Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,992,524 (CGGGC)2→1 coding (1372‑1376/2547 nt) cyaA → adenylate cyclase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3992522 =2 (0.030)48 (0.830) 45/270 0.2 96.1% coding (1370/2547 nt) cyaA adenylate cyclase
?NC_000913 = 3992516 2 (0.030)coding (1364/2547 nt) cyaA adenylate cyclase

ACGGGTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3992670‑3992522
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcccggcGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGACTGGCGGAGA  <  NC_000913/3992516‑3992375
                                                                                                                                                                                                                                                                                                   
ACGGGTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAG                                                                                                                                                      >  1:857969/1‑143
       CGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTCGGCGCGCGGTTATACAGATACCAACCTGAACGGCTAGCCCGGCG                                                                                                                                               >  2:753277/1‑143
        GCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTGTACAGATACCCACCTGAACGGTTAGCCCGGCGG                                                                                                                                              >  1:91520/1‑143
         CGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAGCCTGAACGGTTAGCCCGGCGGg                                                                                                                                             >  2:554825/1‑142
              TCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTCTACAGATACCAACCTGCACGGTTAGCCCGGCGGCcccgc                                                                                                                                        >  1:271328/1‑138
                  CAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGCTGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATCCCAACCTCAACGGTCCGCCCGGCGGCACATAAATA                                                                                                                                    >  2:671275/1‑143
                   AGGCAGTTAAACCATGCCCACGCCCCCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCCGATGATCGACTCAATATTTGGCGCGCGGTTATACAGAACCCAACCTTAACGGTTAGCCCGGCGGCACATAAATAA                                                                                                                                   <  2:898202/143‑1
                                 TGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGCAAGGTTAGCCCCGCGGCGCCGAAATCAAGGCCAGATCCGtg                                                                                                                     >  2:28773/1‑141
                                      ACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGA                                                                                                                <  1:1039795/143‑1
                                       CGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAG                                                                                                               <  2:578232/143‑1
                                       CGCCACCAGGTTAGTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCTGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAG                                                                                                               <  2:130525/143‑1
                                          CACCAGTTTATTCAGGTAACGGTTATATCCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTGTACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGc                                                                                                            >  1:596871/1‑142
                                             CAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTCGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGCGCTCG                                                                                                         >  2:870648/1‑143
                                              AGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGTCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGG                                                                                                        <  1:821688/143‑1
                                               ggttagcCAGGTAACGGTGATATTCCGGCGGCTGATGGCCGATGATCGGCTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGG                                                                                                       <  1:121822/136‑1
                                                         gcAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGC                                                                                             <  1:403588/141‑1
                                                         GTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGC                                                                                             >  1:269839/1‑143
                                                          TAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCG                                                                                            <  1:478194/143‑1
                                                           AACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGG                                                                                           <  1:32548/143‑1
                                                              GGTTATATTCCCGCGGGTGATGGCTGATGATCGACTCAATGTTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTT                                                                                        <  2:746612/143‑1
                                                                TTATATTCCAGTGGCTGATGGCTGATGATCGACTCAATATTTGGCGAGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTA                                                                                      <  1:637062/143‑1
                                                                  ATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATACAGGTCAGATTCGGTTAAGAGCGAACGGGTGAAATCTGCGGGTTTACC                                                                                    >  1:682853/1‑143
                                                                     TTCCAGCGGCTGATGCCTGATGATCGACTCGATAGTTGGCTCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGC                                                                                 <  2:975900/143‑1
                                                                         AGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCA                                                                             >  2:923321/1‑143
                                                                            GGCTGATGGCTGATGATCCACTCAATATTTGGCGCGCGGTTATAGAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAAGTCAGATTCGGTCCCGAGAGAGCGGGGGCCATCTGCCGGTTAATCCGAGTCACct                                                                          >  2:968952/1‑141
                                                                                GATGGCGGATGATCGACTCAATCTTGGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACC                                                                      <  2:613775/143‑1
                                                                                 ATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCT                                                                     >  1:1079583/1‑143
                                                                                      TGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCCCTTTACCTGGTAA                                                                >  1:483751/1‑143
                                                                                      TGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAA                                                                <  2:637125/143‑1
                                                                                           ATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTT                                                           >  1:668421/1‑143
                                                                                               ACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTGATGCTTCAAc                                                       >  1:642046/1‑142
                                                                                                  tcagcTTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGC                                                    <  1:30800/138‑1
                                                                                                      ATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCA                                                <  1:592781/143‑1
                                                                                                           cCGCGCGGTTACACCGATACCAACCCTAACGGTTAGCCCGGCGGCACCTAAATAAAGGTCCGATTCGGTTCCTCGAGATCGGGTGAAATCTGCGGGTTTCCCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAG                                           <  1:28773/142‑1
                                                                                                             GCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGGTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCT                                         >  1:522290/1‑143
                                                                                                               GCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACAGAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTA                                       <  2:651272/143‑1
                                                                                                                 GGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAACTCTGCGGGTTTACCAGCGTCACTTTACCTGGTGATGCTTCAAACGCGGCATACCGCTTACG                                     >  1:366982/1‑143
                                                                                                                   TTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGGTTACCAGCGTCACTTTACCTGGTAATGCGTCAAACGCGGCCTACAGCCGACGCG                                   >  2:645271/1‑143
                                                                                                                      TACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAGTGCTTCAAACGCGGCATCCAGCTTACGCGTCg                                >  1:1022492/1‑142
                                                                                                                      TACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCA                                <  1:181570/143‑1
                                                                                                                       ACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCGAACGCGGCATCCCGCTTACGCGTCAG                               >  1:219245/1‑143
                                                                                                                               CAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTTGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGA                       >  2:455385/1‑143
                                                                                                                                     GAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCT                 >  2:576680/1‑143
                                                                                                                                       ACGGTTAGCCCGGCGGCACATAAATCAAGGTCAGATTCGGTTCCGAGAGATCGGGCGAAATCGGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATGCTGC               >  2:186432/1‑143
                                                                                                                                       ACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATTCTTCAAACGCGGCATACAGCTTACGCCTCAGCAAGCCGATATACTGC               >  2:965408/1‑143
                                                                                                                                       ACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGC               <  1:870648/143‑1
                                                                                                                                         GGTCAGCGCGGCGACACCTAAATTATGGTCAGACTCGGGTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTCGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGG             <  2:30167/143‑1
                                                                                                                                          GTTAGCCCGGCGCCCCATACATAAGGGTCAGCTTCGGTTCCGAGAGATCGGGTGACATCTGCGGGCTTACCAGCGGCCCTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGA            <  2:807630/143‑1
                                                                                                                                           TTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTCACGCGTCAGCACGCCGATCTCCCGCGGcc           >  2:115078/1‑141
                                                                                                                                               CCCGGCGGCACGTGAATAAAGGTCATATTCGGTTCCGAGAGATCGGTTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGACTGGC       <  2:492862/143‑1
                                                                                                                                                CCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGACCGGCG      >  2:315026/1‑143
                                                                                                                                                    CGGCACATAAATAACGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGACTGGCGGAGA  <  1:592628/143‑1
                                                                                                                                                                                                                                                                                                   
ACGGGTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3992670‑3992522
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcccggcGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTGCGGACTGGCGGAGA  <  NC_000913/3992516‑3992375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.