Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,937,136	IS2 (–) +5 bp	coding (485‑489/870 nt)	yebK →	putative DNA‑binding transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1651548	NA (NA)	51 (0.960)	42/284	0.2	98.0%	noncoding (706/706 nt)	IS2	repeat region
	?	NC_000913	= 1937140	1 (0.020)					coding (489/870 nt)	yebK	putative DNA‑binding transcriptional regulator
*	?	NC_000913	1937136 =	1 (0.020)	59 (1.110)	44/284	0.2	98.3%	coding (485/870 nt)	yebK	putative DNA‑binding transcriptional regulator
	?	NC_000913	= 2070271	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATT  >  NC_000913/1651414‑1651690                                                                                                                                       |                                                                                                                                               agagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGaa                                                                                                                                        <  1:805653/143‑1 (MQ=35) agagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGAGGCAAATCcacatcgcgc                                                                                                                                        >  2:558840/1‑137 (MQ=2)       gTTCGAGCATTATAACGAGTGGCATCCGCATCGTGCGCTGGGTTCTCGGTCGCCATGGGACTATATGCGGAAGCGGGATTGTAGTGGGTTACGTGATAACAGATGTCTGGAAATATAGGGGCAAATCTCCAGGTGAACTACGc                                                                                                                                  >  1:162863/1‑143 (MQ=11)              cATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCAAGGGACTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCGCCTCtg                                                                                                                           >  2:394120/1‑143 (MQ=255)                 tataACGAATGGCATCCCCATCGTTCGCTTTGTTATCGCTTAATACCGTTGTTGCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCtgttt                                                                                                                        <  1:214915/143‑1 (MQ=17)                  ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTACTGGGCTAAGTGATAGCAGATGTCCGGAAATTTAGGGGCAAATCCACAGGTGAACTACGCTCCTCtgtttg                                                                                                                       <  1:873176/143‑1 (MQ=17)                      tgcaTGGCATCCGCATAGTGCGCTAGGTCAGCGCACGCCACGGGAATATCCGCGGCAGCGGGCTTGGAATGGGTTAAGTGATAACAGATGTCTCGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                                                   <  1:247607/140‑1 (MQ=17)                      cGAATGTGATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                                                   <  1:759119/143‑1 (MQ=255)                       gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACg                                                                                                                  >  1:582246/1‑143 (MQ=255)                             cATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATACACGGTGACCGCAAACATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaa                                                                                                            >  1:696479/1‑143 (MQ=18)                              aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaaa                                                                                                           <  1:869174/143‑1 (MQ=255)                              aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaaa                                                                                                           >  1:791836/1‑143 (MQ=255)                                ctgCATAGTGCGCTGGGTTATCGCTCGCCACGGGAAACGCATTGGTAGCGTGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaaaaa                                                                                                         <  2:421312/141‑1 (MQ=18)                                   cATAGAGCCCTGCGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTg                                                                                                      <  2:229080/143‑1 (MQ=255)                                      aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGgtg                                                                                                   >  1:463616/1‑143 (MQ=255)                                      aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGgtg                                                                                                   >  1:729286/1‑143 (MQ=255)                                        tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCGCTGTTTGTTACGCAATAAAAACTGGTGtt                                                                                                 >  1:511463/1‑143 (MQ=255)                                          cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGtttt                                                                                               <  1:681349/143‑1 (MQ=255)                                           gcTGGGTTAGCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTc                                                                                              <  1:390870/143‑1 (MQ=255)                                             ggggtgttcgcgcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGGGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                                            <  2:302874/136‑1 (MQ=255)                                                     cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCGTGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTTGTTGCTACGCAATAAAAACTGGTTTTTTCCACTTTGaaa                                                                                    >  1:204598/1‑143 (MQ=18)                                                      gctcgcCACGGGACTGTCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaaa                                                                                   <  1:274383/143‑1 (MQ=255)                                                       ctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaaaa                                                                                  <  1:72113/143‑1 (MQ=255)                                                        tcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAAc                                                                                 >  1:336214/1‑143 (MQ=255)                                                             aagggAATACCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGttt                                                                            <  1:748547/141‑1 (MQ=255)                                                             aCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTAGAAATATAGGGGCAAATCCACAGGTGAACTACGATCCTCTGTTTGTTACGCAATAAAAACTGGTGATTTCGACCATGCAAAACAGttt                                                                            >  2:372786/1‑143 (MQ=255)                                                                      tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCa                                                                   >  1:614186/1‑143 (MQ=255)                                                                      tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCATCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCctcctcc                                                                   >  1:543316/1‑142 (MQ=255)                                                                        tGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAATCACACACCCCCCAACATATCCTAATGTAAACTGGTGTTTTCGACTATGCACAACAGTTTCCATCCTCACg                                                                 >  2:424214/1‑143 (MQ=9)                                                                              aGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCt                                                           >  1:101117/1‑143 (MQ=255)                                                                                  ggCTTGTAAGGGGTTAAGTGAGAACATGTGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCGCCTCTGGTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaa                                                       <  2:391129/143‑1 (MQ=38)                                                                                   gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaa                                                      >  2:845822/1‑143 (MQ=255)                                                                                     ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaaaa                                                    >  2:254551/1‑143 (MQ=255)                                                                                     ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaaaa                                                    >  2:626100/1‑143 (MQ=255)                                                                                     ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaaaa                                                    >  1:192284/1‑143 (MQ=255)                                                                                       gtgaTGGGTTAAGCGATAACAGATGTCTGGGAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAAtt                                                  <  2:511463/140‑1 (MQ=255)                                                                                       gTAATGGGTTAAGTGCTAACAGATGTCCGGAAATATAGGGGCAAATCCACAGCTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAAtt                                                  <  2:689269/143‑1 (MQ=255)                                                                                        tAATGGGTTAAGTGATAACAAATGTCTGCAAATATAGAGGCAAATATACATGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTTGTGTTTTCGACGATGCAAATCAGTTTCCCTCCTCACGCAATCTGGAAAAAttt                                                 <  1:851787/143‑1 (MQ=38)                                                                                         aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTg                                                <  2:791836/143‑1 (MQ=255)                                                                                          aTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACGACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTGCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTgg                                               <  2:305180/143‑1 (MQ=255)                                                                                                  aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGccc                                       >  1:808720/1‑143 (MQ=255)                                                                                                     gtttaCAGTTGTCTGGAAATTTATGGGCAAATCCACAGGTTAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGAc                                    <  2:552578/139‑1 (MQ=255)                                                                                                       tAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGAGaa                                  >  2:939844/1‑143 (MQ=255)                                                                                                          cAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCCCCTCACGCAATCTGGAAACATTTGGTGAGGCCCGACAAGAt                               >  2:806585/1‑143 (MQ=255)                                                                                                              tGTCTGGATATACGGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTaa                           <  2:707061/143‑1 (MQ=255)                                                                                                              tGTCTGGAAATACAGGGGCAAATCCACAGGTGAACGACGCTCCTCTGTGTGTTACGCAATAAAAACGGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTaa                           <  2:723926/143‑1 (MQ=255)                                                                                                                tCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAcc                         >  1:749849/1‑143 (MQ=255)                                                                                                                  tGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCAt                       >  2:398028/1‑143 (MQ=255)                                                                                                                  tGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAAATGGTGTGGTCGACTATGCAAAACAGCTTCCCTCCTCACGCAATCTGGAAAACTTTGGTGAGGCCAGACAAGGTCTAACCAt                       >  2:464386/1‑143 (MQ=255)                                                                                                                             gggCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTTGTGAGGCCCGACAAGATTTAACCATAAAAGAACTgg            <  1:682272/143‑1 (MQ=255)                                                                                                                              ggCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGc           <  1:163029/143‑1 (MQ=255)                                                                                                                               gCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGCAAAATTTGGTTAGGCCCGACAAGATTTAACCAGAAAAGAACTggat          >  1:127584/1‑141 (MQ=255)                                                                                                                                      cacaGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAAt   <  2:729286/143‑1 (MQ=255)                                                                                                                                      cacaGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCACCTCCCGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAAt   <  2:463616/143‑1 (MQ=255)                                                                                                                                       acaGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAACCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAAtt  >  2:451362/1‑143 (MQ=255)                                                                                                                                       |                                                                                                                                               AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCTGAAAAATT  >  NC_000913/1651414‑1651690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.