New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3146750 =45 (0.920)3 (0.070) 3/260 7.1 7.5% intergenic (‑13/+106) yghW/yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
?NC_000913 3146776 = 33 (0.730)intergenic (‑39/+80) yghW/yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTC  >  NC_000913/3146609‑3146889
                                                                                                                                             |                                                                                                                                           
cATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTCATCCCGCCATTAAGCCTTTACCAACATGGTTATTCATCGTTCACCTCGTTc                                                                                                                                            >  2:501465/1‑143 (MQ=255)
 aTCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTca                                                                                                                                           >  1:664127/1‑142 (MQ=255)
  tCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCTTTAAACCTTTCCCAACATCGTTATTTATCGTTCACCTCCTTTGc                                                                                                                                          >  2:24790/1‑143 (MQ=255)
    cGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCta                                                                                                                                        <  1:758240/143‑1 (MQ=255)
      gtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTCGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCtata                                                                                                                                      >  2:293170/1‑143 (MQ=255)
                ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTCTATAGTGTCata                                                                                                                            >  2:336228/1‑143 (MQ=255)
                     tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAg                                                                                                                       >  2:556395/1‑143 (MQ=255)
                      ccataaCCGCGTTTATAATCGGCACAGACTTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                      <  1:857529/139‑1 (MQ=255)
                      aCAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGGGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                      <  2:598782/143‑1 (MQ=255)
                      aCAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                      >  1:797308/1‑143 (MQ=255)
                      aCAAAACCGCGTTTAGAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                      <  2:599971/143‑1 (MQ=255)
                       cAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTCCCGAAATGGTTATTCATCGTTCACCTCATTCGCTCTATATAGTCATCTATcgcg                                                                                                                     >  2:493559/1‑143 (MQ=255)
                         aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGat                                                                                                                   >  1:330490/1‑143 (MQ=255)
                          aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGata                                                                                                                  >  1:492357/1‑143 (MQ=255)
                           aCCGCGTCTATAATCGGCACAGAATTTTGTCCCATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAACATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGatat                                                                                                                 <  1:606812/143‑1 (MQ=255)
                            ccGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtt                                                                                                                >  1:622979/1‑143 (MQ=255)
                             gccgTCTATGATCGGCCGAGAATTTTTTCACATTCACGTCACTTTCGTCCTGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCATAATGGTTATTCAGCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatgag                                                                                                               <  2:87454/141‑6 (MQ=255)
                              gcgTTTATAATCGGAACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatgagg                                                                                                              <  1:84698/143‑7 (MQ=255)
                                gTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                            <  2:314899/143‑1 (MQ=255)
                                    ataATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGGTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCaa                                                                                                        >  1:850037/1‑143 (MQ=255)
                                    acaaTCGGCACAGAATTTTGTCACATTAACCGCCCTGTCGGCAGGCTTTGCTTTTAATCCCGACAAAAGGGCTGTACCAAAAAGGTTATTCATCGGTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCaa                                                                                                        <  1:509214/141‑1 (MQ=255)
                                      aaTCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATCTATAGCGATATTTTAGGCAAAg                                                                                                      >  2:797165/1‑143 (MQ=255)
                                        tCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGaa                                                                                                    >  2:291398/1‑143 (MQ=255)
                                          ggCACAGTATTTTGTCACATTAACCGGACTGCCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACg                                                                                                  <  2:607815/143‑1 (MQ=255)
                                                       gTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACg                                                                                     <  1:293170/143‑1 (MQ=255)
                                                        tCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGc                                                                                    >  1:109551/1‑143 (MQ=255)
                                                          acaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTa                                                                                  >  2:837654/1‑143 (MQ=255)
                                                                    cccTGTCGTCATGCGTTGCTTTTAATCCCCCCATTAACCCTGTGCCAACATGGTTATTCATCGTTCACCTCACTCGCTATATATTGTCATATATCGCGATATTTTAGGCAACGCACGACTTTTGCGCCCGCAATTTTTCCaca                                                                        >  2:318230/3‑141 (MQ=255)
                                                                    cACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATg                                                                        >  1:760071/1‑143 (MQ=255)
                                                                        gTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTg                                                                    <  1:137335/143‑1 (MQ=255)
                                                                                       ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCCTATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTACATGCTTGGTTAGGACGTAATcg                                                     >  1:118330/1‑142 (MQ=255)
                                                                                       ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTCGTTTAAGACGTAATcc                                                     >  2:129013/1‑143 (MQ=255)
                                                                                         ttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCg                                                   <  1:634532/143‑1 (MQ=255)
                                                                                             tCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAag                                               >  1:694019/1‑143 (MQ=255)
                                                                                                cGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATCTTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGCCGTAATCCCGTAAgcga                                            >  2:41422/1‑143 (MQ=255)
                                                                                                cGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAgaga                                            >  1:362950/1‑143 (MQ=255)
                                                                                                         gCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTAt                                   <  2:507452/143‑1 (MQ=255)
                                                                                                               aCCAGAATGGTTATTGATCGTTCACCTCATTCGCTAGATATTGGCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCGTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGgag                             <  2:166067/143‑1 (MQ=255)
                                                                                                                 cAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGAGAAACCTATCAGgagag                           >  2:495766/1‑143 (MQ=255)
                                                                                                                  aaaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACAACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCGGTAACAGATAAACCTAACAGgagaac                          >  2:221479/1‑141 (MQ=255)
                                                                                                                             tCATCGTTCACCTCATTCGCTATATATCGTCCCCTCTACCTGTGGTTTTGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGaa               <  1:369890/143‑1 (MQ=255)
                                                                                                                              cATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAc              <  2:219999/143‑1 (MQ=255)
                                                                                                                                      aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTaa                                                               <  2:520096/86‑1 (MQ=255)
                                                                                                                                      aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTaa                                                               >  1:520096/1‑86 (MQ=255)
                                                                                                                                          cATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTc  <  2:376579/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
CATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTC  >  NC_000913/3146609‑3146889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.