New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3224522 =	46 (1.250)	3 (0.080)	3/276	5.3	6.4%	coding (1890/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
	?	NC_000913	3224536 =	43 (1.200)					coding (1904/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224397‑3224675                                                                                                                                            |                                                                                                                                             ggACCGGGCCGGAAAGGGTGGCACAAGGTTAGCGCGGCGGTAAAACTCCAGACGCGGGATCTGAGTCGCCTGGAGTTGAAAGTCGAAAATAGACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcgc                                                                                                                                           <  2:656249/143‑1 (MQ=255)      ggtccTGAAAGAGTACGAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg                                                                                                                                      <  2:608860/140‑1 (MQ=255)      gggCCTGAACGAGTACAAACAGTTTATCGAGCAATCCACAATCCACTCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg                                                                                                                                      <  2:295351/143‑1 (MQ=255)         cccgAAAGAGTACTACCAGGTTATCGCGCCGCTAAGTATCCACGCGCGTGTGCTGACTCGCGGCGAGTGCAAACTCGAAAATCCACCTTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg                                                                                                                                   <  2:79998/140‑1 (MQ=255)         ccTGAAAGAGTACAAGCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTGGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg                                                                                                                                   <  2:147385/143‑1 (MQ=255)          cTGAAAGAGTACAAACAGGTTAGCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGa                                                                                                                                  <  1:364875/143‑1 (MQ=255)            gAAAGAGTACAAACAGGTTATGGCGCCGGTAAAAAGCCACGCGCGGGATCGGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaaa                                                                                                                                <  1:347111/143‑1 (MQ=255)            gAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaac                                                                                                                                >  1:621348/1‑142 (MQ=255)              aaGAGTACAAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACg                                                                                                                              <  2:343776/143‑1 (MQ=255)                       aaCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCCGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTAGAAGACTACGCCCTGCACGCCGAGGCGCGCGCAGAAGGTGAAAGGCCGGCGACg                                                                                                                     >  2:45068/1‑143 (MQ=255)                            gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGCGTTGAACGTCGAAACTAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCCACccagcg                                                                                                                >  2:605739/1‑142 (MQ=255)                              tATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAACATAAACTGTGGGTTACCACGCTTGATGACTACACCCTGCAAGGAGAGGGGACCCCCCGAGGCGAAACGCTCGCGGCGCAGCAGa                                                                                                              >  2:652501/1‑143 (MQ=255)                               aTCGCGTCGGTAAAAATCCACGCGCGGGCTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGAt                                                                                                             <  2:274955/143‑1 (MQ=255)                                   cgcCGGTAAAAATCCACGCGCGGGTTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTaaa                                                                                                         <  1:170488/143‑1 (MQ=255)                                              acgcaggcgcgGCACGTGGCTCGAGGGGGGTTGAAGGGCGAAAATAAGCTGTGGTTTACCACGCTTGATGACTACCCCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGt                                                                                              <  2:136206/137‑1 (MQ=255)                                                   cgcgcgGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTgcgc                                                                                         <  1:232549/143‑1 (MQ=255)                                                          gctCTGACTCGCGGCGAGGTGAAAGTCGAGTATACACTCGGGCGTACCGCGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg                                                                                  <  2:137839/141‑1 (MQ=255)                                                          gATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg                                                                                  <  1:564330/143‑1 (MQ=255)                                                                  tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCGCAGCGAAGccc                                                                          >  1:356451/1‑143 (MQ=255)                                                                  tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGccc                                                                          >  1:552630/1‑143 (MQ=255)                                                                     cggcgAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCACAGCGAAccccccc                                                                       >  2:28819/1‑142 (MQ=255)                                                                                   tCGAAACTAAACTGTGGTTTACTACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTg                                                         <  2:571019/143‑1 (MQ=255)                                                                                    cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc                                                        <  1:374693/143‑1 (MQ=255)                                                                                    cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGACCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc                                                        >  1:155447/1‑143 (MQ=255)                                                                                     gtaagtAAACTGTGCTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGcc                                                       <  1:119479/138‑1 (MQ=255)                                                                                               tgtgGGTGACCGCTCTTGGTGACTATACCCTGCGCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGAc                                            <  2:602125/143‑1 (MQ=255)                                                                                                             cTTGATTACTACACCCTGCACGCATAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGtt                              <  1:345342/143‑1 (MQ=255)                                                                                                                gaggaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCt                           <  2:149032/140‑1 (MQ=255)                                                                                                                   gaCTACACCCTGCTCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGGTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa                        <  1:593409/143‑1 (MQ=255)                                                                                                                   gaCTACACCCTGCACGCATAGGTGCGCGCCGAAGGCGCAACGCTCGCGACGCAGCAGATGAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCTCCTTGCAGATCTCGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa                        <  2:46793/143‑1 (MQ=255)                                                                                                                   gaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CCGCTGGACGCCCGCGACGCGTTCCTCaa                        >  1:575095/1‑143 (MQ=255)                                                                                                                    aCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGAACACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGCTCCTCAAc                       >  2:131105/1‑143 (MQ=255)                                                                                                                     cTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACa                      <  1:42899/143‑1 (MQ=255)                                                                                                                       acacCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCCAACAGCGAAGCCCACTTATAAAAC‑CATTAACGTCAGCTGGACGCCCGCGAAGCGTTCCTCAACAtt                    >  1:488098/1‑143 (MQ=255)                                                                                                                        cacCCTGCACGCAGAGGTGCGCGCCGAAGGCAAGAATCCCGCTGCGCAGCAGATTAAACTGCGCGGCGATGCGCCGAACAGCGCACCCCCCTCGCAGATCACGCTGCCC‑CAGCCGCACGCCCGCCAAGCGATCCTCAACCTTa                   >  2:171377/1‑143 (MQ=255)                                                                                                                         acCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAACCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGCAGCGGTCCTCAACAttgc                  >  1:294303/1‑141 (MQ=255)                                                                                                                          ccccGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCGCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg                 <  1:453768/143‑1 (MQ=255)                                                                                                                          cccTGCACGCAGAGGTGCGCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGAGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg                 <  2:202055/143‑1 (MQ=255)                                                                                                                          cccTCCCCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg                 <  1:17241/143‑1 (MQ=255)                                                                                                                           ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAGCCGTTCCTCAACATTACgg                >  1:675969/1‑143 (MQ=255)                                                                                                                           ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACgg                <  2:494082/143‑1 (MQ=255)                                                                                                                               cACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGAc            >  2:550867/1‑143 (MQ=255)                                                                                                                                  gCAGAGGTGCGCGCCGAAGGTGAGACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCaa         <  2:87874/143‑1 (MQ=255)                                                                                                                                   cggagGTGCGCGCCGAAGGGGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCaaa        <  1:92088/141‑1 (MQ=255)                                                                                                                                         tGCGCGTCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTcc  <  2:597094/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             GGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCC  >  NC_000913/3224397‑3224675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.