Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,992,705	IS5 (+) +4 bp	coding (1553‑1556/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	53 (1.360)	40/282	0.1	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3992705 =	0 (0.000)					coding (1553/2547 nt)	cyaA	adenylate cyclase
*	?	NC_000913	= 2290113	NA (NA)	55 (1.410)	32/282	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3992708	0 (0.000)					coding (1556/2547 nt)	cyaA	adenylate cyclase

CTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2288787‑2289057                                                                                                                                     |                                                                                                                                           cTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGa                                                                                                                                  <  2:535189/143‑1 (MQ=255) cTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGa                                                                                                                                  <  1:578585/143‑1 (MQ=255)          gCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTCTGGGTGCATATGAGGGAGGTGCGAATAAGCggg                                                                                                                         >  1:674101/1‑143 (MQ=255)           ccAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCgggg                                                                                                                        <  2:51912/143‑1 (MQ=255)                    attTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCGATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAttcttc                                                                                                               >  1:661795/1‑143 (MQ=255)                      tttCGTATTATTGGCGGTCCCATAAACGAGCTTGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGGATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                             >  1:103235/1‑143 (MQ=255)                         cGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                          <  1:636885/143‑1 (MQ=255)                           tattatTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACGCAACGGTTGATGCCACTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAGATTCTTCTCGGCTg                                                                                                        >  1:584439/1‑143 (MQ=255)                               attGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTc                                                                                                    >  1:304305/1‑143 (MQ=255)                                ttGGCAGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                   <  2:333987/143‑1 (MQ=255)                                     gggcccATATGCCAGCTGGATAGCGTTTTCCAGGACAACACCAGCGACTGTCTCACCATACTCAACAGGTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                              <  2:25846/140‑1 (MQ=37)                                      gTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                             >  2:28620/1‑143 (MQ=255)                                      gTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                             >  1:704511/1‑143 (MQ=255)                                           aTAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcgtttcatt                                                                                        >  1:25953/1‑143 (MQ=255)                                                          ctcttctACCAGGACAACACCATCCACTGTCTCGCCATACTCAACGGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                         <  2:571705/142‑1 (MQ=25)                                                             tttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCATTTCTTCATGTTTGAGCCGa                                                                      >  2:247115/1‑143 (MQ=255)                                                              ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                     >  1:579943/1‑143 (MQ=255)                                                                   agtgtcaacgCCATCAACTGTCTGACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGTATATGCGGGTAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                <  2:421194/138‑1 (MQ=21)                                                                           cacCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAACATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTCCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCgaa                                                        >  2:64011/1‑141 (MQ=255)                                                                                tCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCTAATAAGCGGGTAAACTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGc                                                   <  1:117337/143‑1 (MQ=255)                                                                                 cAACTGTCTCATCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                  <  2:31143/143‑1 (MQ=255)                                                                                   aCTGTCTCACCATACTCCTCATTTTGTGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                                <  1:481080/143‑1 (MQ=17)                                                                                   aCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                                <  2:272844/143‑1 (MQ=255)                                                                                     tGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGACTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGATAGAACCGATTTTTTCTCACGTAAAAGCCTTGa                                              >  1:278964/1‑143 (MQ=21)                                                                                         tcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                          <  1:339644/143‑1 (MQ=255)                                                                                         gtatcgtACTCCACAGTAGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                          <  2:643865/137‑1 (MQ=255)                                                                                          cACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                         >  2:652455/1‑143 (MQ=255)                                                                                                  tCAACAGTTGATGCCAATGCTATGGGTGACTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTGCATTTGTTGATGTTTGAGCCCATTTTTTCTCCCGTACATGCCTTGAATCGGCGTCTTTa                                 >  2:659269/1‑143 (MQ=17)                                                                                                  tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTGAGAGCGGAGGTTTTCTCCCGTGAGTGACTTGAAACAGccggttga                                 >  1:42292/1‑137 (MQ=17)                                                                                                    aaCTGTTGATGCCAATGCTAGGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                               <  1:415730/143‑1 (MQ=255)                                                                                                    aaCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAGTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATGTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGa                               >  2:272053/1‑143 (MQ=255)                                                                                                        gTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                           >  1:111931/1‑143 (MQ=255)                                                                                                        gTTGATGCCAATGCGATGGGTGAATATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                           <  2:295683/143‑1 (MQ=255)                                                                                                         ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                          >  1:579940/1‑143 (MQ=255)                                                                                                              gCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTCATGTTTGAAACAATGGTGTCTTCCGTAAATGCCTTGAATCAGTCTATTTAGACGGGttcttc                     >  1:558955/1‑143 (MQ=14)                                                                                                                cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGGTTCTTCgt                   >  1:183232/1‑142 (MQ=255)                                                                                                                         gggTGAATATGCGGATGGTGCGAATAAGCGGTGAAATTCTTCTCCGCTGACTCAGTCATTTCATTTCTTCATGCTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg          <  2:193919/143‑1 (MQ=11)                                                                                                                           gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg        >  1:81044/1‑143 (MQ=35)                                                                                                                                tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGTATCAGCCTATTTAGACCGTTTCTTCGACATTTAAGACGTTATc   >  1:644641/1‑143 (MQ=9)                                                                                                                                 ataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc  <  2:674101/143‑1 (MQ=11)                                                                                                                                     |                                                                                                                                           CTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC  >  NC_000913/2288787‑2289057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.