Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	75 (1.540)	52/282	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	41 (0.840)	32/282	0.6	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  >  NC_000913/2101607‑2101885                                                                                                                                               |                                                                                                                                         tCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaag                                                                                                                                          <  2:408785/143‑1 (MQ=255)  cTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAGTCAACAACCGTCTCAGAGTCGCTCCTTTCTTTGGGATTTTTTATTCTATAaaga                                                                                                                                         >  1:34637/1‑142 (MQ=255)    tCACAGGACATAAAGTAAGCTTGAGCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaa                                                                                                                                       <  2:687309/143‑1 (MQ=255)     cacaGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaag                                                                                                                                      <  2:626843/143‑1 (MQ=255)          tACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCg                                                                                                                                 >  1:511203/1‑143 (MQ=255)            cATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGaa                                                                                                                               <  1:286938/143‑1 (MQ=255)              tAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATa                                                                                                                             <  1:703639/143‑1 (MQ=255)                 aGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGc                                                                                                                          <  2:467290/143‑1 (MQ=255)                 aGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGc                                                                                                                          <  2:470093/143‑1 (MQ=255)                   tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  2:235256/143‑1 (MQ=255)                   tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  1:419751/143‑1 (MQ=255)                   tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  1:419752/143‑1 (MQ=255)                   tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  2:226747/143‑1 (MQ=255)                   tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  2:126098/143‑1 (MQ=255)                     aCCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgggg                                                                                                                      <  2:23699/143‑1 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGATTTAGCggggta                                                                                                                    >  2:251619/1‑141 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGACTAAGCGGGGaa                                                                                                                    >  1:806098/1‑143 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAgtatggaa                                                                                                                    >  2:746008/1‑136 (MQ=255)                          gATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTCTTATTGCGGAAGGTGCGAATAAGCGTGGAGAtc                                                                                                                 >  2:128282/1‑142 (MQ=255)                             cGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttctt                                                                                                              >  1:177375/1‑143 (MQ=255)                             cGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttctt                                                                                                              >  2:801484/1‑143 (MQ=255)                                aGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGGGTTTTTGTATTCTTAAGGCCCGCGTGTATAAGAGGGGAAATTGTtgtc                                                                                                           >  1:120156/1‑143 (MQ=255)                                 gCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg                                                                                                          <  1:706143/143‑1 (MQ=255)                                  ccATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGTGAATAAGCGGGGAAATTCTTCTTgg                                                                                                         >  2:324843/1‑143 (MQ=255)                                  ccATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                         <  2:873756/143‑1 (MQ=255)                                    aTTTACACGCACAACATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                       <  2:249128/143‑1 (MQ=255)                                    aTTTACACGCACAAAATCTGAAAAACGAACAAGCATAAATGAGGCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTCTGGGAATTTTTTCTTTATACAGGAAGTTGCGCATAGTCAGGAACATTCTTCTCTGCt                                                                                                       >  2:81402/1‑143 (MQ=255)                                                       gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                    >  1:52226/1‑143 (MQ=255)                                                        aaaaaCGTACCAGCATAAATGATCCTGCGCACCAACCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                                                                   <  1:682760/143‑1 (MQ=255)                                                          aaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                 >  2:494599/1‑143 (MQ=255)                                                             cggaCCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                              <  1:386920/140‑1 (MQ=255)                                                               tACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGACTTTTTATTTTCTTACGGAAGCTGCGAATAAGCGGGGCAATTGTTCTTGGTTGACTCACTCACTCGCTTTCTTTATGtt                                                                            >  2:831294/1‑143 (MQ=255)                                                                  cAGCATAAATGAGCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                         <  1:393060/143‑1 (MQ=255)                                                                   aGCATAAATGATCCTGCGCACCAATCACCACCCGTATCCGAATAGTAACTTTCTTTAGGACTTTTTGTCTTATTCATGAAGGTGCGAATAAAACAGCTAGTTTTCCTCGTCTGCCTCTGTAATCTCATCACTTCATCTTTGAg                                                                        >  2:390519/1‑143 (MQ=255)                                                                   aGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                        <  2:177375/143‑1 (MQ=255)                                                                      tgaaaTGATCCTGCGCACCAATCAACACCCGTATCAGAATAGGTACTGTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                                                                     <  2:593116/141‑1 (MQ=255)                                                                      aTAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGCGCCg                                                                     >  2:146469/1‑143 (MQ=255)                                                                         aaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTGTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtt                                                                  >  1:287030/1‑143 (MQ=255)                                                                         aaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGAGCCGCtt                                                                  >  2:664267/1‑143 (MQ=255)                                                                          aTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttt                                                                 >  1:658804/1‑143 (MQ=255)                                                                           tGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTGGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGCTTGACCCTAtttt                                                                >  2:788674/1‑143 (MQ=255)                                                                             aTCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTGATGTTTGAGCCGAtggttt                                                              >  1:135616/1‑138 (MQ=255)                                                                                               cagccGTAGCAGAATAGATACTTTGTTGAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                            <  2:232394/140‑1 (MQ=255)                                                                                                       tCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGTTGACTCAGTTATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCACGTATTTGCCTTGAATccgtct                                    >  2:750771/1‑138 (MQ=18)                                                                                                        cAGAATAGAGACTGTCTGGAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTa                                   <  1:83447/143‑1 (MQ=255)                                                                                                          gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                                 <  2:491927/143‑1 (MQ=255)                                                                                                            aTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                               <  2:658804/143‑1 (MQ=255)                                                                                                                aTACTTTCTTTAGGAATTTTTGTTTTGTTAATGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGCCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCTGATTTTTTCTCCCGTCACTGCCTTCATTCAGACCATTTAGAcc                           >  2:161088/1‑143 (MQ=17)                                                                                                                    tttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGttt                       >  1:566473/1‑143 (MQ=255)                                                                                                                    tttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTACTTAGACCGttt                       >  2:252146/1‑143 (MQ=255)                                                                                                                    tttctttAGGAATTTTTGTTTTATTAAGGAAAAAGCGAGTAAGGGGGGAAATTCTTCTCGGCTGACTCAGTCGTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCCTTGATTGAGCCGATTTAGActtttt                       >  2:397289/1‑138 (MQ=17)                                                                                                                        tttAGGAATTTTTGTTTTATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                   <  1:452401/143‑1 (MQ=255)                                                                                                                              caTTGTTGTTTTATTAAGGAAGGTTCGAATAAGCTGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAtt             <  1:717532/142‑1 (MQ=255)                                                                                                                              aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTAATGTTTGAGCCGATTTTTTCTCCCGTAAATGCTTTGAATCAGCCTATTTAGACCGTTTCTTCTCCAtt             >  2:819486/1‑143 (MQ=255)                                                                                                                               aTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTATATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt            >  1:400484/1‑143 (MQ=255)                                                                                                                                tttttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTTAGCCTATTTAGACCGTTTCTTCTCCATTTa           >  2:888275/1‑143 (MQ=255)                                                                                                                                       tttATTCAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCACTGCCTTGACTCAGCCTATTCAGACCGTCTCTTCGTCATTCTCGGCGtt    >  2:617125/1‑143 (MQ=11)                                                                                                                                         tattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAt  >  1:235688/1‑143 (MQ=32)                                                                                                                                         tattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATAAGCCTATTTAGACCGTTTCTTCTCCATTTAAGGCATTAt  >  1:48774/1‑143 (MQ=12)                                                                                                                                               |                                                                                                                                         TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  >  NC_000913/2101607‑2101885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.