Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	63 (1.470)	42/284	0.1	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	51 (1.190)	35/284	0.2	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289975‑2290253                                                                                                                                           |                                                                                                                                             tcaaTCTCTTTTCTGGTCTGACGGCGCTTAATGACATTGAAACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATaa                                                                                                                                          <  2:757175/141‑1 (MQ=11)     tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGcc                                                                                                                                      >  2:426639/1‑142 (MQ=21)       gctTGTCTGGTCTGACGGCGCTTACTGCTGAATTGACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                    <  2:78106/142‑1 (MQ=255)        ctTTTCTGGTCTGTCGGCGCTTACTCCTTGATGCACTGTCGGCGAAGGTAAGTTGATGTCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                   <  2:157266/143‑1 (MQ=14)          tttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGc                                                                                                                                 >  1:421295/1‑143 (MQ=40)                  cTGAGGGCGCGGACCGCTGGATGCCCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCGTCCATAACGCTGTAGCCACCAGaa                                                                                                                         <  2:298116/143‑1 (MQ=14)                   tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCCCCAGAAc                                                                                                                        >  2:537963/1‑143 (MQ=255)                           cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTg                                                                                                                >  1:276864/1‑143 (MQ=255)                            ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAAGATGTGGCCACCAGAACAGACATTGc                                                                                                               >  2:169893/1‑143 (MQ=255)                                tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAACCATTATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                           <  1:699409/143‑1 (MQ=255)                                tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATACCGCTGTAGCCACCACAACAGAAATTACCGaa                                                                                                           >  2:238734/1‑143 (MQ=14)                                  ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGCAGCCACCAGAACAGATATCGCGGAACg                                                                                                         >  2:137833/1‑143 (MQ=255)                                    gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAc                                                                                                       <  1:103679/143‑1 (MQ=255)                                     aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACCGATCTTGCGGAACGCCa                                                                                                      >  2:328145/1‑143 (MQ=255)                                     aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACa                                                                                                      >  1:528362/1‑143 (MQ=255)                                     aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATATCATGTCAGGGACTTGTTCGCACATTACATAACGCTGTAGACACCAGAACAGATATTGCGGCACGACa                                                                                                      >  2:443902/1‑143 (MQ=18)                                       ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                    <  1:166495/143‑1 (MQ=255)                                          gctttCGGCGAATGGAAGTTGATGACTCACGCCGAGCCCTGCCCTAGGGCACCAGATCACAAACATGATCACACATCAGGGACTTGTGAGCAGCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAagag                                                                                                 <  2:510904/139‑1 (MQ=255)                                                            ttGATGACTCATGATGATCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                               <  2:123745/143‑1 (MQ=255)                                                            ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                               >  1:129387/1‑143 (MQ=255)                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGCCAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                       >  1:774614/1‑143 (MQ=255)                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                       >  1:395255/1‑143 (MQ=255)                                                                    tCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCTAATATCAGGGAGTTGTTCGCACATTAGATAATGATTTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAACAGAGTGATGCATTggg                                                                       >  2:720036/1‑141 (MQ=21)                                                                            aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCTCACTAACGCTGTAGCCACCAGAACAGATATTGCGGACCGACAAAGAGAAACAGAAACAGACTCAGGCATGGACCGTTCATc                                                               >  2:128551/1‑143 (MQ=21)                                                                               ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTa                                                            >  1:410353/1‑143 (MQ=255)                                                                                   ttCTATGGCTCCAGATGACAAACATGATCTCATATCCGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaa                                                        <  2:162035/143‑1 (MQ=255)                                                                                       aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTTTTCGCACCTTCCATAACACTATAGCCATCAGAACAAATATTGCGGAACGACAAAGAAAAACAGAACCAGATTGATGCATTGAACTTTTATACTATGAaatta                                                    >  2:706224/1‑143 (MQ=25)                                                                                       aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACTAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATGCTATGGattta                                                    >  1:37180/1‑143 (MQ=255)                                                                                           cTCCAGATGACAAACCTGATCTCATATCATGGACTGGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCACATTGTTGCATTGAGCTTTCATCCTATGAAATTAATTg                                                <  1:404302/143‑1 (MQ=255)                                                                                           cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTg                                                <  2:324655/143‑1 (MQ=255)                                                                                           cTCCAGATGACAAACATGATCTCAAATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTg                                                <  1:424262/143‑1 (MQ=255)                                                                                               aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAACCAGAACAACACTAGACAAGTGAGCTTTCATCCCATGAAAATAATGGCTGt                                            >  2:549568/1‑143 (MQ=25)                                                                                                    aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCACGACAAAGAGAAACAGAACCAGATTGATGCATCGAGCGTTAATCCTATGAAATTAATTGCTGTTaaac                                       >  2:775568/1‑142 (MQ=255)                                                                                                         cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                  >  1:602928/1‑143 (MQ=255)                                                                                                          aTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTg                                 >  2:433631/1‑143 (MQ=255)                                                                                                          aTGATCGCATATCAGGGACTTGTTCGGACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTg                                 <  1:761429/143‑1 (MQ=255)                                                                                                               ctcATAGCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTAc                            >  2:521784/1‑143 (MQ=255)                                                                                                                tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACa                           >  2:726906/1‑143 (MQ=255)                                                                                                                     tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTAGGTACAGaaaa                      >  1:13451/1‑143 (MQ=255)                                                                                                                        gggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAGTTGCTGTTAAAAGCATTGGGTACAGAAAATAc                   <  2:410353/143‑1 (MQ=255)                                                                                                                        gggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTCCAGAAAATAc                   >  1:615548/1‑143 (MQ=255)                                                                                                                        gggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc                   <  2:528362/143‑1 (MQ=255)                                                                                                                        gggACTTGTTCGAACCTTCCATAACGCTGAAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc                   <  2:165149/143‑1 (MQ=255)                                                                                                                                ttCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCt           <  2:395255/143‑1 (MQ=255)                                                                                                                                 tCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTc          <  1:281093/143‑1 (MQ=255)                                                                                                                                   gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCa        >  1:572148/1‑143 (MQ=255)                                                                                                                                    cACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAt       >  1:190657/1‑143 (MQ=255)                                                                                                                                         tCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGCAACAGCACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTATTTGCTGTTACAAGCATTGGGTACAGAGAATACCCATAACTCCATACCCg  >  2:551333/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                             AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCG  >  NC_000913/2289975‑2290253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.