                                      cTCAACCCTTAAGTTAGCGCTGATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATG                            <  1:178989/142‑1                                                                                                                         AACCCTTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTG                         >  2:1180700/1‑143                                                                                                                          ACCCTTAAGTCAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCTCCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGG                        <  1:753915/143‑1                                                                                                                              TTAAGTTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTG                    >  1:1126741/1‑143                                                                                                                              TTAAGTTACCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTG                    <  1:889433/143‑1                                                                                                                                   TTAGCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTGGAACA               <  1:668976/143‑1                                                                                                                                    TAGCGCTTATGGGCAACGTCCCCTATCTCGCCGTTGCTCCACTCTCTTCTGGTTATTCTTGCCCTGATGCTACGGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTCGTCATCGCCGCGTGGAACAT              >  2:1085676/1‑143                                                                                                                                      GCGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTGGAACATGA            <  1:683998/143‑1                                                                                                                                       CGCTTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCCGTTGATGGTGGCGTGGCACcccag           >  2:1144261/1‑138                                                                                                                                          TTATGGGCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTCCCGCTTTCCGCCATGGCAGCCCTGCTGTTGCTGGCGGCGTGGAACACGAGTGc        >  2:1053452/1‑142                                                                                                                                                GCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTGGAACATGAGTGAAGCGCA  >  1:864182/1‑143                                                                                                                                                                                                                                                                                                TCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/609207‑609350 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCAACGTCCCCTATCTCGGCGGTGATCCACTCTATTCTGGTTATTCTTGCCCTGCTGGTACTGGCACCGCTGCTCTCCTGGCTGCCGCTTTCCGCCATGGCAGCCCTGCTGTTGATGGTGGCGTGGAACATGAGTGAAGCGCA  <  NC_000913/1259764‑1259623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.