Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503	1979278	776	40 [2]	[0] 38	insB1–insA	insB1, insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	2 (0.070)	38 (1.440)	36/268	0.0	97.6%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGG  >  NC_000913/1978365‑1978635                                                                                                                                          |                                                                                                                                      cTTTACACTTATCTAAGATTTTTCCTAAATCGACGAAACTGTACTCGTCACTACACGCACATACAACGGAGGGGTGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                  <  1:158694‑M1/143‑6 (MQ=255)   gtaCACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                                                                <  2:108651‑M1/142‑8 (MQ=255)             cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                                                      >  2:220912‑M1/1‑126 (MQ=255)                  gtttttcCTAGATCGAGGCGTCTGTCCTCGTCTCTACACGCACATACAGCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAATACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                 <  2:402083‑M1/142‑23 (MQ=255)                   tttttCCTAAATCGACGCAACTGTACTCGTCACTGCACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAACACACTCAATTACTTAACATAACTGtataactcatacttttgttttgg                                                                                                                >  2:31863‑M1/1‑120 (MQ=255)                   tttttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                <  1:319096‑M1/143‑24 (MQ=255)                   tttttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                <  1:323229‑M1/143‑24 (MQ=255)                           aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattt                                                                                                        >  1:267528‑M1/1‑112 (MQ=255)                             aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACCTAAATGtataaatcatacttttgtcttgggtgtatttca                                                                                                      >  2:469116‑M1/1‑110 (MQ=255)                                   gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                <  1:440322‑M1/143‑40 (MQ=255)                                   gCAACTGTACTCGTCACTACACGCACATAAAAAGGAGGGGGGCTGCGATTTTCAATAATGCGAGATGCAGATCACACAAAACACTCAATTACTTAAAATAAATGtataagacagactattgatttaggtgtagtttaatatgt                                                                                                >  2:294187‑M1/1‑104 (MQ=255)                                          tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                                                         <  1:249079‑M1/143‑47 (MQ=255)                                           aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATACGTAATGCAGAACACAAAAAACACTCAATTACTTAAAATAAATGtataagtcataattttgtatgtggtgtatttcaatatgtaaaaaaga                                                                                        >  2:128379‑M1/1‑96 (MQ=255)                                             tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttagttttgggtgtatttcaatctgttaaaaagttt                                                                                      >  1:328904‑M1/1‑94 (MQ=255)                                               gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaagcgggtaatacgttttt                                                                                    >  1:86531‑M1/1‑92 (MQ=255)                                                  aCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagattttcgc                                                                                 >  2:324428‑M1/1‑89 (MQ=255)                                                       acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtgtttcaatctgttaaaaagtttttcgctacgc                                                                            >  1:94382‑M1/1‑84 (MQ=255)                                                         gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacatttgttttggagcgacgtcaatcagttaaaaagtttttcgctacgcta                                                                          >  2:212789‑M1/1‑82 (MQ=255)                                                         gCACAGACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                          <  1:73071‑M1/143‑62 (MQ=255)                                                           acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagc                                                                        <  1:322975‑M1/143‑64 (MQ=255)                                                           acaTACAACGGAGGGGGGCTACGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttatgttttgggtgtatttcaatctgttaacaactttttcgctacgctaca                                                                        >  2:41157‑M1/1‑80 (MQ=255)                                                                         ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTGAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                          >  1:463479‑M1/1‑66 (MQ=255)                                                                         ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcagacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                          <  1:379163‑M1/143‑78 (MQ=255)                                                                                        aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa                                           >  2:231327‑M1/1‑51 (MQ=255)                                                                                              gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaaaggaataatcgacatgggaa                                     >  2:424073‑M1/1‑45 (MQ=255)                                                                                                gTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataataaaaatgagagtg                                   >  1:63358‑M1/1‑43 (MQ=255)                                                                                                  gATGGAGAGCACACAAAAATATCAATGATTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttagaaagtttttcgctacgatagcaagcaaaaatgaaacaggaataatcgaaatgggatgttg                                 <  2:463479‑M1/143‑103 (MQ=255)                                                                                                   aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                <  1:266004‑M1/143‑104 (MQ=255)                                                                                                            acacaAAACACTCAATTACTTAACATAAATGtataagtcagacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtca                       <  1:469333‑M1/143‑113 (MQ=255)                                                                                                            acaaaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtacttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtca                       <  2:239276‑M1/143‑113 (MQ=255)                                                                                                               caAAACACGCAATTACTTAACATAAATGtagaagtcatactgttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                    <  1:57132‑M1/143‑116 (MQ=255)                                                                                                                  accactcacttaCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaagcgggtaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                 <  1:129575‑M1/141‑119 (MQ=255)                                                                                                                  aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcacaaataaaacaggaataatcgaaatgagatgttgcgcacagtcaacataa                 >  2:261833‑M1/1‑25 (MQ=255)                                                                                                                       tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataacacac            >  2:83360‑M1/1‑20 (MQ=255)                                                                                                                          aTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaacagtttttcgctacgctagcaagaaaaaacgaaacaaaaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgt         >  2:290541‑M1/1‑17 (MQ=255)                                                                                                                               ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtctagataactcaccgtaaata    >  1:8381‑M1/1‑12 (MQ=255)                                                                                                                               ttAACAGAAATGtagaaggcatactgttgtggtgggtgtagttcaagcggttaaagagttttgcgctacgcgagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata    <  2:117936‑M1/143‑132 (MQ=255)                                                                                                                                tcgtggtggcgggTACTGGCTCCAACTGATTCATAGTGTTTTATGGTCAGATAATGCCCGATGACCTTGTCAGGCAGCTCCACCGAGTTGGAGACCGGCGGCCACTCCCCTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAg   <  2:216564/133‑1 (MQ=2)                                                                                                                                attttgtgccgggTAATGACTCCCACTTATTGATCGTGTTTTATGTTCAGATAATGCCCCATGCATTTCTCATGCAGCTAAAACGGCGGGGGGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAg   <  1:112556/133‑1 (MQ=2)                                                                                                                                 aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataat  >  2:93816‑M1/1‑10 (MQ=255)                                                                                                                                          |                                                                                                                                      CTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGG  >  NC_000913/1978365‑1978635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