Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,755,755 IS5 (+) +4 bp coding (58‑61/1413 nt) pykF → pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)24 (1.200) 20/284 0.1 95.9% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 1755755 = 1 (0.050)coding (58/1413 nt) pykF pyruvate kinase I
* ? NC_000913 = 17557581 (0.050)19 (0.950) 18/284 0.2 94.9% coding (61/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region

CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755637‑1755758
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  NC_000913/2102943‑2102802
                                                                                                                                                                                                                                                                        
CGGCAGTGCTCCCAGAAAGCAAGTATCACCTGTGCTTTTCGACTTAAGTACGAGGACTGTCATGAAAAAGTCCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCT                                                                                                                           <  2:265265/143‑1
      ctccCCCAGAAAGCAATTTCCTCCCTTCCTTCTCAACTTAAAGACTAATACTGTGATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATG                                                                                                                     <  2:326605/139‑1
      TGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTACTGAAACAGACCAAAATTGTTCGCACCATCGGACCGAAAACCAAATCTGAGGAGATGTCAGGGAACGAGCGAACAACTCCCTGATAac                                                                                                                     >  2:281035/1‑141
         gacctGAAAGCAAGTTCTTCCGCTCCTTCGCTACTTACAGACTCAGACTGTCATGAAAAAGACCAAAATTGTTGGGACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGA                                                                                                                  <  2:171885/138‑1
              GAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATG                                                                                                             <  1:358963/143‑1
                AAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACCAAGACTGTCATGAAAAAGACCTAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGAGCATGTT                                                                                                           <  2:144620/143‑1
                 AGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCAGAAGAGATGTTAGAGAAAGAACGAACAAGTCCCTGATATGAGATCATGTTT                                                                                                          >  1:41040/1‑143
                      GTTTCTCCCATCCTTCTCAACTTAAATATTTAGTCTGTCATTAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCAT                                                                                                     <  1:209448/143‑1
                        TTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCT                                                                                                   <  2:198767/143‑1
                             CCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCACAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCACTGATATGAGATCATGTTTGTCATCTGGAGC                                                                                              >  1:290838/1‑143
                                 CCTTCTAATATTAAAGTTTAAGATTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATA                                                                                          <  1:163801/143‑1
                                        AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGG                                                                                   >  2:340393/1‑143
                                        AACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGAAGACATAAAACAGG                                                                                   >  1:106955/1‑143
                                              AAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT                                                                             <  1:279076/143‑1
                                               AGAATAAGACTGTCATGAAAAAGACCAAAATTTTTTGCACCATAGGAAAGAAAAACGAAGCGGAAGAGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATC                                                                            <  1:112358/143‑1
                                                 ACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTCGGGAAGGTGCGAACAAGTCCCTGATATGAGCTCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAACAT                                                                          >  2:176392/1‑143
                                                                                           GGACCGAACACCGAGTCTGAGGAGAGGTTGCGCAGGGTGCGAACGAGTCCCTGATTGGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTATCTTCGCCGACAGTGAATTCAGCAGTAA                                <  2:92083/143‑1
                                                                                                                 AGATGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATAAATCATCAACTTACCTTCGCCGACAGAGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAG          >  1:247604/1‑143
                                                                                                                         GGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCAGCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  1:258305/143‑1
                                                                                                                                                                                                                                                                        
CGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1755637‑1755758
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  <  NC_000913/2102943‑2102802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.