Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,491,904	IS186 (+) +3 bp	intergenic (+54/+16)	ydcA → / ← hokB	putative periplasmic protein/toxic polypeptide, small

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 609351	NA (NA)	40 (0.860)	35/274	0.3	100%	noncoding (1345/1345 nt)	IS186	repeat region
	?	NC_000913	1491904 =	0 (0.000)					intergenic (+54/+18)	ydcA/hokB	putative periplasmic protein/toxic polypeptide, small
*	?	NC_000913	= 1491906	0 (0.000)	56 (1.200)	53/274	0.0	100%	intergenic (+56/+16)	ydcA/hokB	putative periplasmic protein/toxic polypeptide, small
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region

GGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACT  >  NC_000913/609210‑609486                                                                                                                                               |                                                                                                                                        ggAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTACGGGAg                                                                                                                                        >  1:193685/1‑143 (MQ=11)       tACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATcc                                                                                                                                  <  2:680417/143‑1 (MQ=35)         cAGGTGGCCATTCGTGCGACACTATCCCTGACAGCCCACAAACCGCAATTGCAGAACGCGAGGCATCGTCTTAACGAGCCACCGAGGCGTCCCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATcccc                                                                                                                                <  1:393035/143‑1 (MQ=11)           ggTGGCCATTCGTGGGACAGTATCCCGGACAGCCTACAAGACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGc                                                                                                                              <  2:837619/143‑1 (MQ=255)            gTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGACGCATTCTTCAGATGGTT‑CAACCCTGACGTTAGCGCTTATGGGCGTAACCCCAGCa                                                                                                                             >  2:383814/1‑143 (MQ=11)                  atgcGTGGGACAGTATCCCTGACAGCTTAAATGCCCGAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCg                                                                                                                       <  1:681733/140‑1 (MQ=14)                     cGTGGGACAGTATCCCTGACAGCCTACACAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGtt                                                                                                                    <  1:180946/143‑1 (MQ=255)                      gTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCTTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAGGTTAGCGCTTATGGGAGTGATCCCCGCATATCCGGtag                                                                                                                   >  1:36608/1‑141 (MQ=255)                        gggACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGCGGCACCGAGCCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTCGCGCTTATGGGAGTCATCCCCGCATATCCGGtcgcc                                                                                                                 >  1:749287/1‑139 (MQ=14)                         ggACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtca                                                                                                                <  1:522117/143‑1 (MQ=255)                                   ccTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCAGTCTTCAGATGGTT‑CAACCCTTCAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCTGGATg                                                                                                      <  2:730559/143‑1 (MQ=255)                                    cTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGTGTGTAATCACCGCATATCCGGTTGTCAGGTCAGGATgg                                                                                                     >  1:784102/1‑143 (MQ=255)                                           ccTACAAAACGCAATTGAAGAACGCGAAGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCa                                                                                              >  2:224650/1‑143 (MQ=255)                                            cTACAAAAGGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGGT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATCGTGAGGCAc                                                                                             <  2:152340/143‑1 (MQ=255)                                             tACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCAcc                                                                                            >  2:412017/1‑143 (MQ=255)                                               cAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCCGGATGGTAAGGCACCTg                                                                                          >  1:394916/1‑143 (MQ=255)                                                      ccatcgaagaaCGCGAGGCATCGTCGTAACGAGGCACCGAGGCGTCGCTTTCCTCACAAGATT‑CAACCCTTAAGGTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTaca                                                                                   <  1:874006/138‑1 (MQ=21)                                                         ttGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAACCCCCGCATCTCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACtt                                                                                >  1:513264/1‑143 (MQ=255)                                                           gaagaaCGCGAGGCATCTTCGTAACCAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGCTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGTTCAGGATGGTAAGGCACCTGCTTTACACTTTc                                                                              <  2:455372/143‑1 (MQ=25)                                                            aagaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCCGGCTGGTAAGGCACCTGCTTTACACTTTCg                                                                             >  1:851161/1‑143 (MQ=255)                                                             agaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGCCAGGTCAGGACGGTAAGGCACCCGCTTCACACTTTCGc                                                                            >  2:194365/1‑143 (MQ=255)                                                                  gcgAGGCATCGTCTTACGTAGGCACCGAGGTGTCGCATGCCTCAGATGGTT‑CACCCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTg                                                                       <  2:502352/143‑1 (MQ=25)                                                                  gcgAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTg                                                                       <  1:462133/143‑1 (MQ=255)                                                                    gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGCAAGGCACCTGCTTTACACTTTCGCCCGTGGt                                                                     >  2:870769/1‑143 (MQ=255)                                                                      ggccgcgtcgtAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCGTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCa                                                                   <  2:331081/138‑1 (MQ=255)                                                                            gTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATg                                                             <  1:423959/143‑1 (MQ=255)                                                                               ttAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACGTTCGGCCGTGGAGAGTGATGGCt                                                          >  2:539953/1‑143 (MQ=255)                                                                                    gAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGAGCATATGGGAGTAATACCTGCATATGCGGTTGTCAGGTCAGGATGGTAGGGCACCTGCTTTGCACGCTCGGCCGTGGTCAGGGCTGGATGAggg                                                     >  1:577535/1‑143 (MQ=21)                                                                                     aGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCCGTGATGGCTGCGGGc                                                    >  2:730774/1‑143 (MQ=255)                                                                                         aCCGAGGCGTCGCATTCTTCAGATGGTTGCAAACC‑CAACCCAGCGCCTATGGGAGTAATCCCCGCATATCCGGTTGTCAGTTCAGGATGGTAACGCACCCGCTTTACACTTTCGCACGTGGTCAGTGATGGCTGCGGGCCaac                                                >  2:629332/1‑142 (MQ=255)                                                                                         aCCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGCGAGCGCTGCGGGCgcct                                                >  1:781822/1‑140 (MQ=255)                                                                                         aCCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGATCAAGATGGTAAGGCACCTCCCTTACACTTTCGCCCGTGGTCCGTGATGGATGGGGGCGAAt                                                >  2:99941/1‑143 (MQ=37)                                                                                             ctgcttCGCAGTCTTCAGATGGTT‑CAACCTTTATGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTa                                            <  2:829869/138‑1 (MQ=255)                                                                                             atgCGTCGCATTCTGCAGATGGTT‑CAACCCTTAGGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGGACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTa                                            <  2:323876/141‑1 (MQ=255)                                                                                                    gCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTAAGGATGGTAAGACACCTGCTTTACACTTTCGCCCGTGGTCAGTGAGGGCTGCGGGGGACTCGTAGCAGAtg                                     >  2:379502/1‑143 (MQ=38)                                                                                                          ttcAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCa                               >  1:347946/1‑143 (MQ=255)                                                                                                           tcAGATGGTT‑CAACCCTTAGGTTAGCGCTTATGCGAGTAATCCCCGCATCTCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACCCTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCaa                              <  1:819173/143‑1 (MQ=255)                                                                                                           tcAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGc                                                                                                                              <  1:668329/47‑1 (MQ=255)                                                                                                           tcAGATGGTT‑CAACCCTTAAGTTAGCGCGTATGGGAGTAATCCCCGc                                                                                                                              >  2:668329/1‑47 (MQ=255)                                                                                                               aTGGTT‑CAACCCTTAAGTTAGCGCTTCTGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTGTCGCCAGTGGTCAGTCCTGGCTGCGTGCGCATCGTACCAGCTGGTGGCAAtgag                          >  2:809538/1‑140 (MQ=255)                                                                                                                tGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAAt                         >  2:599127/1‑143 (MQ=255)                                                                                                                 ggTT‑CAGCCCTTTAGTTAGCGCTCATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGGTAGTATGGCTGCCGCTTCACCCGTTCGCCCGTTGTCATTGATGGCTGCGGGCGCATCGTACCAGATGTTGTCAATTAATc                        <  1:643709/143‑1 (MQ=255)                                                                                                                        ccccGTAAGTTAGCGCGTATGGGAGTAACCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACGCTTTCGCCCGTGGTCAGCGATCCGTGTGTGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTg                  <  2:62593/142‑1 (MQ=255)                                                                                                                        aCCCTGAAGTGAGCGCGGAGGGGAGTAATGCCCGCATATCCGGTGGTCAGGGCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTg                  <  1:470030/143‑1 (MQ=255)                                                                                                                            ttAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGTCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGcac              >  2:573295/1‑143 (MQ=255)                                                                                                                              ggggtAGCGGGTATGGGAGTAAGCCCCGCGTATGCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAg            <  2:749287/139‑1 (MQ=255)                                                                                                                               gttgaGCGCTTATGGGATTAATCCCCGCATCTCCGGTTGTCAGGTCAGGATGGCACGGCACCTGCTTTACTCTCTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATTGTACCAGATGTTTTCAATTAATCGTGTTGGCACAGc           <  2:551529/139‑1 (MQ=255)                                                                                                                                gTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCg          <  1:501708/143‑1 (MQ=255)                                                                                                                                 tgaGCGCTTCTGGGAGTAATCCCCGCATAGCCGGTTGTCAGGTCAGGATGGGAAGGCACCTGCTTTACACTTTCGCCCGTGGTGAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGt         <  1:877242/141‑1 (MQ=255)                                                                                                                                  tAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGtt        <  1:461315/143‑1 (MQ=255)                                                                                                                                   ggcgtgtATGGGAGTAAGCCCCGCATGTCCGGTTGGCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTa       <  2:794677/137‑1 (MQ=255)                                                                                                                                    gcgcTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTAt      >  2:606804/1‑143 (MQ=255)                                                                                                                                       cTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGAc   >  1:804480/1‑143 (MQ=255)                                                                                                                                       cTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGAc   >  1:824696/1‑143 (MQ=255)                                                                                                                                        ttATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACt  >  1:392782/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                        GGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTT‑CAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACT  >  NC_000913/609210‑609486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.