Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	33 [0]	[0] 34	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	33 (0.860)	30/284	0.3	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGT  >  NC_000913/4001510‑4001792                                                                                                                                             |                                                                                                                                               tatggactgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagccttgcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatctc                                                                                                                                              <  1:141410‑M2/3‑1 (MQ=255)     gattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttc                                                                                                                                          <  1:431676‑M2/7‑1 (MQ=255)             ttggctaaccggacgacggcgagcgacggcgggtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                                  <  2:103741‑M2/15‑1 (MQ=255)                  gcaccggacgaccacgagcgactgcgcgtaccacctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAg                                                                                                                             <  1:181028‑M2/20‑1 (MQ=255)                  gaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAg                                                                                                                             <  1:678886‑M2/20‑1 (MQ=255)                         acgacgacgagcgactgcgcgtacaatctgaacttggccagggcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCCCCTTCTTCTTCTTTGAAGATGCGGa                                                                                                                      <  1:454278‑M2/27‑1 (MQ=255)                              gacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                                 >  1:347527‑M2/112‑143 (MQ=255)                                   gcgacggcgcgtacaagataaacggtgccagatcctggcaacccgccctgaacaggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCGTCTTCTTCTTTGAAGATGCGGAAGATCACGcc                                                                                                            <  2:276962‑M2/37‑1 (MQ=255)                                    cgcctgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacggttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCg                                                                                                           <  1:271064‑M2/38‑1 (MQ=255)                                     gactgcgcgtacaagctgaacgtggccggagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCgg                                                                                                          <  2:293625‑M2/39‑1 (MQ=255)                                                   tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTgg                                                                                            >  2:214611‑M2/91‑143 (MQ=255)                                                         cttggcccgagcctggcaaccctcccggaacgcgaagacatcgaagcatcggcacctttctttgaagacgacgacggcctgcatCTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTa                                                                                      <  2:254647‑M2/59‑1 (MQ=255)                                                         ctgggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTa                                                                                      <  2:243967‑M2/59‑1 (MQ=255)                                                          ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGcatttcc                                                                                     >  2:380378‑M2/84‑141 (MQ=255)                                                                agagcctggcaacccgcccgaaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCg                                                                               <  2:262078‑M2/66‑1 (MQ=255)                                                                 gagcctggcagcgcgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCTGTAACTCCACTGTGGCATTTACCATCCGt                                                                              <  2:85106‑M2/67‑1 (MQ=255)                                                                      ggtcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcagCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATgg                                                                         <  2:13882‑M2/72‑1 (MQ=255)                                                                         caacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGGAACTCCACTGTGGCATTTACCATCCGTGATGgtcg                                                                      >  1:277307‑M2/69‑143 (MQ=255)                                                                          aacccgcccggaactggaagacatcgaagcatcggcacgttacacagaagataactacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcgt                                                                     <  1:693521‑M2/76‑1 (MQ=255)                                                                               gcccggaactggaagacctcgacgcatcggcacgtctctttgaaggcgacgacggcctgcatCTCCTTATTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCATCTGtt                                                                <  1:273980‑M2/81‑1 (MQ=255)                                                                               gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCATCTGtt                                                                <  1:640442‑M2/81‑1 (MQ=255)                                                                                       ctggaagacagcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGTTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                        <  1:292209‑M2/89‑1 (MQ=255)                                                                                          gaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCCTCCGGGATGGTCGGCTGTTTACTCTgcgtg                                                     >  1:613719‑M2/52‑143 (MQ=255)                                                                                               cagcgaagcagcggcacgtttcttcgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgt                                                <  1:190207‑M2/97‑1 (MQ=255)                                                                                                atcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTctgcgtgagcgcg                                               >  1:278003‑M2/46‑141 (MQ=255)                                                                                                         tcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGATCGTGAAGTGCCCg                                      >  1:532475‑M2/37‑143 (MQ=255)                                                                                                          cggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGc                                     >  1:666582‑M2/36‑143 (MQ=255)                                                                                                            gcacttttctttgaagactacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCtt                                   <  2:432900‑M2/110‑1 (MQ=255)                                                                                                              acgtttcttggcagacgccgccggccggcacCTCCTTCTTCTTCTTTCAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCtttt                                 <  1:518600‑M2/112‑1 (MQ=255)                                                                                                                       tgaagacgacgacggccggcgtCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTGACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTGTTCGTCTGTAt                        <  2:591259‑M2/121‑1 (MQ=255)                                                                                                                           gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa                    <  1:54097‑M2/125‑1 (MQ=255)                                                                                                                              gccgttggcttgcatCTCCTTCTGTGTCTGTGGAGATGCGGAAGAGCACGCCGGTAACTCCACTGTGGCATTTTCCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGc                 <  1:570790‑M2/128‑1 (MQ=255)                                                                                                                                cgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGcgg               >  2:267810‑M2/14‑142 (MQ=255)                                                                                                                                             cTCCTTCTTCTTCTGTCATGATGCGCACGTGTACGCCGGTAACTCCACTGTGGCATTTCCCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGt  <  2:601751/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGT  >  NC_000913/4001510‑4001792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