Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,992,524 (CGGGC)2→1 coding (1372‑1376/2547 nt) cyaA → adenylate cyclase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3992522 =2 (0.030)48 (0.830) 45/270 0.2 96.1% coding (1370/2547 nt) cyaA adenylate cyclase
?NC_000913 = 3992516 2 (0.030)coding (1364/2547 nt) cyaA adenylate cyclase

GCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCG  >  NC_000913/3992380‑3992663
                                                                                                                                              |                                                                                                                                             
tctccgccagtccgcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgaccgttatttatgtgccg                                                                                                                                               <  1:592628‑M1/1‑1 (MQ=255)
    cgccggtccgcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCgg                                                                                                                                           >  2:315026‑M1/139‑143 (MQ=255)
     gccagtccgcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcaaccgatctctcggaaccgaatatgacctttattcacgtgccGCCggg                                                                                                                                          <  2:492862‑M1/6‑1 (MQ=255)
         ggccgcgggagatcggcgtgctgacgcgtgagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTaa                                                                                                                                      >  2:115078‑M1/134‑143 (MQ=255)
          tccgcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagggccgctggtaagcccgcagatgtcacccgatctctcggaaccgaagctgacccttatgtatggggcGCCGGGCTAAc                                                                                                                                     <  2:807630‑M1/11‑1 (MQ=255)
           ccgcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattacgaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggacccgagtctgaccataatttaggtgtcGCCGCGCTGAcc                                                                                                                                    <  2:30167‑M1/12‑1 (MQ=255)
             gcagtatatcggcttgctgaggcgtaagctgtatgccgcgtttgaagaattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGt                                                                                                                                  >  2:965408‑M1/130‑143 (MQ=255)
             gcaggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGt                                                                                                                                  <  1:870648‑M1/14‑1 (MQ=255)
             gcagcatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgccgatttcgcccgatctctcggaaccgaatctgaccttgatttatgtgccGCCGGGCTAACCGt                                                                                                                                  >  2:186432‑M1/130‑143 (MQ=255)
               aggatatcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTc                                                                                                                                >  2:576680‑M1/128‑143 (MQ=255)
                     tcggcgtgctgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccaaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTg                                                                                                                          >  2:455385‑M1/122‑143 (MQ=255)
                             ctgacgcgtaagcgggatgccgcgttcgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGt                                                                                                                  >  1:219245‑M1/114‑143 (MQ=255)
                              tgacgcgtaagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGta                                                                                                                 <  1:181570‑M1/31‑1 (MQ=255)
                              cgacgcgtaagctggatgccgcgtttgaagcactaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGta                                                                                                                 >  1:1022492‑M1/113‑143 (MQ=255)
                                 cgcgtcggctgtaggccgcgtttgacgcattaccaggtaaagtgacgctggtaaccccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATaa                                                                                                              >  2:645271‑M1/110‑143 (MQ=255)
                                   cgtaagcggtatgccgcgtttgaagcatcaccaggtaaagtgacgctggtaaacccgcagagttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAAcc                                                                                                            >  1:366982‑M1/108‑143 (MQ=255)
                                     taagctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttctgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgc                                                                                                          <  2:651272‑M1/38‑1 (MQ=255)
                                       agctgtatgccgcgtttgaagcattaccaggtaaagtgacgctggtaaccccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgcgc                                                                                                        >  1:522290‑M1/104‑143 (MQ=255)
                                         ctgtatgccgcgtttgaagcattaccaggtaaagtgacgctgggaaacccgcagatttcacccgatctcgaggaaccgaatcggacctttatttaggtgccGCCGGGCTAACCGTTAGGGTTGGTATCGGTGTAAccgcgcgg                                                                                                      <  1:28773‑M1/42‑2 (MQ=255)
                                              tgccgcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAat                                                                                                 <  1:592781‑M1/47‑1 (MQ=255)
                                                  gcgtttgaagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCgcgccaaagctga                                                                                             <  1:30800‑M1/51‑6 (MQ=255)
                                                   caacctttACCATCTAAATCGACTCTTCTAATCCCCCATATTTCACCCGTTCTCTCGTAACCGAATCTGACCTTTATTTATGTGCCTCCG‑‑‑C‑‑TCTAACCTTTCATTTTGGTATCTTTATAACCTCTCGCCAAATATTGAGTCGa                                                                                       <  2:800062/137‑1 (MQ=255)
                                                     gttgaagcatcaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGt                                                                                          >  1:642046‑M1/90‑143 (MQ=255)
                                                         aagcattaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGat                                                                                      >  1:668421‑M1/86‑143 (MQ=255)
                                                              ttaccaggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGAtcatca                                                                                 <  2:637125‑M1/63‑1 (MQ=255)
                                                              ttaccaggtaaagggacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGAtcatca                                                                                 >  1:483751‑M1/81‑143 (MQ=255)
