Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,891 IS5 (–) +4 bp intergenic (+139/‑5) polA → / → spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)89 (1.390) 61/284 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4049894 0 (0.000)intergenic (+142/‑5) polA/spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 2290113NA (NA)71 (1.110) 59/284 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4049891 = 0 (0.000)intergenic (+139/‑8) polA/spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Only 100 of 161 total aligned reads displayed.

GATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCT  >  NC_000913/4049753‑4050036
                                                                                                                                             |                                                                                                                                              
gATGCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGACAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGg                                                                                                                                               >  1:597580‑M2/1‑142 (MQ=255)
    cGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaag                                                                                                                                           >  1:906193‑M2/1‑138 (MQ=255)
    agcCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaag                                                                                                                                           >  2:276452‑M2/2‑138 (MQ=255)
       cTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtg                                                                                                                                        <  1:767386‑M2/143‑9 (MQ=255)
        tttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAgg                                                                                                                                       <  1:830056‑M2/13‑1 (MQ=255)
          aaCATGTCTTTTTTCGTAAGTAAGCAACATAAGTTGTCATGTTTTGGGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcga                                                                                                                                     >  1:671744‑M2/1‑132 (MQ=255)
            tggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatggctcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGAGTGGGGTAc                                                                                                                                   >  2:524589‑M2/127‑143 (MQ=255)
              tGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgactaa                                                                                                                                 <  1:1002191‑M2/143‑16 (MQ=255)
               gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACTACTGAAAAAACATTACACAACGTGCTTTCTGAACTGAACAAAAAAGAGTACAGTTAAggaaagtgtggataag                                                                                                                                <  2:125423‑M2/143‑17 (MQ=255)
                ctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACagag                                                                                                                               >  1:861427‑M2/123‑143 (MQ=255)
                  tttttaCGTAAGTAAGAAACATAAGCTGTAACGTTTTGTAATGGCTATTAAAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcgg                                                                                                                             >  2:387310‑M2/1‑124 (MQ=255)
                    cgccgcttactgctgtattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatcgctccagatgacaaacatgagctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTaa                                                                                                                           <  1:654402‑M2/25‑1 (MQ=255)
                     ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAg                                                                                                                          <  2:783933‑M2/26‑1 (MQ=255)
                        gcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATg                                                                                                                       >  2:927814‑M2/115‑143 (MQ=255)
                         gtaagtaaGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAGTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTCAAGTTAGagaaaatacgaataagcggggaaatt                                                                                                                      <  1:309128‑M2/143‑27 (MQ=255)
                          ttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttggggtctgccATAGTCGCGTAGGGTACAGAGGTAAGATGtt                                                                                                                     >  1:481606‑M2/113‑143 (MQ=255)
                              taagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAGAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgcataagcgtggaaatgcttct                                                                                                                 <  1:460316‑M2/143‑32 (MQ=255)
                                 tgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatctcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTACTATCtt                                                                                                              >  2:73099‑M2/106‑143 (MQ=255)
                                 gCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaagaagcggggaaagtcttctcgg                                                                                                              <  2:616575‑M2/143‑35 (MQ=255)
                                  gaattcacggtcggcgaaggtaagttgatgactcattatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcacctttcTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCttt                                                                                                             <  2:541273‑M2/39‑1 (MQ=255)
                                   aattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTc                                                                                                            <  2:670817‑M2/40‑1 (MQ=255)
                                   aattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTc                                                                                                            >  1:64396‑M2/104‑143 (MQ=255)
                                   aaCATAAGAAGTAACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggct                                                                                                            >  2:1145254‑M2/1‑107 (MQ=255)
                                    attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCa                                                                                                           <  1:919616‑M2/41‑1 (MQ=255)
                                     ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAg                                                                                                          >  2:1043024‑M2/102‑143 (MQ=255)
                                     ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGAAGTACGATCTTTTAg                                                                                                          >  1:424759‑M2/102‑143 (MQ=255)
                                      aTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgac                                                                                                         <  2:816909‑M2/143‑40 (MQ=255)
                                        actgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGAcc                                                                                                       >  1:350962‑M2/99‑143 (MQ=255)
                                          tgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCtt                                                                                                     <  1:596946‑M2/47‑1 (MQ=255)
                                          tgtcggcgaaggtaagttgatgactcatgatgaacactgttctatggctccagatgacaaacataatctgatatcagggacttgttcgcaacttccTGAGTCGCGTAGGGTACGGACGTAAGATGGTGTATGTTTCAgaccat                                                                                                     >  2:637636‑M2/97‑141 (MQ=255)
