Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,891 IS5 (–) +4 bp intergenic (+139/‑5) polA → / → spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)89 (1.390) 61/284 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4049894 0 (0.000)intergenic (+142/‑5) polA/spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 2290113NA (NA)71 (1.110) 59/284 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4049891 = 0 (0.000)intergenic (+139/‑8) polA/spf 5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease/Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Only 100 of 151 total aligned reads displayed.

CTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATG  >  NC_000913/4049749‑4050033
                                                                                                                                              |                                                                                                                                              
cTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCGGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGt                                                                                                                                                <  2:298506/143‑1 (MQ=255)
  aaGATTCGCCTGAACATGCCTTGTTTCGTAAGCAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTa                                                                                                                                              <  2:269083/143‑1 (MQ=255)
    gATGCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGACAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGg                                                                                                                                            >  1:597580‑M2/1‑142 (MQ=255)
        cGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaag                                                                                                                                        >  1:906193‑M2/1‑138 (MQ=255)
        agcCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaag                                                                                                                                        >  2:276452‑M2/2‑138 (MQ=255)
           cTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtg                                                                                                                                     <  1:767386‑M2/143‑9 (MQ=255)
           cTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtg                                                                                                                                     <  1:665838‑M2/143‑9 (MQ=255)
            tttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAgg                                                                                                                                    <  1:830056‑M2/13‑1 (MQ=255)
            tgtctgggctgacggcgctgactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAgg                                                                                                                                    <  1:141427‑M2/13‑1 (MQ=255)
            tGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgc                                                                                                                                    >  2:116003‑M2/1‑134 (MQ=255)
              aaCATGTCTTTTTTCGTAAGTAAGCAACATAAGTTGTCATGTTTTGGGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcga                                                                                                                                  >  1:671744‑M2/1‑132 (MQ=255)
                tggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatggctcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGAGTGGGGTAc                                                                                                                                >  2:524589‑M2/127‑143 (MQ=255)
                 aTGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaata                                                                                                                               >  2:1043529‑M2/1‑129 (MQ=255)
                  tGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgactaa                                                                                                                              <  1:1002191‑M2/143‑16 (MQ=255)
                   gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACTACTGAAAAAACATTACACAACGTGCTTTCTGAACTGAACAAAAAAGAGTACAGTTAAggaaagtgtggataag                                                                                                                             <  2:125423‑M2/143‑17 (MQ=255)
                       acggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTa                                                                                                                         <  1:593950‑M2/24‑1 (MQ=255)
                        cgccgcttactgctgtattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatcgctccagatgacaaacatgagctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTaa                                                                                                                        <  1:654402‑M2/25‑1 (MQ=255)
                         ggcgcttcctgctcaattcattgtcggcgaaggtaagttgattactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAg                                                                                                                       <  1:442883‑M2/26‑1 (MQ=255)
                         ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAg                                                                                                                       <  2:783933‑M2/26‑1 (MQ=255)
                           cgctgactgctgaattcactgtcggcgaatgtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGAt                                                                                                                     <  1:859498‑M2/28‑1 (MQ=255)
                            gcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATg                                                                                                                    >  2:927814‑M2/115‑143 (MQ=255)
                             gtaagtaaGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAGTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTCAAGTTAGagaaaatacgaataagcggggaaatt                                                                                                                   <  1:309128‑M2/143‑27 (MQ=255)
                              ttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttggggtctgccATAGTCGCGTAGGGTACAGAGGTAAGATGtt                                                                                                                  >  1:481606‑M2/113‑143 (MQ=255)
                                  taagCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAGAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgcataagcgtggaaatgcttct                                                                                                              <  1:460316‑M2/143‑32 (MQ=255)
                                     gCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaagaagcggggaaagtcttctcgg                                                                                                           <  2:616575‑M2/143‑35 (MQ=255)
                                      gaattcacggtcggcgaaggtaagttgatgactcattatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcacctttcTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCttt                                                                                                          <  2:541273‑M2/39‑1 (MQ=255)
                                       aattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTc                                                                                                         >  1:64396‑M2/104‑143 (MQ=255)
                                       aaCATAAGAAGTAACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggct                                                                                                         >  2:1145254‑M2/1‑107 (MQ=255)
                                        attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCa                                                                                                        <  1:919616‑M2/41‑1 (MQ=255)
