Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	153,986	IS5 (–) +4 bp	coding (1438‑1441/2598 nt)	htrE ←	putative outer membrane usher protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	153986 =	0 (0.000)	89 (1.860)	50/282	0.0	100%	coding (1441/2598 nt)	htrE	putative outer membrane usher protein
	?	NC_000913	= 275149	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 153989	0 (0.000)	35 (0.730)	26/282	1.0	100%	coding (1438/2598 nt)	htrE	putative outer membrane usher protein
	?	NC_000913	273955 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTT  >  NC_000913/275011‑275289                                                                                                                                           |                                                                                                                                             aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAg                                                                                                                                          <  1:208507/143‑1 (MQ=32)  aaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAgg                                                                                                                                         <  1:301632/143‑1 (MQ=33)   actctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCATGGACTTGTTCGCACCTTCCGGAGGc                                                                                                                                        <  2:242815/141‑1 (MQ=21)       tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGCTa                                                                                                                                    >  1:131011/1‑143 (MQ=25)        ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAt                                                                                                                                   >  1:142718/1‑143 (MQ=38)               ggggtggcGGGGCTGACTGCTGAATTCACTGGCGGCGAAGGTAAGTTGATGACTCATGATGACCCCTGTTCTATGGCTCCAGATGCCAATCATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTgg                                                                                                                            <  1:492217/136‑1 (MQ=14)                gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggc                                                                                                                           >  2:423694/1‑143 (MQ=255)                 tCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcg                                                                                                                          <  1:672545/143‑1 (MQ=255)                 tCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGGTATGAGCTggcg                                                                                                                          >  1:523009/1‑143 (MQ=255)                     aCGGCGCTTACTGCTGAATTAACTGTCAGCGAAGGTAAGTGGATGACTCATGATGAACCCTGCTCTATGGCTCCAGATGACAAACATGATCTCATATCACGGACTTGTTCGCACCTTCAGGAGGCGTTATGAGCTggaagacc                                                                                                                      >  2:119537/1‑137 (MQ=14)                         cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                                  <  1:204621/143‑1 (MQ=255)                              aCTGCTCAATTCACGGTCGGCGAAGGTAAGTGGATGACTCATGATGATCCCTGTTCTATGGCTCCAGATGACAAGCATGATCTCATATCAGGGACTTGCTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTAt                                                                                                             <  2:704873/143‑1 (MQ=18)                                  ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGa                                                                                                         >  1:348106/1‑143 (MQ=255)                                  ctgAATTCACTGTCGGCGAAGGTAAGTTGATGAACAGAGACCAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTGAGGCGCGGCCGGCCCTTTTTGTATCTgt                                                                                                         >  2:105901/1‑142 (MQ=14)                                    gAAGTCAGTGTCGGCGAAGGTAAGCTGATGACTGAGGGTGAACCCTGGTCTAGGGGTCCAGATGACAAGCATGGTCTCATAGCAGGGACTGGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGatt                                                                                                       <  1:311203/143‑1 (MQ=255)                                        tCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttatta                                                                                                   >  1:102594/1‑143 (MQ=255)                                          aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt                                                                                                 <  2:763233/143‑1 (MQ=255)                                             gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATccc                                                                                              <  1:31769/143‑1 (MQ=255)                                                ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCACCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCAGAGGCGCAATGAGCTGGCGGCGCTGTTTGTATCTGATGATTAATGCCCAc                                                                                           >  2:868361/1‑143 (MQ=17)                                                  cGAAGGGAGGTGGGTGCCTCATGAAGAACCCCGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc                                                                                         <  1:434296/143‑1 (MQ=21)                                                  cGAAGGAAAGGTGAGGACCCAAGAGGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc                                                                                         <  1:437291/143‑1 (MQ=21)                                                        tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAtt                                                                                   <  1:510531/143‑1 (MQ=255)                                                         aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTa                                                                                  <  1:295442/143‑1 (MQ=255)                                                          ctttGATGCGTCATGATTATCCCTGTTCTCTGCCTCGAATTGACAAACATGATCTCATATCCGGGAGTTGTTCGCTCCTTCCGGATGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTaa                                                                                 <  2:6777/141‑1 (MQ=25)                                                           gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt                                                                                                 >  1:4557/1‑126 (MQ=255)                                                           gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt                                                                                                 <  2:4557/126‑1 (MQ=255)                                                               atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGAGATCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTTGGGGTCCTGTTTGTATCTGATTATTAATCACACCCCGCTATTCAGCGcc                                                                            >  2:810354/1‑143 (MQ=18)                                                                tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGccc                                                                           >  1:730865/1‑143 (MQ=255)                                                                   cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTCGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGgc                                                                        >  2:723832/1‑143 (MQ=255)                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGCGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTCATCCACACCACCAATCACGCGCCCAgcc                                                                       >  2:749113/1‑142 (MQ=18)                                                                    tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGgcg                                                                       >  1:125319/1‑143 (MQ=255)                                                                         atgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGGTATGAGCTGGCGGCCCTTTTTGTATCTGATTCGTAATCCCCACCCGCTATTCAGCGCCCGGCGCGGGc                                                                  >  1:218270/1‑143 (MQ=255)                                                                               ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCCGGGACTTGTTCGCACCTTCCGGAGGCGTTCTGAGCTGGCGGCCCTTTTTGTATCTCATTATCCATCCCCACCCGCACTTAAGCCACCCGCGCGGCCATCCGc                                                            >  2:373716/1‑143 (MQ=21)                                                                                     cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGggcgtcggcgtcatt                                                      >  1:73712/1‑140 (MQ=255)                                                                                                     cAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGGTTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGAGTTGACTTTg                                      >  1:483812/1‑143 (MQ=255)                                                                                                      aaaGATGATCTGATATCAGGGACTTGTTCGCACCGTCTGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAGCCCCACCCGCGATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGc                                     <  2:395842/143‑1 (MQ=255)                                                                                                           tGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCt                                <  1:555344/143‑1 (MQ=255)                                                                                                              tctgATATCAGGGACTTGGTCGTTCCTTGCGGAGGCGTTGTGAGCTTGCGGTCCTGTTTGTATCTGATTATTAATCCCCCACCGTTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGtt                             <  2:292793/143‑1 (MQ=255)                                                                                                                tcATGTCAGGGACTTGGTCGCACCTTCCGGAGGCGTTATGCGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTaa                           <  2:782484/143‑1 (MQ=255)                                                                                                                tcAGATCAGGGTCTTGTGCGCACCTCCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTaa                           <  1:825591/143‑1 (MQ=255)                                                                                                                        gggACTTGTTCGCACCTTCCGGAGTCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAAcgcg                   <  2:674500/143‑1 (MQ=255)                                                                                                                        gggACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAAcgcg                   >  1:684744/1‑143 (MQ=255)                                                                                                                          gACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGACCGGCGCGGGCATCTGCGTCTGGTGCATTGTTGAGTTTGCATTCTGTTAAAAAAcaaggt                 >  1:119010/1‑138 (MQ=255)                                                                                                                                 tCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACACACCCGGTATAACaa          >  1:335013/1‑143 (MQ=255)                                                                                                                                  cGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACaaa         <  2:125319/143‑1 (MQ=255)                                                                                                                                         tCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACcttctt  <  1:569961/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTT  >  NC_000913/275011‑275289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.