Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,913 IS2 (–) +5 bp noncoding (15‑19/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4049913 =1 (0.020)71 (1.340) 50/284 0.1 98.6% noncoding (15/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 40499170 (0.000)71 (1.360) 58/280 0.0 100% noncoding (19/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

TTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTC  >  NC_000913/4499373‑4499650
                                                                                                                                          |                                                                                                                                           
ttctagagGCGTTCGTGCTTTCTGACTTGTGGCATCCGTGTAGTGCGCTGGGTTATCGCTCGCCACGGGCATATCTTCTGCAGCGGGCGTGTAATGGGTTAAGTGATAACTGATGTCTGGTTATATAGGTGCAAATCCACCtt                                                                                                                                         <  2:732073/139‑1 (MQ=2)
ttGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCGGGTAATGGGTGAAGTGATAGCAGATGGCTGGAGATATAGGGGCGAATCCGCCtt                                                                                                                                         >  2:729470/1‑143 (MQ=11)
  gCAGCGGCGTTCGAGCTTTGGAACGAAGGGCGTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATGTCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgt                                                                                                                                       <  2:129489/143‑1 (MQ=11)
    agagGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGTGCTTGTAATGGGTTAAGTGATAAAAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGc                                                                                                                                     >  1:384574/1‑143 (MQ=25)
                  aatataACGAATGGCATCCGCATAGTGCGCTGGGTTTTCGCTCTCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                                       <  1:80083/141‑1 (MQ=255)
                     ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATCTGAAGACGGatc                                                                                                                    >  1:592279/1‑142 (MQ=255)
                     ataACGAATGGCATCCGCATAGTGCGATGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCATGTAAGGGGTCAAGTGATAACAGATGACTGGAAACATAGGCGAAACTCCACCGTGTGCTGATATGAACACGGcta                                                                                                                    >  2:189960/1‑143 (MQ=14)
                      taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGCATACGGatat                                                                                                                   >  2:57571/1‑143 (MQ=255)
                      taACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGatat                                                                                                                   >  1:917645/1‑143 (MQ=255)
                       aaCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAtt                                                                                                                  >  1:653993/1‑143 (MQ=255)
                        acggagggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTCGTCATGGGTTACTTGATAACAGACGTCTCGAAATATCGGGGCACATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                                 <  2:409201/137‑1 (MQ=17)
                          gagtGGCATGCGCATGGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGGGGGCGTGTAATGGGTTAAGTGATAACGGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTta                                                                                                               <  2:345183/140‑1 (MQ=17)
                           aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGCTGTCTGGAACTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGCATTTTTtat                                                                                                              >  1:191002/1‑143 (MQ=255)
                            aTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtata                                                                                                             <  1:953705/143‑1 (MQ=255)
                               gCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAg                                                                                                          >  2:430923/1‑143 (MQ=255)
                                 aTCCGCCTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATGTCATGCAGaa                                                                                                        >  2:541593/1‑143 (MQ=255)
                                     gCAGAGTGCTCTGGGTTATCGCTCGCCATGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACt                                                                                                    <  1:91178/143‑1 (MQ=255)
                                      cATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                                                   <  2:183648/143‑1 (MQ=255)
                                        tAGTGCGCTTGGGTATCGCTCGCCACGGGAATATCTGCGGCAGAGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAACATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                                 <  1:837034/143‑1 (MQ=255)
                                        tAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                                 <  2:270379/143‑1 (MQ=255)
                                        tAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTATAATGGGTTACGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                                 <  2:828822/143‑1 (MQ=255)
                                           tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAg                                                                                              >  2:733265/1‑143 (MQ=255)
                                           tGCGCTGGTGATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGt                                                                                             <  2:555408/143‑1 (MQ=255)
                                            gcggTGGGTTATGGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGt                                                                                             <  2:258042/143‑1 (MQ=255)
                                                  ggTTATCGCTCGGCCGGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                                       <  2:597057/143‑1 (MQ=255)
                                                      aTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACt                                                                                   >  1:234679/1‑143 (MQ=255)
                                                        cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCCGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGt                                                                                 >  1:205331/1‑143 (MQ=255)
                                                              ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACa                                                                           <  2:35908/143‑1 (MQ=255)
                                                                     aaTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGcc                                                                    <  1:322907/143‑1 (MQ=255)
                                                                          gtGCGGCAGCGGACTTTTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAACTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatc                                                               <  2:113460/142‑2 (MQ=255)
                                                                           tGCGGCAGCGGGCGTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                              <  2:461271/143‑1 (MQ=255)
                                                                            gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATg                                                             >  1:758940/1‑143 (MQ=255)
                                                                                    gggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATCTGATTACCtg                                                     >  1:268899/1‑143 (MQ=255)
                                                                                              tGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTGGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                                           <  1:819515/143‑1 (MQ=255)
                                                                                                 gtgaaGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGCGATAAGgaagaa                                        >  2:345520/4‑143 (MQ=255)
                                                                                                  ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCGTATATGATTACCTGTGATCAGGAAGAAt                                       >  1:836914/1‑143 (MQ=255)
                                                                                                  ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                                       >  1:407854/1‑143 (MQ=255)
                                                                                                  ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                                       >  1:211407/1‑143 (MQ=255)
                                                                                                    aactGATATCAGATGTCTGGAAATATAGGGGCAAATCCATCTTGTGCTGATATGAATACGGATATTTTATAGAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATTTATGATTACCTGTGATCAGGAAGAATaa                                     <  2:704904/140‑1 (MQ=255)
                                                                                                     aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACGTGTGATCAGGAAGAGTAAg                                    >  1:9481/1‑143 (MQ=255)
                                                                                                     aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAg                                    >  1:332546/1‑143 (MQ=255)
                                                                                                      gaaaTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGAGATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGt                                   >  1:548148/4‑143 (MQ=255)
                                                                                                      gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAgc                                   >  2:249053/1‑142 (MQ=255)
                                                                                                      gTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGt                                   <  1:751579/143‑1 (MQ=255)
                                                                                                         aTAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATCTCTGATTGCTTGTGATCAGGAAGAATAAGTGGc                                >  1:174495/1‑143 (MQ=255)
                                                                                                           aaCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTg                              >  2:2275/1‑143 (MQ=255)
                                                                                                            aCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGa                             >  2:466922/1‑143 (MQ=255)
                                                                                                             cAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTGCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAg                            <  1:663542/143‑1 (MQ=255)
                                                                                                                aTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGtt                         >  1:841775/1‑143 (MQ=255)
                                                                                                                   tCTGGAAATATAGGGGCAAATCAGCCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCTGTCTATAATTACCTTTGATCAGGAAGAATAAGTGGCTGAGGTTTCa                      <  2:427940/143‑1 (MQ=255)
                                                                                                                     tGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                    <  1:430923/143‑1 (MQ=255)
                                                                                                                      ggAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAAc                   <  1:42382/143‑1 (MQ=255)
                                                                                                                               ggggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCg          >  1:332722/1‑143 (MQ=255)
                                                                                                                                gggCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACGGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg         >  1:925199/1‑143 (MQ=255)
                                                                                                                                  ggtaaTCCACCTTGTTCTGTTAGGAATACGGATATTTTATACAGAATACTTTTCAGGACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGta       <  1:666711/140‑1 (MQ=255)
                                                                                                                                  gCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTGCAAACTGTCTGCCGGta       >  1:156968/1‑143 (MQ=255)
                                                                                                                                       tCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGGATATTc  >  2:270975/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTC  >  NC_000913/4499373‑4499650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.