                                        attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGAACAGAGGTAAGATGTTCTATCTTTCa                                                                                                        >  2:916478‑M2/103‑143 (MQ=255)
                                         ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAg                                                                                                       >  2:1043024‑M2/102‑143 (MQ=255)
                                         ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGAAGTACGATCTTTTAg                                                                                                       >  1:424759‑M2/102‑143 (MQ=255)
                                          aTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgac                                                                                                      <  2:816909‑M2/143‑40 (MQ=255)
                                            actgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGAcc                                                                                                    >  1:350962‑M2/99‑143 (MQ=255)
                                              tgtcggcgaaggtaagttgatgactcatgatgaacactgttctatggctccagatgacaaacataatctgatatcagggacttgttcgcaacttccTGAGTCGCGTAGGGTACGGACGTAAGATGGTGTATGTTTCAgaccat                                                                                                  >  2:637636‑M2/97‑141 (MQ=255)
                                              gCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcag                                                                                                  <  1:1117862‑M2/143‑44 (MQ=255)
                                               gtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                 >  2:174111‑M2/96‑143 (MQ=255)
                                                tGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtc                                                                                                <  2:34847‑M2/143‑46 (MQ=255)
                                                 cggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagattacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTa                                                                                               <  2:627175‑M2/50‑1 (MQ=255)
                                                  tCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcat                                                                                              <  1:115224‑M2/143‑48 (MQ=255)
                                                  ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTAc                                                                                              >  2:758384‑M2/93‑143 (MQ=255)
                                                    aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcattt                                                                                            <  1:1062626‑M2/143‑50 (MQ=255)
                                                              aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttca                                                                                  <  2:379478‑M2/143‑60 (MQ=255)
                                                               tgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCgg                                                                                 <  2:9572‑M2/64‑1 (MQ=255)
                                                                 gCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgt                                                                               <  2:663411‑M2/143‑63 (MQ=255)
                                                                    aTTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttg                                                                            >  1:916286‑M2/1‑78 (MQ=255)
                                                                     ctcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTgg                                                                           <  1:462519‑M2/70‑1 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                 <  1:939789‑M2/48‑1 (MQ=255)
                                                                      tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGc                                                                          >  1:741565‑M2/73‑143 (MQ=255)
                                                                        atgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTCGGCTg                                                                        >  1:65423‑M2/71‑143 (MQ=255)
                                                                          gatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGaa                                                                      <  1:934557‑M2/75‑1 (MQ=255)
                                                                           aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgat                                                                     <  1:976832‑M2/143‑73 (MQ=255)
                                                                           aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgcttcagccgat                                                                     <  2:736694‑M2/143‑73 (MQ=255)
                                                                            tgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAata                                                                    >  1:757956‑M2/67‑143 (MQ=255)
                                                                                tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttttt                                                                <  2:25694‑M2/143‑78 (MQ=255)
                                                                                ccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAtttt                                                                >  2:845843‑M2/63‑143 (MQ=255)
                                                                                  tGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttct                                                              <  1:950347‑M2/143‑80 (MQ=255)
                                                                                  ctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                              <  1:900754‑M2/83‑1 (MQ=255)
                                                                                  ctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAg                                                              <  1:1074989‑M2/83‑1 (MQ=255)
                                                                                       ctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgcc                                                         >  2:587282‑M2/56‑143 (MQ=255)
                                                                                         acTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaa                                                       <  2:643385‑M2/143‑87 (MQ=255)
                                                                                            gctccagatgacaaacatgatctcatatcagtgacttgttcgcactttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagt                                                    <  1:930463‑M2/93‑1 (MQ=255)
                                                                                             aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgcttttttctcccgtaaatgc                                                   <  1:333773‑M2/143‑91 (MQ=255)
                                                                                             aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGATAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgc                                                   <  2:444384‑M2/143‑91 (MQ=255)
                                                                                              tccagatgacgagcatgatctcatatcagggacttgtgcgcaccgtccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtca                                                  <  2:927173‑M2/95‑1 (MQ=255)
                                                                                              tccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtca                                                  >  2:425412‑M2/49‑143 (MQ=255)
                                                                                                aataatGACGAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcctt                                                >  2:228971‑M2/1‑50 (MQ=255)
