Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299499–1300697 1300697 1–1199 36 [0] [0] 36 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)35 (0.890) 32/276 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299363‑1299638
                                                                                                                                       |                                                                                                                                            
agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGGTACAGGAAAGTGCTAAataataa                                                                                                                                       >  1:93380‑M1/1‑136 (MQ=255)
           aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                            <  1:481736‑M1/143‑19 (MQ=255)
           aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                            <  2:515668‑M1/143‑19 (MQ=255)
            ggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAAGTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                           <  2:114059‑M1/143‑20 (MQ=255)
                ttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                       >  2:398291‑M1/1‑120 (MQ=255)
                 tCCTTAACTGCTTCTCTTCATCATCATTTTATTTTCTTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                      <  2:234568‑M1/143‑25 (MQ=255)
                   cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcacttgttaaattattgt                                                                                                                    <  1:615780‑M1/143‑27 (MQ=255)
                    ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGCTAGAGGAAAGTGCTAAataataatcaattgttaaattaatgtg                                                                                                                   >  1:126913‑M1/1‑116 (MQ=255)
                      aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgca                                                                                                                 >  1:526306‑M1/1‑114 (MQ=255)
                         tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCGCTGCCTTTGGTAGAGAAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                              >  2:551518‑M1/1‑111 (MQ=255)
                                 ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                      >  2:148155‑M1/1‑103 (MQ=255)
                                  tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                     >  1:86582‑M1/1‑102 (MQ=255)
                                      catgatTTTATTTTCGTCACATTATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                 <  1:90363‑M1/143‑46 (MQ=255)
                                        tcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                               <  2:592481‑M1/143‑48 (MQ=255)
                                           tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                            >  1:131927‑M1/1‑93 (MQ=255)
                                            gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                           <  2:63403‑M1/143‑52 (MQ=255)
                                                ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAActaataatccattgttctattatggtgcatttcaattctgtaactgtaatcagaa                                                                                       >  2:59970‑M1/1‑88 (MQ=255)
                                                  ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                     <  2:91790‑M1/143‑58 (MQ=255)
                                                     cccaCATCATAATCCTGGGCTTGCTTAAGAATAATTTAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                  <  2:438021‑M1/142‑61 (MQ=255)
                                                      ccACGTGATAATCCTTGCTTTGGTGAAGAATAATTGCAATGGTATTATTAATTCCACTGTCTTTTGTAGAGGAAAGTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                 <  1:587260‑M1/143‑62 (MQ=255)
                                                        acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACAGCCAGTGGGAGAGCCAACTGCTAAataataatcaaccgttaaatcattgtccatttcactactggaactgcaatcagaaacgataga                                                                               >  1:118730‑M1/1‑80 (MQ=255)
                                                            catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgcaactgtaatcagaaaagatagacaag                                                                           >  2:372318‑M1/1‑76 (MQ=255)
                                                                         ttGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaagatagacatgcttagccaatcac                                                              >  1:346272‑M1/1‑63 (MQ=255)
                                                                          tGCTGAAGAATAATTGAATTGATATTATTAATTACACTGCCTTTGGTAGAGGAAAGAGCTAAaaaataatcaattgttaaattatggtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                             <  2:120699‑M1/143‑82 (MQ=255)
                                                                                 gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgat                                                      <  1:24151‑M1/143‑89 (MQ=255)
                                                                                    taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatgagaaaagatagacatgcttagccaatctctattagattga                                                   >  2:57072‑M1/1‑52 (MQ=255)
                                                                                                  ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                     <  2:131927‑M1/143‑106 (MQ=255)
                                                                                                  ttagtaATTCCAAAGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                     <  1:667547‑M1/143‑106 (MQ=255)
                                                                                                     ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagagaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                  >  2:609181‑M1/1‑35 (MQ=255)
                                                                                                      tcattCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtta                                 <  1:531635‑M1/141‑110 (MQ=255)
                                                                                                              ttgtctttgTTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                         <  2:46608‑M1/139‑118 (MQ=255)
                                                                                                                        tAGATGAAAGTGCTAAatattaatcatttgttaaattattgtgcatttcactattggaactgtaattagaaaagatagacatgcttagtcaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               <  1:21352‑M1/143‑128 (MQ=255)
                                                                                                                        tAGAGGAAAGCGGTAAataataatcaactgttaaattattgttcatttcactaatggaactgaaaccagaaaagatacaaatgtttatccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               <  2:332871‑M1/143‑128 (MQ=255)
                                                                                                                         agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat              >  2:210289‑M1/1‑15 (MQ=255)
                                                                                                                                   gCTAAataattatcaattgtaaaattattgtgcttttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat    <  2:93380‑M1/143‑139 (MQ=255)
                                                                                                                                     tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggctgcaagctatagattctgatac  >  1:684565‑M1/1‑3 (MQ=255)
                                                                                                                                       |                                                                                                                                            
AGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299363‑1299638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.