Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,992,705	IS5 (+) +4 bp	coding (1553‑1556/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	53 (1.360)	40/282	0.1	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3992705 =	0 (0.000)					coding (1553/2547 nt)	cyaA	adenylate cyclase
*	?	NC_000913	= 2290113	NA (NA)	55 (1.410)	32/282	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3992708	0 (0.000)					coding (1556/2547 nt)	cyaA	adenylate cyclase

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAT  >  NC_000913/2289972‑2290248                                                                                                                                              |                                                                                                                                        aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCa                                                                                                                                        >  1:315522/1‑143 (MQ=11)  aGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAt                                                                                                                                       >  1:420318/1‑143 (MQ=31)        tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCt                                                                                                                                 >  2:708283/1‑143 (MQ=35)          tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTACGTTGATGACCCATGATGAACCCTGTTCTATGGCTCCAGATGCCCAACCTGCCCTCATCTCCGGGCCTTCTTCGCACCTTCCATAACGCAGt                                                                                                                               >  1:33781/1‑143 (MQ=11)          tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                               >  1:448645/1‑143 (MQ=37)          tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATATCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                               >  1:490365/1‑143 (MQ=255)            ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAGCATGATCTCATATCAGGCACTTGATCCCACCGCCCATCACGCTATAg                                                                                                                             >  2:399751/1‑143 (MQ=11)                cTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                         <  1:269808/143‑1 (MQ=255)                cTGGTCTGACGGAGCTTACTGCTGAATTCCCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                         <  1:535975/143‑1 (MQ=255)                 tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccacc                                                                                                                        >  2:201057/1‑143 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTACATAACCCTGTAACCACAAGCACAg                                                                                                                 >  2:649042/1‑143 (MQ=14)                            cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCGCATCACAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAgctgt                                                                                                             >  1:535856/1‑139 (MQ=255)                                          ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACCGACaca                                                                                               >  1:156570/1‑141 (MQ=255)                                                  cGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGCTCTTGCGGAACGACAAAGAGAAACa                                                                                       >  2:2752/1‑143 (MQ=255)                                                             aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTg                                                                            >  1:651760/1‑143 (MQ=255)                                                               ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATATCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGGGGAACAGAACCAGATTGAt                                                                          >  2:217203/1‑143 (MQ=255)                                                                tgatgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATg                                                                         >  1:345212/1‑143 (MQ=255)                                                                    gccTCATGGTGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCATCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                     <  2:55979/141‑1 (MQ=255)                                                                    gaCTCATGATGACCCCTGTTCTACGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                     <  2:714879/143‑1 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGAGGCACGACAAAGCGAAACAGAACCAGCATAATGAATTGAg                                                                  >  2:126726/1‑143 (MQ=21)                                                                         atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCt                                                                >  1:643131/1‑143 (MQ=255)                                                                                aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCGATAAGACTGTCGACACAAGAACAGATATTGCGGAACGACAAAGAGAAGCAGAACCAGAACGGTGCATGAAGCAGTCATcc                                                         >  2:76799/1‑143 (MQ=18)                                                                                   cttgtttATTGCTTCAGTTGACAAATATTGTCTCATATCAGGGTCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATGTTGCGTTACGACAAAGAGAAACATTACTAGATTTATGCATTTAGCTTTCATCCTAt                                                      <  2:137769/137‑1 (MQ=255)                                                                                        cTATGGCTCCAGATGACAAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaat                                                 <  1:67985/143‑1 (MQ=255)                                                                                              cTCCAGATGACAAACATGATCTCATATCAGGGGCATGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGCTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAACCTATGAAATCAATTg                                           >  2:665862/1‑143 (MQ=38)                                                                                              cTCCAGACAACAAACCTGATCTCAAATCCGGGACTTGTTCGCCCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTg                                           <  1:138805/143‑1 (MQ=255)                                                                                                  ataTGACAAACATTATCTCATATCAGGGACTTGTTCGCACCATCCATAACGCTGTAGCCAGCAGAACAGATGTTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGt                                       <  1:677838/141‑1 (MQ=255)                                                                                                   gATGACAAACATGATCCCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCTGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                      <  1:659917/143‑1 (MQ=255)                                                                                                      gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                         >  2:164463/1‑89 (MQ=255)                                                                                                      gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                         <  1:164463/89‑1 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                >  1:670287/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                >  1:48482/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                >  2:479014/1‑143 (MQ=255)                                                                                                           aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAt                              <  1:581079/143‑1 (MQ=255)                                                                                                           aCATGATCTCATATCAGCGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAt                              <  1:588242/143‑1 (MQ=255)                                                                                                            cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAACAGTAtt                             >  2:326514/1‑143 (MQ=255)                                                                                                             ataaTCTCAGAGCAGGGACTTGTTCGCACCTGCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTg                            <  1:649042/140‑1 (MQ=255)                                                                                                                aTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCCTCCTATGAAATTAATTGCTGTTAAAAGCATTGGGt                         >  2:676632/1‑143 (MQ=255)                                                                                                                aTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGt                         >  1:175697/1‑143 (MQ=255)                                                                                                                   tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTCAAAAGCATTGCGTACa                      >  2:695894/1‑143 (MQ=255)                                                                                                                      tatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCAGCCTATGAAATTAATTGCTGTTAAAAACATCGGGTACAGaa                   >  2:618935/1‑143 (MQ=255)                                                                                                                        tCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGATCTTTCGTCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                 >  2:620188/1‑143 (MQ=255)                                                                                                                           gggACTTGTCCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc              <  1:23444/143‑1 (MQ=255)                                                                                                                            ggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAGATTAATTGCTGTTAAAAGGAGTGGGTACAGAAGATAcc             >  1:416/1‑143 (MQ=255)                                                                                                                             gACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAccc            <  2:410880/143‑1 (MQ=255)                                                                                                                                  gTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGc       >  1:600477/1‑143 (MQ=255)                                                                                                                                   ctCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCt      <  1:314856/142‑1 (MQ=255)                                                                                                                                      gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCa   >  1:418714/1‑143 (MQ=255)                                                                                                                                       cACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAt  >  2:518720/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                        AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCAT  >  NC_000913/2289972‑2290248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.