                                                                                                  aaaGTACAAAAAGGCCTTTCCGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttga                                              >  1:190053‑M2/1‑48 (MQ=255)
                                                                                                   atgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAt                                             <  2:235672‑M2/100‑1 (MQ=255)
                                                                                                    aTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgatttattctcccgtaaatgccttgaat                                            >  2:767386‑M2/1‑46 (MQ=255)
                                                                                                       aaaaaatgtGCTTTCCGCACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcgggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagc                                         >  2:111281‑M2/3‑43 (MQ=255)
                                                                                                       aCAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcag                                         >  2:161186‑M2/1‑43 (MQ=255)
                                                                                                         aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatggcttgaatcagcc                                       <  1:1000358‑M2/143‑103 (MQ=255)
                                                                                                          acatgatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTggg                                      <  2:807403‑M2/107‑1 (MQ=255)
                                                                                                           aaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagccta                                     <  2:1149558‑M2/143‑105 (MQ=255)
                                                                                                              gatctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCAGATTTGGCTTAATATTTTAGCCGCCCCAGTCAGTAATCACTGGGGCGt                                  >  2:1123617‑M2/33‑143 (MQ=255)
                                                                                                                tctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGCTGTTCTATCTTTCAGACCTTTTACTTCACGAAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCCGTAATGACTGGGGCGttt                                >  2:389805‑M2/31‑143 (MQ=255)
                                                                                                                 ctcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGCATTTGGCGGCCTATTTTAGCCGCCACAGTCCGTTCTGACCGGGGCgtttg                               >  1:509768‑M2/30‑142 (MQ=255)
                                                                                                                 cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttaga                               >  1:131318‑M2/1‑33 (MQ=255)
                                                                                                                  tcatatcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttttt                              >  1:784460‑M2/29‑143 (MQ=255)
                                                                                                                       tcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                         >  1:807492‑M2/24‑143 (MQ=255)
                                                                                                                       tcagggacttgttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                         <  1:624123‑M2/120‑1 (MQ=255)
                                                                                                                       gaactgaacAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgttt                         <  1:726878‑M2/143‑117 (MQ=255)
                                                                                                                        ccgggccttgttggcagcttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                        <  1:423883‑M2/121‑1 (MQ=255)
                                                                                                                        aactgaacAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcaggctatttagaccgtttc                        <  1:774657‑M2/143‑118 (MQ=255)
                                                                                                                        aactgaacAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttc                        >  2:770990‑M2/1‑26 (MQ=255)
                                                                                                                           ttaacaaAAAAGAGTAAATTTAGggaaggtgcgaataagcggggaaattctgctcggctgacgcagtcatttcatttcttcatgtttgagccgatttcttctcccgtaaatgccttgaatcagcctatttagaccgtttcttc                     >  1:503613‑M2/3‑23 (MQ=255)
                                                                                                                             aacAAAAAAGAGTAAAGTTAGggaaggtgcgaataagcggggacattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattctttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgc                   >  2:714446‑M2/1‑21 (MQ=255)
                                                                                                                                 aaaaaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatt               <  1:761367‑M2/143‑127 (MQ=255)
                                                                                                                                  ttcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaagaaa              <  1:587282‑M2/131‑1 (MQ=255)
                                                                                                                                  aaaaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccattt              <  1:329883‑M2/143‑128 (MQ=255)
                                                                                                                                   tcgcaccttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa             <  1:30019‑M2/132‑1 (MQ=255)
                                                                                                                                   tcgcaccttccGTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTGTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa             <  2:851719‑M2/132‑1 (MQ=255)
                                                                                                                                    aaGAGTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatccgcctatttagaccgtgtcttcgccatttaa            <  1:217780‑M2/143‑130 (MQ=255)
                                                                                                                                       accttccTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATc         >  1:242172‑M2/8‑143 (MQ=255)
                                                                                                                                        gTAAAGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcg        <  1:365256‑M2/143‑134 (MQ=255)
                                                                                                                                           aaGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttcaggcgtta     <  2:706136‑M2/143‑137 (MQ=255)
                                                                                                                                           aaGTTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgtta     <  1:648296‑M2/143‑137 (MQ=255)
                                                                                                                                              ttAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatca  <  2:558805‑M2/143‑140 (MQ=255)
                                                                                                                                              ttAGggaaggtgcgaacaagcggggaaatgcttctcggctgactcagtcatttcatttcttcatgtttgagccgatgttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatcc  >  1:237230‑M2/1‑4 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATG  >  NC_000913/4049749‑4050033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.