Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	46 (1.130)	30/284	0.4	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	55 (1.360)	37/284	0.1	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  >  NC_000913/2101609‑2101885                                                                                                                                             |                                                                                                                                         ttCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTCTTaagga                                                                                                                                        >  2:244128/1‑143 (MQ=255)  tCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaa                                                                                                                                       <  2:380351/143‑1 (MQ=255)        tACATAAAGTAACCTTGATCGAAGCCATTTACACGCTAACAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCg                                                                                                                                 <  1:496627/143‑1 (MQ=255)             aaaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTCTTATTAAGGAAGGTGCGAAtta                                                                                                                            >  2:615858/1‑141 (MQ=255)                 tAACCTTGGTCGAAGCCATTTACACGCACAAAAGCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  2:419240/143‑1 (MQ=255)                 tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  2:237469/143‑1 (MQ=255)                 tAACCGGGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTGCCTGGGTGAATCACCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCgg                                                                                                                        <  1:726453/143‑1 (MQ=255)                      ttGATCGAAGCCATTTACCCGCCCAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACATCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                   <  2:427939/143‑1 (MQ=255)                        gATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAGTTTTTGTTTTGTTAAGGAAGGTGCGAATAAGCGGGGaggtt                                                                                                                 >  1:69363/1‑139 (MQ=255)                          tCGAAGCCATTTACACGCACAAAATCTGAACAAAGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAttct                                                                                                               <  1:568782/143‑1 (MQ=255)                           cGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCATCATAAATGATCCTGCGCACTAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGGATTTTTGTTGTGTTAAAGAAGGTGCGAATAATCGGGCAAAtgctt                                                                                                              >  1:151852/1‑143 (MQ=255)                                gcATTCACACGAACAAAATCTAAAAAACCTACCAGCATAAATGATCCTTCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                         <  2:660360/142‑1 (MQ=39)                                ccATTTACACGCACAAGATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                         <  1:36774/143‑1 (MQ=255)                                ccATTTACACGCACAAAATCTGAAACACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAAAATACACACTCTCTTGAGGAATTTTTGTTTTCTTAAGGTAGGGGCGAATAAGCGGGACAATTctcctctg                                                                                                         >  2:186914/1‑141 (MQ=255)                                  aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTTCGCACTAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                       <  1:335820/143‑1 (MQ=255)                                  aTTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                       >  1:288390/1‑143 (MQ=255)                                             caAAATCTGAAAAACGTACCAGCATAAAAGAGCCGGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                            <  1:304171/143‑1 (MQ=255)                                                 aTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttca                                                                                        <  1:477995/143‑1 (MQ=255)                                                  tCTGACAAACGGACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTGAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                       <  2:98357/143‑1 (MQ=255)                                                  tCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGCATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                       >  1:385035/1‑143 (MQ=255)                                                     gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGAGAAATTCTTCTCGGATGAACACTTCatttcatttc                                                                                    >  2:64099/1‑143 (MQ=38)                                                       aaaaCGTAGCAGCAGAAATCATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTATGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                  <  2:10608/143‑1 (MQ=255)                                                        aaaCGTAGCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                 <  2:56916/143‑1 (MQ=255)                                                          aCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAt                                                                               <  2:257264/143‑1 (MQ=255)                                                              aCCAGCATAAATGATCCTGCGCACCAAGCAACAACCGTATCAGAATAGATACTCACACTACGAATTTATAAATCATTAAGGAAGGTGCGAATAAGCGTGGCATTTCCTAAGGGCTGACCCAGTCATTTCATTTCTTCATGttt                                                                           <  2:295886/143‑1 (MQ=16)                                                                cAGTATAAATGATCTTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                         <  1:481853/143‑1 (MQ=255)                                                                cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTTCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                         <  2:528954/143‑1 (MQ=255)                                                                     tAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa                                                                    <  2:360724/143‑1 (MQ=255)                                                                      aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                   >  1:345866/1‑143 (MQ=255)                                                                      aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGAGACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                   <  2:612271/143‑1 (MQ=255)                                                                            tCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                             >  1:478359/1‑143 (MQ=255)                                                                                gcgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTccc                                                         <  1:58977/143‑1 (MQ=255)                                                                                       aaTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTCAGCCGCTCTTTTCTCCCTTCAATg                                                  >  2:538430/1‑143 (MQ=21)                                                                                        aTCAACAACCGTATCAGAATAGATACTTTCTTAAGGACTTTTTGTTTTATTAAGGAAGGTGCGAATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTCTTTCTCGCGTACATGc                                                 >  1:545471/1‑143 (MQ=21)                                                                                                   tATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATGTTTTCTCCCGTAAATGCGTTGAATCatc                                      >  2:511342/1‑141 (MQ=255)                                                                                                     tCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGAGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTATATGCCTTTAATCAGCCt                                    >  2:365648/1‑143 (MQ=255)                                                                                                        gAATATCTACCTTCTTTAGGAATTTGTGTCTTATTCATGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                                 <  2:503676/143‑1 (MQ=14)                                                                                                               tACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                          >  1:569385/1‑143 (MQ=255)                                                                                                                      tttAGGAATTTTTGTTTTATTAAGGAAGGTGCGTATATGCTGGGAAATTCTTCTCTGCTTACTCAGTCATTTCATTTCTTCATGTTTGAGCTGATTTTTTCTCCCGTAAATGCCTTGAATCAACCTATTTAGACCGTttcttc                   <  2:99604/143‑1 (MQ=14)                                                                                                                      tttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGGCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAACGTtttttc                   <  2:683131/143‑1 (MQ=255)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                             <  2:371753/98‑1 (MQ=255)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATATTTGAGCCGATTTTTtc                                                             >  1:371753/1‑98 (MQ=255)                                                                                                                           gAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCACTGCCTTGACTCAGCCTATCTAGCCCGTTTCTCCGCCac              >  2:111158/1‑142 (MQ=14)                                                                                                                                  tGTTTTATTAATGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGTTGTCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc       <  2:478359/143‑1 (MQ=255)                                                                                                                                    ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGTGCCGATTTTTTCTCCCTTATATGCCTTGGATCTGCCTATTTAGACCGGTCCTTGCCCCTTTTAGGCgg     >  2:538327/1‑142 (MQ=11)                                                                                                                                     tttATCAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt    <  1:450560/143‑1 (MQ=17)                                                                                                                                       tattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTACGGCGTTAt  >  2:407142/1‑143 (MQ=17)                                                                                                                                             |                                                                                                                                         TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAT  >  NC_000913/2101609‑2101885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.