Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,049,913	IS2 (–) +5 bp	noncoding (15‑19/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4049913 =	0 (0.000)	42 (0.980)	35/284	0.2	100%	noncoding (15/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4049917	0 (0.000)	52 (1.230)	46/280	0.0	100%	noncoding (19/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

TGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4050054‑4049913 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGG  >  NC_000913/4498181‑4498320                                                                                                                                                                                                                                                                                            TGATAAACCTCCCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTAT                                                                                                                                             <  2:368851/143‑1       acaccaCGCATCAGGCATTACGTATCTCTTCTTTCGCCCAGTAAAAAACGCCCCCGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTG                                                                                                                                       <  2:226612/137‑1                 TCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTATGAAAGATAGAAGATCTTATAGAGTGGCCCCCTGca                                                                                                                             >  2:23066/1‑141                      cagTACGGATCTTTTCTTTCTCCTATTAAAAATCGCCCCTGTCATTACTGACTGGGGCGGCTAATATATTAAGCCAAATACGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCC                                                                                                                        <  2:11559/140‑1                      CATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCC                                                                                                                        <  2:199755/143‑1                      CATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCC                                                                                                                        <  2:455550/143‑1                            GGATCTTTTCTTTCGCCCAATAAAAAACGCCCTAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATACTATAGACTGGCCCCCTGAAACTCCCGACAA                                                                                                                  >  2:409407/1‑143                            GGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACAGGCCGCCTGGATCTCCAGACAA                                                                                                                  >  2:122469/1‑143                                          GCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATGCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAA                                                                                                    >  2:653200/1‑143                                              AATAAAAAACGCTCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAA                                                                                                <  1:669625/143‑1                                              AATAAAAAACGCCGCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAA                                                                                                <  2:682479/143‑1                                                    AAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATA                                                                                          <  1:510659/143‑1                                                             AGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATA                                                                                 <  2:180718/143‑1                                                              GTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATAC                                                                                <  2:527573/143‑1                                                                 ATTACTGACTGGGGCGGACAAAATAATCAGCCAAATCCGATTACGTGAAGTAAAAGGACTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAACACATGATAGTCATAATACTAG                                                                             >  2:96295/1‑143                                                                       GACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTA                                                                       <  1:419568/143‑1                                                                       GACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTA                                                                       <  1:84854/143‑1                                                                         CTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGA                                                                     <  2:222611/143‑1                                                                            GGGCGGCTAAAATATACAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCCTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA                                                                  <  1:320159/143‑1                                                                                 GCTAAAAGATTCAGCCGAATCCGATTACGTGAAGGAAAAGGTCTGAAAGAGAGAACATCATATAGACTGGCCCCCTGTATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAT                                                             <  2:128744/143‑1                                                                                   TAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGATTTTAGACTAGTCATTG                                                           >  2:393882/1‑143                                                                                     AAATATTCAGCCAAATCCGAGTACGTGAAGTAAAAGGTCTGAAAGATAGCACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGA                                                         <  1:194453/143‑1                                                                                     AAATATGCAGCCAACGCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGA                                                         <  2:589112/143‑1                                                                                        TATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAA                                                      <  1:490479/143‑1                                                                                         ataCAGCCAAATCCGAGTACGTGAAGAAAAAGGACTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAC                                                     <  1:149554/140‑1                                                                                         ATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACATCCACTATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTAATTGAAAAAC                                                     >  1:46250/1‑143                                                                                             AGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATAATAGTTTTTAGACTAGTCATTGGAGAACAGAT                                                 >  2:373885/1‑143                                                                                                CAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGAT                                              >  1:716456/1‑143                                                                                                CAAAGCCGAGTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTAGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGAT                                              <  2:585954/143‑1                                                                                                 AAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTGGTCATTGGAGAACAGATGATT                                             >  1:270535/1‑143                                                                                                  AATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCAAGACAAGCAATATCACTTAAATACATCATAGTCTAAAGACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTG                                            >  1:773307/1‑143                                                                                                           atGTGAAGTCACAGCTCGGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAG                                   <  2:687633/141‑1                                                                                                           ACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAGGATAGATGTGTTgg                                   >  1:733574/1‑141                                                                                                             tTGACGTAAAAGGTCTGACAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAGATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGG                                 <  2:403027/142‑1                                                                                                                     AAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCCATATCGCTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTGATAGGGCCGGCGAc                         >  2:721797/1‑142                                                                                                                     AAAGGTCTGAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATACGTGATCGCCTTAATACTAGTTTTTAGACTAGTCATTGGAGCACAGATGCTTGCTCGCTCAGGGGCGGAGAg                         >  2:713239/1‑142                                                                                                                             GAAAGATAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTCGAGAACAGATGATTAATGTCTTAGGACCGGAGAAACGCAGAC                 >  2:508764/1‑143                                                                                                                               AAGATAGAACCTCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGG               <  1:231779/143‑1                                                                                                                                   TAGAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGACAATGGAGAACAGAGGATTGATGTCTTAGGGCCGGAGAAGCGCAGAGGGCGTc           >  2:384170/1‑142                                                                                                                                     GAACATCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCAATTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACC         >  2:364905/1‑143                                                                                                                                          TCTTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAACTTATTGTTTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACA    <  1:653200/143‑1                                                                                                                                            TTATAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGACGATTGATGGCTTAGGGCGGGAGAAACGCAGACGGCGGACCACACAGG  >  1:71506/1‑143                                                                                                                                                                                                                                                                                            TGATAAACATAGCGCATCAGGCATTACGGATCTTTTCTTTCGCCCAATAAAAAACGCCCCAGTCATTACTGACTGGGGCGGCTAAAATATTCAGCCAAATCCGATTACGTGAAGTAAAAGGTCTGAAAGATAGAACATCTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4050054‑4049913 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGG  >  NC_000913/4498181‑4498320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.