Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,553,103 IS5 (+) +4 bp coding (19‑22/2706 nt) malT → mal regulon transcriptional activator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)47 (0.960) 36/282 0.3 95.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3553103 = 2 (0.040)coding (19/2706 nt) malT mal regulon transcriptional activator
* ? NC_000913 = 2290113NA (NA)69 (1.420) 41/280 0.2 98.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3553106 1 (0.020)coding (22/2706 nt) malT mal regulon transcriptional activator

TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCG  >  NC_000913/2288778‑2289051
                                                                                                                                             |                                                                                                                                    
ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGACTATaagg                                                                                                                                     >  1:55704/1‑143 (MQ=255)
           gTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGCAGGTGCGAAt                                                                                                                          >  2:207063/1‑143 (MQ=255)
            ttCTCCGCCAGGATTGATTTTCGTATTATACGCGGTTCGATGAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATa                                                                                                                         >  1:846292/1‑143 (MQ=255)
              ctcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCAATAAAACAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAg                                                                                                                       >  1:608630/1‑143 (MQ=255)
                 cGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCgg                                                                                                                    <  1:393489/143‑1 (MQ=255)
                             ttttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATtcttct                                                                                                        <  1:308385/143‑1 (MQ=255)
                               ttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg                                                                                                      >  2:736441/1‑143 (MQ=255)
                                   tattatTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGCGCGAATAAGCGGGGAAATTCTTCTCGGCTg                                                                                                  >  1:140947/1‑143 (MQ=255)
                                        ttGGCGGTCCCATGAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGCGTGAATATAAGGAAGGTGCGAATAAGCGCGGCACATCTTCTCGGCTGACTCa                                                                                             >  2:295515/1‑143 (MQ=255)
                                        ttGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                             <  2:311968/143‑1 (MQ=255)
                                                              ggATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                       <  1:61120/143‑1 (MQ=255)
                                                                atatCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                     <  2:683959/143‑1 (MQ=255)
                                                                atatCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACGCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGTGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                     <  2:132541/143‑1 (MQ=255)
                                                                atatCTTTCTCCAGGACAACACCATCAACGGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                     <  1:101681/143‑1 (MQ=255)
                                                                agatCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                     <  1:620318/141‑1 (MQ=255)
                                                                  atCTTTTTCCAGGACAACACCATCATCTGTCTCAACACCCGCAACAGTGGATGCCCACGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                   <  1:29639/143‑1 (MQ=21)
                                                                  atCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTTTCTCCTTACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                   <  1:609915/143‑1 (MQ=255)
                                                                    cttgtaccAGGAAAACACCACCAACTGTCCCACCATCCACAACAGGTGGGCCAAATGGTATGGGTGAATCTAAGGAAGGTGCGAATAAGCTGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                                                                 <  2:819172/137‑1 (MQ=17)
                                                                      ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGTCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCGGAt                                                               >  1:356837/1‑143 (MQ=255)
                                                                      ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                               >  1:69010/1‑143 (MQ=255)
                                                                         tCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                            >  1:375359/1‑143 (MQ=255)
                                                                          ccAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                           >  1:726094/1‑143 (MQ=255)
                                                                          ccAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                           <  1:401226/143‑1 (MQ=255)
                                                                                   cacCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCCTCATGTTTGAGCCGATCTTTTCTCCCTTa                                                  >  2:1897/1‑143 (MQ=255)
                                                                                     ccATCAACTGTCGCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                <  1:840797/143‑1 (MQ=255)
                                                                                       aTCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCGTGTTTGAGCCGATTTTTTCTCCCGtccatg                                              >  1:694672/1‑138 (MQ=255)
                                                                                          aaCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                           <  1:428932/143‑1 (MQ=255)
                                                                                             tGTCTCACCATACTCCACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTTCGAATAAGCGGGGAAATGCTTCGCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                        <  2:427364/143‑1 (MQ=255)
                                                                                                 tcACCATACTCAACAGTTGATGCGAAGGCTATGGGGGAATATAAGGCAGGTGGGAATAAGAGGGGAAATAATTCGCGGCTGACTAAGTCAATGAATTTCTTCAGGTTTGAGCCGAGTTTTTCGCCCGTAAAGGCCttaagtcg                                    >  2:198952/1‑137 (MQ=255)
                                                                                                 ccACCATACTCCACAGCTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                    <  1:657907/142‑1 (MQ=255)
                                                                                                   aCCATACTCAACAGTTGATACCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                  >  1:666251/1‑143 (MQ=255)
                                                                                                    ccATACTCAACAGGTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGcg                                 >  1:255015/1‑142 (MQ=255)
                                                                                                      aTACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTa                               >  2:613293/1‑143 (MQ=255)
                                                                                                        aCTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                             <  2:568215/143‑1 (MQ=255)
                                                                                                          tCAACAGGTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                           <  1:871546/143‑1 (MQ=255)
                                                                                                            aaCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                         <  1:18123/143‑1 (MQ=255)
                                                                                                             aCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                        <  2:475054/143‑1 (MQ=255)
                                                                                                             aCAGTTGATGCCAATGATATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                        <  1:234082/143‑1 (MQ=255)
                                                                                                              cAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCAGCCTATTTAGAcc                       >  2:458598/1‑143 (MQ=255)
                                                                                                                          aTGCTATGGGTGAATAGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCa           <  2:493970/143‑1 (MQ=255)
                                                                                                                             cTATGGGTGAATATAAGGAAGGTGCGAATAAGCGTGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt        <  2:608630/143‑1 (MQ=255)
                                                                                                                                  ggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc   <  1:661518/143‑1 (MQ=35)
                                                                                                                                   gggattataAGGAAGTTGCTAATAGCCGGTGAAATTGTTCTCGGCTGGCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg  <  2:665349/138‑1 (MQ=11)
                                                                                                                                             |                                                                                                                                    
TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCG  >  NC_000913/2288778‑2289051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.