                                          gCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcag                                                                                                     <  1:1117862‑M2/143‑44 (MQ=255)
                                           gtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                    >  2:174111‑M2/96‑143 (MQ=255)
                                            tGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtc                                                                                                   <  2:34847‑M2/143‑46 (MQ=255)
                                              ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTAc                                                                                                 >  2:758384‑M2/93‑143 (MQ=255)
                                                aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcattt                                                                                               <  1:1062626‑M2/143‑50 (MQ=255)
                                                     gtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCCCGt                                                                                          >  1:704742‑M2/86‑143 (MQ=255)
                                                       aagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTaa                                                                                        <  2:625199‑M2/60‑1 (MQ=255)
                                                         gttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATc                                                                                      <  1:927814‑M2/62‑1 (MQ=255)
                                                          aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgacgcagtcatttcatttcttca                                                                                     <  2:242184‑M2/143‑60 (MQ=255)
                                                           tgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCgg                                                                                    <  2:9572‑M2/64‑1 (MQ=255)
                                                              cTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtt                                                                                 <  2:425522‑M2/143‑64 (MQ=255)
                                                               tATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgttt                                                                                <  1:403537‑M2/143‑65 (MQ=255)
                                                                 ctcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTgg                                                                              <  1:462519‑M2/70‑1 (MQ=255)
                                                                  tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                    <  1:939789‑M2/48‑1 (MQ=255)
                                                                  tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGc                                                                             >  1:741565‑M2/73‑143 (MQ=255)
                                                                     aaaTTCCAATGCAACAACAGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccg                                                                          >  2:226422‑M2/1‑73 (MQ=255)
                                                                       aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgat                                                                        <  1:976832‑M2/143‑73 (MQ=255)
                                                                        tgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAata                                                                       >  1:757956‑M2/67‑143 (MQ=255)
                                                                            tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttttt                                                                   <  2:25694‑M2/143‑78 (MQ=255)
                                                                              ctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                                 <  1:1074989‑M2/83‑1 (MQ=255)
                                                                               tgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgc                                                                <  1:155291‑M2/84‑1 (MQ=255)
                                                                               tgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGCAGGGTACAGAGGTAGGATGTTCTCTCTTTCAGACCTTTTACTTCACGTAAGCGGATTGGGCTGAAAACATTAgc                                                                >  1:776449‑M2/60‑143 (MQ=255)
                                                                                caacaaCTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcc                                                               >  1:816909‑M2/1‑62 (MQ=255)
                                                                                 aacaacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctccc                                                              <  1:663535‑M2/143‑83 (MQ=255)
                                                                                   ctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgcc                                                            >  2:587282‑M2/56‑143 (MQ=255)
                                                                                     acTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaa                                                          <  2:643385‑M2/143‑87 (MQ=255)
                                                                                        gctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagt                                                       <  2:849550‑M2/93‑1 (MQ=255)
                                                                                         aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgcttttttctcccgtaaatgc                                                      <  1:333773‑M2/143‑91 (MQ=255)
                                                                                          tccagatgacgagcatgatctcatatcagggacttgtgcgcaccgtccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtca                                                     <  2:927173‑M2/95‑1 (MQ=255)
                                                                                           ccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGAACTTTGAGTTCACGTAATCGGATTTGGCTGAATATTTTAGGCAGCcccgtcat                                                    >  2:128373‑M2/48‑142 (MQ=255)
                                                                                            aataatGACGAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcctt                                                   >  2:228971‑M2/1‑50 (MQ=255)
                                                                                            aaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcctt                                                   >  1:425522‑M2/1‑50 (MQ=255)
                                                                                               aaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgttttagccgattttttctcccgtaaatgccttgaa                                                >  2:1134298‑M2/1‑47 (MQ=255)
                                                                                                aTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttattctcccgtaaatgccttgaat                                               >  2:767386‑M2/1‑46 (MQ=255)
                                                                                                   aaaaaatgtGCTTTCCGCACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcgggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagc                                            >  2:111281‑M2/3‑43 (MQ=255)
                                                                                                     aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatggcttgaatcagcc                                          <  1:1000358‑M2/143‑103 (MQ=255)
