Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,049,913	IS2 (–) +5 bp	noncoding (15‑19/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4049913 =	0 (0.000)	42 (0.980)	35/284	0.2	100%	noncoding (15/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4049917	0 (0.000)	52 (1.230)	46/280	0.0	100%	noncoding (19/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGG  >  NC_000913/4498045‑4498320                                                                                                                                         |                                                                                                                                            gggATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTg                                                                                                                                       <  1:575297/143‑1 (MQ=255)      tATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGcccc                                                                                                                                  >  2:769779/1‑143 (MQ=255)        tCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAACAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATGTGGTGGTCCCGTGTAGGCTGGCCCCCt                                                                                                                                >  2:556172/1‑143 (MQ=255)        tCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACTTCATAAAAAATCCTGTACTCACAGGGTGATACGGGATAACTGAAGAAATATATAGTTCAGGGAGTTGATGGTCCCTTGTGAACTGGccccgt                                                                                                                                >  2:161796/1‑141 (MQ=255)        tCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATGTGATGGTCCCTTGTAGACTGGCCCCCt                                                                                                                                >  2:592090/1‑143 (MQ=255)               ccGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAAtctc                                                                                                                         <  1:265727/143‑1 (MQ=255)                 ggggTTATTCATTATTTCTATACGTTCGATAAAAAAGTAACCTCATAACAACTCCTGTGCTCAGAGGGTGAGGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCa                                                                                                                       <  2:138621/141‑1 (MQ=255)                 gcgGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAACATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCa                                                                                                                       <  2:49403/143‑1 (MQ=255)                 gcgGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCa                                                                                                                       <  1:552847/143‑1 (MQ=255)                   ggTTAGTCATTAGTTATATACATGCAATAAAAAAGTAACCTCAGAAAAGATCCTGTACTCACAGGGTGATGCGGGATAACTGACGGAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGa                                                                                                                     <  2:182126/143‑1 (MQ=255)                     ttATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACa                                                                                                                   <  1:193813/143‑1 (MQ=255)                      tATTAATTATTTATATACAGTCCATACAAAAGTGACCTGCTAAAAAAGCCTGTACGCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACaa                                                                                                                  <  2:160954/143‑1 (MQ=255)                       aTTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCAAGACAAc                                                                                                                 >  1:580457/1‑143 (MQ=255)                       aTTCATAATTTATCTACATTCAATAAAAAAGTAACCTCCTAAAATATCCTGTACTCACAGGTGGATGCGGTATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAAc                                                                                                                 <  2:678356/143‑1 (MQ=255)                            ttatttatATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTAACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGACCCCTGAATCTCCAGACAACCAata                                                                                                            >  2:165749/1‑143 (MQ=255)                                ttatATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGAAAACAAATATCAc                                                                                                        >  1:222524/1‑143 (MQ=255)                                 gatataCATTCAGTAAGAGAGTAACCTCATAGATGATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACt                                                                                                       <  2:11304/142‑1 (MQ=38)                                      aCATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGG‑CCCCTGAATCTCCAGACAACCAATATCACTTAAATa                                                                                                 >  1:589709/1‑143 (MQ=255)                                        tttCAATAAAAAAGTAACCTCATAAAAAATGCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATaa                                                                                                <  2:737288/142‑1 (MQ=255)                                                aaaaaGTAACCTCATAAAAAATCCTGTACTCACGGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGCTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGt                                                                                        <  2:723294/143‑1 (MQ=255)                                                 aaaaGTAACCTCATAAAGAATCCTGGACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTc                                                                                       <  2:335753/143‑1 (MQ=255)                                                  aaaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTACATAAGTGATAGTCt                                                                                      >  1:262330/1‑143 (MQ=255)                                                              tAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGttt                                                                          >  1:357101/1‑143 (MQ=255)                                                               aaaaaaTCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCGATATCACTTAAATAGATGATAGTCTTAATAcaggagct                                                                         >  2:744116/1‑136 (MQ=37)                                                                 aaaaTCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTa                                                                       <  2:176716/143‑1 (MQ=255)                                                                  aaaTCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATGTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGGCTTAATACCAGTCTTTAg                                                                      >  2:723796/1‑143 (MQ=255)                                                                      ccTGTACTCACAGTGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTGTTTAGACTa                                                                  <  2:23486/143‑1 (MQ=255)                                                                          tccTCACAGGGTGATGCGAGATAACTGACGTAAGGAGCGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCa                                                              <  2:350937/141‑1 (MQ=255)                                                                           aCTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAt                                                             <  1:445529/143‑1 (MQ=255)                                                                             tCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTg                                                           <  2:237016/143‑1 (MQ=255)                                                                               acaGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGga                                                         <  1:19609/143‑1 (MQ=255)                                                                               acaGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGATCCCTTGTAGACTGGCCCCCTGAATCTCTAGACGACCAATATCCCATAGATAAGTGATAGTCTTAATACTAGCTTTTAGACTAGTCATTGga                                                         >  2:586241/1‑143 (MQ=25)                                                                                   ggTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAc                                                     <  2:504521/143‑1 (MQ=255)                                                                                         gCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatga                                               <  2:552808/143‑1 (MQ=255)                                                                                         gCGGGATAACTGACGCAATGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGGTAGTCTTAAGACTCGTTTTTAGACTAGTCATTGGAGAACAgatga                                               <  2:778515/143‑1 (MQ=18)                                                                                             gATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattga                                           <  2:434946/143‑1 (MQ=255)                                                                                             gATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattga                                           <  2:700460/143‑1 (MQ=255)                                                                                              agaaCTGACGTCAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCTTTGGATAACAGAtgattgat                                          <  2:97250/141‑1 (MQ=255)                                                                                              aTAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattgat                                          <  1:370967/143‑1 (MQ=255)                                                                                               tAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGGCATTGGAGAACAGAAGATTGATg                                         >  2:282537/1‑143 (MQ=255)                                                                                                   tGACGTAAGGAGTGCTGCAGTGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCtt                                     <  2:146199/143‑1 (MQ=255)                                                                                                        tAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAACCTCCAGACAACCAATATCACTCAAACAAGGGAGAGTCTTAATACTAGTATTTAGACTAGTCATTGTAGAACAGATGATCGATGTCCTAGGGc                                >  2:192627/1‑143 (MQ=18)                                                                                                           ggAGTGCTGCGGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGGCATAGGAGAACAGATGAATGATGTCCTAGGGCCgg                             >  2:560418/1‑143 (MQ=17)                                                                                                            gAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGCAGACCAGATGATTGATGTCTTAGAGCGGga                            >  2:419205/1‑143 (MQ=255)                                                                                                                gctccAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaaa                        <  2:76687/143‑1 (MQ=255)                                                                                                                      gggATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGa                  <  1:769779/143‑1 (MQ=255)                                                                                                                          tttGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGc              <  1:513146/143‑1 (MQ=255)                                                                                                                            tGATGGTCCCTTTTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGt            <  1:445396/143‑1 (MQ=25)                                                                                                                               tGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTccc         >  1:591722/1‑143 (MQ=38)                                                                                                                                      tcgTAGACTTGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAgg  <  2:81777/141‑1 (MQ=11)                                                                                                                                         |                                                                                                                                            GGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGG  >  NC_000913/4498045‑4498320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.