Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	46 (1.130)	30/284	0.4	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	55 (1.360)	37/284	0.1	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255                                                                                                                                              |                                                                                                                                               aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGGACCCTGTTCTATGGCTCCAGATGACAAACGTGAGCTCATATCAGGGACTTGTTCGCGCCTTCCa                                                                                                                                               >  2:590037/1‑143 (MQ=2)    aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATGCACTGTCGGCGAAGGTAAGTTAATGACTCATGATAAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCGGGGACTTGTTCGCACATTCCATag                                                                                                                                            >  1:700125/1‑142 (MQ=11)     aaaTCTCTTTTCGGGTCTGACGGCGGTTAAGGCTGAATTCACCGTCGGCGAAGGTAAGTGGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAc                                                                                                                                           <  1:445026/143‑1 (MQ=11)          tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                      >  1:595038/1‑143 (MQ=37)          tctTTTCTGGTCTGACGGCGCGTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                      <  1:70277/143‑1 (MQ=37)                 tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCAACTGCCATAACGCTGTCGccacc                                                                                                                               >  2:209289/1‑143 (MQ=255)                       gACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACa                                                                                                                         >  1:16337/1‑143 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                <  1:658396/135‑1 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccac                                                                                                                                >  2:658396/1‑135 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAg                                                                                                                        >  1:29214/1‑143 (MQ=255)                               ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                                 <  1:591520/143‑1 (MQ=255)                                   cgttgAATTGACTGTCGGCGAAGGGAAGGTGATCACTCATGATGAACCCTGTTCTATGTCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCTCCAGAACAGATATTGCGGaa                                                                                                             <  2:614893/140‑1 (MQ=17)                                    gctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAAc                                                                                                            <  2:392307/143‑1 (MQ=255)                                     ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCATCCATAACGCTGTCGCAACCCGAACAGAAGTTGcaccacg                                                                                                           >  2:145606/1‑137 (MQ=14)                                     ctgAATTCACTGTCGGCGAAGGTAAGTTGAAGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTAGAGCCACCAGAACCGACATAGTGAAACg                                                                                                           >  2:431624/1‑143 (MQ=14)                                          ttCACTGTCGGCGAATGTACTCTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGCGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGTAACGACaaa                                                                                                      <  1:108798/143‑1 (MQ=18)                                              cTGGCGGCGAACGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAgaga                                                                                                  <  2:89531/143‑1 (MQ=255)                                                gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTCCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGaaa                                                                                                <  1:239719/143‑1 (MQ=255)                                                   ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAg                                                                                             <  1:139152/143‑1 (MQ=255)                                                    gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGGCAAAGAGAAACAGa                                                                                            >  1:238235/1‑143 (MQ=255)                                                               ttGATGACTCATGATGAACCATGTTCTATGGCACCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACACTGTAGCAACCAAAAAAGATATTGAGTAACGAAAAAGAGAAACAAAACCaaaataaa                                                                                 >  2:382822/1‑136 (MQ=17)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAACGCTGTAGCCACCAGACCAGATATTGCGGAACGACAACGAGAAACAGAACCAGATTGATGCATTGAg                                                                         >  1:661999/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCCGAACAGCAATTGCGGAACGACCAAGACAAACAGAAACAGATTGATGCATTGAg                                                                         >  2:181567/1‑143 (MQ=21)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                         >  1:347446/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAg                                                                         >  1:531652/1‑143 (MQ=255)                                                                       tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATATCATATCAGGGACTTGGTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGCGACGACAAAGAGAAACAGAACCAGATTGATGCATGGAg                                                                         >  1:692020/1‑143 (MQ=25)                                                                         atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGGAACAGAACCAGCTTGATGCATTGCGCt                                                                       >  1:409543/1‑143 (MQ=255)                                                                               agcccTGTCCGATGCCTCCAGATGACACACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGGACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATc                                                                 <  1:597536/141‑1 (MQ=37)                                                                                        cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTGTGAaat                                                        >  1:470257/1‑143 (MQ=255)                                                                                         tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaatt                                                       >  1:328587/1‑143 (MQ=255)                                                                                            gccTCCATATGACAAACATGATCTCATATCAGGGATTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATAGTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaat                                                    <  1:83565/141‑1 (MQ=255)                                                                                             gCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaatt                                                   <  1:391043/143‑1 (MQ=255)                                                                                                 cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAAAACGAGAGGGATGCATTGAGCTTTCATCCTAAGAAATTAATTGCTg                                               >  2:547993/1‑143 (MQ=38)                                                                                                  aGATGACCACCATGACCTCATATCAGCGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGt                                              <  2:173395/143‑1 (MQ=255)                                                                                                   gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGtt                                             <  1:381397/143‑1 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  1:2286/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  1:572940/1‑143 (MQ=255)                                                                                                         aaaCATGATCTCATATCAGGGACTTGTTCGCACCTGCCATAACGCTGTAGCCACCAGAACAGATATTGAGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGCGCTTTCATCCTATGAAATTAATTCCTGTTAAACGc                                       >  2:499803/1‑143 (MQ=39)                                                                                                         aaaCATGATCTCATATCAGAGACGGGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  2:91474/1‑143 (MQ=255)                                                                                                           aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAt                                     >  1:102338/1‑143 (MQ=255)                                                                                                                aTCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGt                                >  2:727925/1‑143 (MQ=255)                                                                                                                   tcATATCAGGGACTTGCTCGCACCCTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACa                             <  1:86838/143‑1 (MQ=255)                                                                                                                          aGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATa                      <  1:548039/143‑1 (MQ=255)                                                                                                                           gggACTTGTTAGCACCAACCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc                     <  1:194385/143‑1 (MQ=255)                                                                                                                            ggACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                    >  2:423410/1‑143 (MQ=255)                                                                                                                                       cACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACCAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAACTAATTGCTGTTAAAACCATTGGGTACAGAAAACACCCATACCTccct         >  2:558593/1‑141 (MQ=255)                                                                                                                                              cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  >  2:662068/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                               AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289972‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.