                                                                                                          gatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGt                                     >  1:650133‑M2/33‑143 (MQ=255)
                                                                                                          gTCCTTTAGGAACTGAACAAAAAAGAGTTAAGTTAGtgaatgtgcgaataagcggtgaaattcttctcggctaactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctattt                                     >  2:917968‑M2/1‑36 (MQ=255)
                                                                                                            tctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGCTGTTCTATCTTTCAGACCTTTTACTTCACGAAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCCGTAATGACTGGGGCGttt                                   >  2:389805‑M2/31‑143 (MQ=255)
                                                                                                             ctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGCATTTGGCGGCCTATTTTAGCCGCCACAGTCCGTTCTGACCGGGGCgtttg                                  >  1:509768‑M2/30‑142 (MQ=255)
                                                                                                             cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                                  >  2:1046483‑M2/1‑33 (MQ=255)
                                                                                                             cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                                  >  1:131318‑M2/1‑33 (MQ=255)
                                                                                                              tcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttttt                                 >  1:784460‑M2/29‑143 (MQ=255)
                                                                                                                    cagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCCTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                           <  1:948145‑M2/121‑1 (MQ=255)
                                                                                                                    aactgaacAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttc                           >  2:770990‑M2/1‑26 (MQ=255)
                                                                                                                       ttaacaaAAAAGAGTAAATTTAGggaaggtgcgaataagcggggaaattctgctcggctgacgcagtcatttcatttcttcatgtttgagccgatttcttctcccgtaaatgccttgaatcagcctatttagaccgtttcttc                        >  1:503613‑M2/3‑23 (MQ=255)
                                                                                                                             aaaaaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatt                  <  1:761367‑M2/143‑127 (MQ=255)
                                                                                                                              ttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaaa                 <  1:587282‑M2/131‑1 (MQ=255)
                                                                                                                              aaaaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccattt                 <  1:329883‑M2/143‑128 (MQ=255)
                                                                                                                               tcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                <  1:30019‑M2/132‑1 (MQ=255)
                                                                                                                               tcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGCTTTTTATTGGGCGaaagaaaa                >  1:11816‑M2/12‑143 (MQ=255)
                                                                                                                               tcgcaccttccGTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTGTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                <  2:851719‑M2/132‑1 (MQ=255)
                                                                                                                                aaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatccgcctatttagaccgtgtcttcgccatttaa               <  1:217780‑M2/143‑130 (MQ=255)
                                                                                                                                 agagTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaag              >  1:643385‑M2/1‑13 (MQ=255)
                                                                                                                                   accttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATc            >  1:242172‑M2/8‑143 (MQ=255)
                                                                                                                                    gTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcg           <  1:365256‑M2/143‑134 (MQ=255)
                                                                                                                                      ttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGt         <  2:807492‑M2/139‑1 (MQ=255)
                                                                                                                                       aaGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttcaggcgtta        <  2:706136‑M2/143‑137 (MQ=255)
                                                                                                                                        aGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttat       >  2:115224‑M2/1‑6 (MQ=255)
                                                                                                                                         gTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtcaatggcttgcatcagcctctttagactgtttcttcgccatttaaggcgttata      <  1:934475‑M2/143‑139 (MQ=255)
                                                                                                                                          ttAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatca     <  2:558805‑M2/143‑140 (MQ=255)
                                                                                                                                          ttAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgctagcc     <  1:911344‑M2/143‑140 (MQ=255)
                                                                                                                                          ttAGggaaggtgcgaacaagcggggaaatgcttctcggctgactcagtcatttcatttcttcatgtttgagccgatgttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatcc     >  1:237230‑M2/1‑4 (MQ=255)
                                                                                                                                          ttAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATg     >  2:1022651/1‑143 (MQ=255)
                                                                                                                                           tAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcgtgcttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatccc    >  2:760251‑M2/1‑3 (MQ=255)
                                                                                                                                            aGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatcccc   >  2:642388‑M2/1‑2 (MQ=255)
                                                                                                                                             gggaaggttcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatcccca  >  1:279306‑M2/1‑1 (MQ=255)
                                                                                                                                             gggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgcttttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttacggcgttataccca  <  2:521936‑M2/143‑143 (MQ=255)
                                                                                                                                             gggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatcccca  <  1:178525‑M2/143‑143 (MQ=255)
                                                                                                                                             gggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcccatttagaccgtttcttcccgatttaaggcgggatcccca  <  2:363817‑M2/143‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCT  >  NC_000913/4049753‑4050036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.