                                                                   aggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccat                                                                            >  1:1079583‑M1/76‑143 (MQ=255)
                                                                    ggtaaagtgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCCAAGATTGAGTCGATcatccgccatc                                                                           <  2:613775‑M1/69‑1 (MQ=255)
                                                                        aggtgactcggattaaccggcagatggcccccgctctctcgggaccgaatctgacttttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTCTATAACCGCGCGCCAAATATTGAGTGGATCatcagccatcagcc                                                                       >  2:968952‑M1/71‑143 (MQ=255)
                                                                           tgacgctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCt                                                                    >  2:923321‑M1/68‑143 (MQ=255)
                                                                               gctggtaaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGAGCCAACTATCGAGTCGATCATCAGGCATCAGCCGCTGGaa                                                                <  2:975900‑M1/80‑1 (MQ=255)
                                                                                  ggtaaacccgcagatttcacccgttcgctcttaaccgaatctgacctgtatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAatat                                                             >  1:682853‑M1/61‑143 (MQ=255)
                                                                                    taaacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCTCGCCAAATATTGAGTCGATCATCAGCCATCAGCCACTGGAATATaa                                                           <  1:637062‑M1/85‑1 (MQ=255)
                                                                                      aacccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAACATTGAGTCGATCATCAGCCATCACCCGCGGGAATATAAcc                                                         <  2:746612‑M1/87‑1 (MQ=255)
                                                                                         ccgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGtt                                                      <  1:32548‑M1/90‑1 (MQ=255)
                                                                                          cgcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTa                                                     <  1:478194‑M1/91‑1 (MQ=255)
                                                                                           tttCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTACGTGC‑‑CC‑‑‑CCGGGCTCACCGTTCAGGCTGGTATCTGTATAACCGCGCGCCAACAATTGCGCCGATCATCTGCCACCCGCCGCTGGGCTAAAACCCttctccgac                                               >  2:194023/1‑136 (MQ=255)
                                                                                           gcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACcgttgc                                                    <  1:403588‑M1/92‑3 (MQ=255)
                                                                                           gcagatttcacccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTAc                                                    >  1:269839‑M1/52‑143 (MQ=255)
                                                                                                     cccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGCCGATCATCGGCCATCAGCCGCCGGAATATCACCgttacctggctaacc                                          <  1:121822‑M1/102‑8 (MQ=255)
                                                                                                      ccgatctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGACATCAGCCGCTGGAATATAACCGTTACCTGAATAAACt                                         <  1:821688‑M1/103‑1 (MQ=255)
                                                                                                       cgagcgctcggaaccgaatctgacctttatttatgtgccGCCGGGCGAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACtg                                        >  2:870648‑M1/40‑143 (MQ=255)
                                                                                                          gctctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTACAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGGATATAACCGTTACCTGAATAAACtggtg                                     >  1:596871‑M1/37‑143 (MQ=255)
                                                                                                             ctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCg                                  <  2:578232‑M1/110‑1 (MQ=255)
                                                                                                             ctcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACAGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGACTAACCTGGTGGCg                                  <  2:130525‑M1/110‑1 (MQ=255)
                                                                                                              tcggaaccgaatctgacctttatttatgtgccGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGt                                 <  1:1039795‑M1/111‑1 (MQ=255)
                                                                                                                   cacggatctggccttgatttcggcgccGCGGGGCTAACCTTGCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCa                            >  2:28773‑M1/28‑143 (MQ=255)
                                                                                                                                 ttatttatgtgccGCCGGGCTAACCGTTAAGGTTGGGTTCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCGGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGGGGCGTGGGCATGGTTTAACTGCct              <  2:898202‑M1/130‑1 (MQ=255)
                                                                                                                                  tatttatgtgccGCCGGGCGGACCGTTGAGGTTGGGATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCAGCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCctg             >  2:671275‑M1/13‑143 (MQ=255)
                                                                                                                                      gcggggccGCCGGGCTAACCGTGCAGGTTGGTATCTGTAGAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGa         >  1:271328‑M1/9‑143 (MQ=255)
                                                                                                                                           cccGCCGGGCTAACCGTTCAGGCTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTcg    >  2:554825‑M1/4‑143 (MQ=255)
                                                                                                                                            ccGCCGGGCTAACCGTTCAGGTGGGTATCTGTACAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTcgc   >  1:91520‑M1/3‑143 (MQ=255)
                                                                                                                                             cGCCGGGCTAGCCGTTCAGGTTGGTATCTGTATAACCGCGCGCCGAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTcgcg  >  2:753277‑M1/2‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTGACCTCGCG  >  NC_000913/3992380‑3992663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.