| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 3224522 = | 46 (1.250) | 3 (0.080) | 3/276 | 5.3 | 6.4% | coding (1890/3093 nt) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
| ? | NC_000913 | 3224536 = | 43 (1.200) | coding (1904/3093 nt) | ebgA | evolved beta‑D‑galactosidase, alpha subunit | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGG > NC_000913/3224382‑3224661 | tcggATCAGCCGCCGGGGCCGGGCTTGAAAGAGTACAAACAGGTTATCGCGCGGGTAAAAATCCACGCGCGGGAGCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCa < 1:215899/140‑1 (MQ=255)tCCGAGCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGATTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCa < 1:136702/143‑1 (MQ=255) gATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGc > 2:564330/1‑143 (MQ=255) ggACGCCGGGACGGGGCGTGAAGGAGTACATACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagag < 2:75269/142‑1 (MQ=255) aGACGCCGGGACCGGGCCTGAAAGAGTACAGACAGGTTATCGCGCCGGTAAACATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagag < 1:609543/143‑1 (MQ=255) aGACGCCGGGACCGGGCCTGAAAGAGTACACACAGGTTATCACGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCatag > 1:530265/1‑143 (MQ=255) ggACCGGGCCGGAAAGGGTGGCACAAGGTTAGCGCGGCGGTAAAACTCCAGACGCGGGATCTGAGTCGCCTGGAGTTGAAAGTCGAAAATAGACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcgc < 2:656249/143‑1 (MQ=255) ggtccTGAAAGAGTACGAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg < 2:608860/140‑1 (MQ=255) gggCCTGAACGAGTACAAACAGTTTATCGAGCAATCCACAATCCACTCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg < 2:295351/143‑1 (MQ=255) cccgAAAGAGTACTACCAGGTTATCGCGCCGCTAAGTATCCACGCGCGTGTGCTGACTCGCGGCGAGTGCAAACTCGAAAATCCACCTTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg < 2:79998/140‑1 (MQ=255) ccTGAAAGAGTACAAGCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTGGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg < 2:147385/143‑1 (MQ=255) cTGAAAGAGTACAAACAGGTTAGCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGa < 1:364875/143‑1 (MQ=255) gAAAGAGTACAAACAGGTTATGGCGCCGGTAAAAAGCCACGCGCGGGATCGGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaaa < 1:347111/143‑1 (MQ=255) gAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaac > 1:621348/1‑142 (MQ=255) aaGAGTACAAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACg < 2:343776/143‑1 (MQ=255) aaCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCCGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTAGAAGACTACGCCCTGCACGCCGAGGCGCGCGCAGAAGGTGAAAGGCCGGCGACg > 2:45068/1‑143 (MQ=255) gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGCGTTGAACGTCGAAACTAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCCACccagcg > 2:605739/1‑142 (MQ=255) tATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAACATAAACTGTGGGTTACCACGCTTGATGACTACACCCTGCAAGGAGAGGGGACCCCCCGAGGCGAAACGCTCGCGGCGCAGCAGa > 2:652501/1‑143 (MQ=255) aTCGCGTCGGTAAAAATCCACGCGCGGGCTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGAt < 2:274955/143‑1 (MQ=255) cgcCGGTAAAAATCCACGCGCGGGTTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTaaa < 1:170488/143‑1 (MQ=255) acgcaggcgcgGCACGTGGCTCGAGGGGGGTTGAAGGGCGAAAATAAGCTGTGGTTTACCACGCTTGATGACTACCCCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGt < 2:136206/137‑1 (MQ=255) cgcgcgGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTgcgc < 1:232549/143‑1 (MQ=255) gctCTGACTCGCGGCGAGGTGAAAGTCGAGTATACACTCGGGCGTACCGCGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg < 2:137839/141‑1 (MQ=255) gATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg < 1:564330/143‑1 (MQ=255) tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCGCAGCGAAGccc > 1:356451/1‑143 (MQ=255) tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGccc > 1:552630/1‑143 (MQ=255) cggcgAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCACAGCGAAccccccc > 2:28819/1‑142 (MQ=255) tCGAAACTAAACTGTGGTTTACTACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTg < 2:571019/143‑1 (MQ=255) cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc < 1:374693/143‑1 (MQ=255) cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGACCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc > 1:155447/1‑143 (MQ=255) gtaagtAAACTGTGCTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGcc < 1:119479/138‑1 (MQ=255) tgtgGGTGACCGCTCTTGGTGACTATACCCTGCGCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGAc < 2:602125/143‑1 (MQ=255) cTTGATTACTACACCCTGCACGCATAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGtt < 1:345342/143‑1 (MQ=255) gaggaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCt < 2:149032/140‑1 (MQ=255) gaCTACACCCTGCTCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGGTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa < 1:593409/143‑1 (MQ=255) gaCTACACCCTGCACGCATAGGTGCGCGCCGAAGGCGCAACGCTCGCGACGCAGCAGATGAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCTCCTTGCAGATCTCGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa < 2:46793/143‑1 (MQ=255) gaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CCGCTGGACGCCCGCGACGCGTTCCTCaa > 1:575095/1‑143 (MQ=255) aCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGAACACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGCTCCTCAAc > 2:131105/1‑143 (MQ=255) cTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACa < 1:42899/143‑1 (MQ=255) acacCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCCAACAGCGAAGCCCACTTATAAAAC‑CATTAACGTCAGCTGGACGCCCGCGAAGCGTTCCTCAACAtt > 1:488098/1‑143 (MQ=255) cacCCTGCACGCAGAGGTGCGCGCCGAAGGCAAGAATCCCGCTGCGCAGCAGATTAAACTGCGCGGCGATGCGCCGAACAGCGCACCCCCCTCGCAGATCACGCTGCCC‑CAGCCGCACGCCCGCCAAGCGATCCTCAACCTTa > 2:171377/1‑143 (MQ=255) acCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAACCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGCAGCGGTCCTCAACAttgc > 1:294303/1‑141 (MQ=255) ccccGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCGCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 1:453768/143‑1 (MQ=255) cccTGCACGCAGAGGTGCGCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGAGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 2:202055/143‑1 (MQ=255) cccTCCCCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg < 1:17241/143‑1 (MQ=255) ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAGCCGTTCCTCAACATTACgg > 1:675969/1‑143 (MQ=255) ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACgg < 2:494082/143‑1 (MQ=255) | TCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGG > NC_000913/3224382‑3224661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |