Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,049,903	IS2 (–) +5 bp	noncoding (5‑9/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4049903 =	0 (0.000)	91 (1.870)	68/282	0.0	100%	noncoding (5/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4049907	0 (0.000)	105 (2.150)	76/282	0.0	100%	noncoding (9/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499378‑4499652                                                                                                                                      |                                                                                                                                              gagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCCGCCGCGGGCTTGTAATGGGTTCAGTGGTAGCAGGGGGCGGGACATATAGGGGCAAATCCACCGTGGGCt                                                                                                                                      >  1:689448/1‑143 (MQ=11)   ggCGTGCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGa                                                                                                                                    <  2:511249/143‑1 (MQ=38)    gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCGGat                                                                                                                                   >  1:798609/1‑143 (MQ=25)      gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATAGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGCGTGAAGTGATACCAGATGTCTGGAAATCTAGGGGCAAATCCACATTGTGCTGatat                                                                                                                                 >  1:360067/1‑143 (MQ=11)          gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGGAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCCAGTCCACCTTGTGCTTACATGaag                                                                                                                             >  1:849540/1‑142 (MQ=11)            gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTCAGAGCAGACGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATAc                                                                                                                           >  1:201649/1‑143 (MQ=255)                tataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGat                                                                                                                       >  1:106032/1‑143 (MQ=255)                  taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                     <  1:428045/143‑1 (MQ=255)                      gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTta                                                                                                                 >  1:679281/1‑143 (MQ=255)                            cATCCGCATAGTGCGCTGGGTTATCGCTCACCACGGGAATATCTGCGGCATCGAGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACATTGTGCTGATATCAAAACGGATATTTTATAAAGa                                                                                                           >  2:286723/1‑143 (MQ=17)                             aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAAAGGGTTAACGGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGaa                                                                                                          >  1:558328/1‑143 (MQ=255)                                      ggggggtGGGGTATCCATCGCTGCGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTGAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  2:575893/137‑1 (MQ=21)                                      ctGCGCTGCGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                                 <  1:321285/142‑1 (MQ=255)                                       tGCGCTGGGTTATCGCTCGCCACAGGAATAACTGGGGCATCGGGCTTGTAATGGGTTAAGTGATGACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTGATACAGGCTACTTGTCAg                                                                                                >  1:610522/1‑143 (MQ=18)                                         cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                                              <  1:741781/143‑1 (MQ=255)                                              ggTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGAAGAGCCACCAGGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                                         >  1:409187/1‑143 (MQ=21)                                                   tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTg                                                                                    >  1:300725/1‑143 (MQ=255)                                                           cACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAg                                                                            >  2:539029/1‑143 (MQ=255)                                                              gggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCt                                                                         >  1:761320/1‑143 (MQ=255)                                                                       tGCGGCAGCGGGCTTGTAATGGGTTAAGTGATCCTCGCTGTCTGGAGATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                                <  2:625088/143‑1 (MQ=38)                                                                       accGGCCGCCGTCTTGTAATGGCTTCAGTGCTATCCGATGTGTGGAACTATAGGGGCACCTAATTCTTGTGCTGATCTGAATACGAATAGTTCAGTCCGTATTATTTTCTGTCCAAGCGCCTGTATTACAGTCTGCCatatat                                                                <  2:824977/141‑1 (MQ=255)                                                                               cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCt                                                        <  1:217419/143‑1 (MQ=255)                                                                               cGGGCTTGCAATGGGTTAAGTGAGAACAGATGGCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATCCGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCt                                                        <  1:226734/143‑1 (MQ=255)                                                                                  gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGTATCCGCACATGTAAGACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtgtg                                                     >  1:411970/1‑143 (MQ=25)                                                                                  gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtgtt                                                     >  1:809674/1‑142 (MQ=255)                                                                                  gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCtgtg                                                     >  1:550595/1‑143 (MQ=255)                                                                                    ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGAt                                                   >  1:68549/1‑143 (MQ=255)                                                                                       tAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAg                                                >  1:167006/1‑143 (MQ=255)                                                                                         aTGGGTTACGTGATAACAGATGTCCGGAAAGATAGTGGCAAATCCACCTTGTGCTGATATGAGTACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGga                                              <  2:14390/143‑1 (MQ=255)                                                                                         aTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGga                                              <  1:831388/143‑1 (MQ=255)                                                                                          tGGGTTAAGTGTTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                                             <  2:281248/143‑1 (MQ=255)                                                                                            ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaaga                                           >  1:616965/1‑143 (MQ=255)                                                                                            ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTATATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATGAGgaaga                                           >  2:104809/1‑143 (MQ=255)                                                                                             gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATGTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaagaa                                          >  1:126205/1‑143 (MQ=255)                                                                                                   tGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTg                                    >  2:286949/1‑143 (MQ=255)                                                                                                      tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCAGATATGATTACCCGTGATCAGGCAGAATAAGTGGCt                                 >  1:304154/1‑143 (MQ=255)                                                                                                         cAGCTGTCCAGAAATTGAGTGGCAAATCCACCTTGTGCTGATATGGATACGGATATTTTATACAGAATCCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg                              <  2:257206/143‑1 (MQ=255)                                                                                                            aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTCCAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGACGAATAAGCGGCGGAggct                           >  2:75588/1‑141 (MQ=255)                                                                                                             tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAGAAGTGGCAGAGGtgt                          >  2:301919/1‑141 (MQ=255)                                                                                                               tCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCa                        >  2:73798/1‑143 (MQ=255)                                                                                                                cTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGGCACAAACAATTATTTGTGGCAAGTAAAAAAAAGTGGCTGAGGTTTCaa                       >  1:791302/1‑143 (MQ=255)                                                                                                                 gggAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                      <  2:405478/142‑1 (MQ=255)                                                                                                                   gAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACt                    >  1:350765/1‑143 (MQ=255)                                                                                                                     aaTATAGGGGCAAAACCACCTTGTGGTGATATGAAGACGTGTATTTTATACAGAAGACTTTTCAGTACAAGCGACGGTATTACAGTCTGCCGTATATGATTAACTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGt                  <  2:551222/143‑1 (MQ=255)                                                                                                                       tataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCt                >  1:105187/1‑143 (MQ=255)                                                                                                                        ataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg               >  2:265472/1‑143 (MQ=255)                                                                                                                        ataGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg               >  1:584407/1‑143 (MQ=255)                                                                                                                         taGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGGATGACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGc              <  1:66201/143‑1 (MQ=255)                                                                                                                                     cACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCct  <  2:201649/143‑1 (MQ=255)                                                                                                                                     cACCTTGTACTGCTATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCct  <  1:599100/143‑1 (MQ=255)                                                                                                                                      |                                                                                                                                              GAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCT  >  NC_000913/4499378‑4499652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.