mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | pgi-13-3_S27_L001_R1_001.good.fq | 1,416,044 | 384,500,930 | 100.0% | 271.5 bases | 289 bases | 99.7% |
| errors | pgi-13-3_S27_L001_R2_001.good.fq | 1,416,044 | 385,259,625 | 100.0% | 272.1 bases | 289 bases | 94.1% |
| total | 2,832,088 | 769,760,555 | 100.0% | 271.8 bases | 289 bases | 96.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,653 | 161.4 | 5.3 | 100.0% | Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group |
| total | 4,641,653 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 7300 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 440 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.055 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.82355 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | OFF |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.025 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.8 |
| R | 3.3.1 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 02:34:16 19 Mar 2018 | 02:34:59 19 Mar 2018 | 43 seconds |
| Read alignment to reference genome | 02:35:00 19 Mar 2018 | 02:39:20 19 Mar 2018 | 4 minutes 20 seconds |
| Preprocessing alignments for candidate junction identification | 02:39:20 19 Mar 2018 | 02:40:15 19 Mar 2018 | 55 seconds |
| Preliminary analysis of coverage distribution | 02:40:15 19 Mar 2018 | 02:42:14 19 Mar 2018 | 1 minute 59 seconds |
| Identifying junction candidates | 02:42:14 19 Mar 2018 | 02:42:23 19 Mar 2018 | 9 seconds |
| Re-alignment to junction candidates | 02:42:23 19 Mar 2018 | 02:43:08 19 Mar 2018 | 45 seconds |
| Resolving best read alignments | 02:43:08 19 Mar 2018 | 02:44:38 19 Mar 2018 | 1 minute 30 seconds |
| Creating BAM files | 02:44:38 19 Mar 2018 | 02:46:12 19 Mar 2018 | 1 minute 34 seconds |
| Tabulating error counts | 02:46:12 19 Mar 2018 | 02:47:16 19 Mar 2018 | 1 minute 4 seconds |
| Re-calibrating base error rates | 02:47:16 19 Mar 2018 | 02:47:17 19 Mar 2018 | 1 second |
| Examining read alignment evidence | 02:47:17 19 Mar 2018 | 04:28:39 19 Mar 2018 | 1 hour 41 minutes 22 seconds |
| Polymorphism statistics | 04:28:39 19 Mar 2018 | 04:28:40 19 Mar 2018 | 1 second |
| Output | 04:28:40 19 Mar 2018 | 04:29:07 19 Mar 2018 | 27 seconds |
| Total | 1 hour 54 minutes 50 seconds | ||
