Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	1 (0.020)	54 (1.020)	45/284	0.2	98.2%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	1 (0.020)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001503‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001650‑4001781                                                                                                                                                                                                                                                                                    AGTGTGGATTGAGCGTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATC                                                                                                                                     <  1:620684/143‑1  GTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCT                                                                                                                                    >  2:772645/1‑143    ATGGAGTGGTCTTGGCGAACGGGACGACGACGAGCGACTGCGCGTACAATCTGAACTGGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCC                                                                                                                                  <  1:447447/143‑1      ggtcTGATCGTGTCGCATCGGACGGCGACGCGCGACTGCGCGTACAATCGGAACTTGGCCGGAGCCTGGCAACCCGCCCGGCACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTT                                                                                                                                <  2:129849/139‑1            ATCTTGTCGAACCGGACGACGACGAGCGGCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTT                                                                                                                          >  1:408648/1‑143               TTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGCAGCATCGGCACGTTTCTTTGAAGACGACGCCGGCCTGCATCCCCTTCTTCTTCTT                                                                                                                       >  1:792063/1‑143                            CGACGACGGGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAA                                                                                                          <  2:400402/143‑1                                GACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTCTGAAGACGACGACGGCCTGCATCTCCTTCTTCTCCTTTGAAGATGCGGAAGAcc                                                                                                      >  2:153976/1‑141                                     GCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCC                                                                                                 <  2:174188/143‑1                                        ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGTATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                              <  1:72341/143‑1                                         CTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCAGCACGGAACTGGAAGACATCGAAACATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGCTGCGGAAGATCTCTCCGGTc                                                                                             >  1:205523/1‑142                                          ttCGCGTACAATCTGAACTCCGCCAGCGCCTGGCAACCCGCCCGGAACTGGAAGCCCTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAA                                                                                            <  1:108681/141‑1                                            CGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACT                                                                                          >  2:146585/1‑143                                                    ATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                                  <  2:743883/143‑1                                                    ATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                                  <  2:937897/143‑1                                                       TGAAGTGGGCCAGAGCCTGGCAACCCGCCCGGCACTGGAGGTCGTGGTCGCAGCGGCACGTTTCTTTGAACTCGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCA                                                                               <  1:904600/143‑1                                                          ACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGAGACCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGGCATTT                                                                            <  2:936746/143‑1                                                          ACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGCCGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTT                                                                            <  1:47634/143‑1                                                           CTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTA                                                                           <  1:729728/143‑1                                                                CCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATC                                                                      >  1:863203/1‑143                                                                     GCCTGGCAGCTCGCCCGGCACTGGAAGACATCGAAGCATCGTCACGTTTCTTTGAAGACGACGACGGCCTGCATGTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACCCCACTGTGGCATTTACCATCCGTGA                                                                 <  2:242026/143‑1                                                                      CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCAGCGGCACGGTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT                                                                <  2:401963/143‑1                                                                       CTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCCTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTATGGCATTTACCATCCGTGAcg                                                               >  1:760183/1‑141                                                                         GGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGT                                                             >  2:791833/1‑143                                                                          GCAACCCTCCCGGAACTGGAAGACATCGAAGCGTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTC                                                            <  2:301731/143‑1                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATTACAATGTGATCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       >  1:944199/1‑143                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       <  1:208378/143‑1                                                                               CCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTG                                                       >  2:652391/1‑143                                                                                CGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGT                                                      <  2:841058/143‑1                                                                                 GCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTT                                                     <  2:449305/143‑1                                                                                  CCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTc                                                    >  2:558803/1‑142                                                                                     GGAACGTGAAGACATCGAAGCATCGGCGCGTTTCTTTGAAGACGGCGACGGGCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCCCGCCGGTAACTCCACTGTGGCATTTACCATCCGTGGTGGTCGTCTTTTTACT                                                 <  2:388988/143‑1                                                                                        ACTGGAAGACATCGAAGCATCGGCACGATTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTG                                              >  2:527295/1‑143                                                                                         CTGGAAGACAGCGAAAAACCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGC                                             <  1:253003/143‑1                                                                                            GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTG                                          >  1:453153/1‑143                                                                                              AGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAG                                        >  1:356596/1‑143                                                                                               GACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGC                                       <  1:203611/143‑1                                                                                                  ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGTCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                                    <  1:839628/143‑1                                                                                                   TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTCACTCCACTGTGGCATTTACCATCCGTGATGGTCGCCTGTTTACTCTGCGTGAGCGTGA                                   >  2:696004/1‑143                                                                                                    CGACGCACCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTTTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  1:229819/143‑1                                                                                                    CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  1:772645/143‑1                                                                                                    CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                  <  1:746941/143‑1                                                                                                     GAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAAC                                 <  1:676378/143‑1                                                                                                              GCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGGCGTCTGTTTACTCTGCGTGAGCGTGAACTACCCGCTT                        >  2:738332/1‑143                                                                                                                  GTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGGGCGTGGACTGCCCGCTTTTCG                    >  1:938486/1‑143                                                                                                                  GTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAGCACGCCGGTAACTCCCCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCG                    >  1:803242/1‑143                                                                                                                    TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                  >  1:47221/1‑143                                                                                                                    TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                  >  1:49394/1‑143                                                                                                                     TCTTTGAAGGCGACGACGGCCTGCATCTCCTTCTGCTTCTTTGATGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT                 <  2:234260/143‑1                                                                                                                          GAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATC            >  1:786253/1‑143                                                                                                                                GACGACTGCTTGCATCGCCTTCTTCTTCGGTGGGGATGCGGAAGATCAGGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCGGCGTGAGCGTGAACTGTCCGCTTTTCGTCTGTATCGTATGC      <  2:377453/143‑1                                                                                                                                GACGACGGCCTGCGACTCCTGCTTCTCCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGC      <  1:146585/143‑1                                                                                                                                GACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACTATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGC      <  1:746965/143‑1                                                                                                                                    ACGGCCCGCAGCTCCCTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGCCATTCAACCTCCGTTAGGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  <  1:854908/143‑1                                                                                                                                                                                                                                                                                    AGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001503‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC  >  NC_000913/4001650‑4001781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.