Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299499–1300697 1300697 1–1199 36 [0] [0] 36 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)35 (0.890) 32/276 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300562‑1300837
                                                                                                                                        |                                                                                                                                           
agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactccctttggtacaggaaagtgctaaataataa                                                                                                                                       >  1:93380‑M2/137‑143 (MQ=255)
           aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctggagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                            <  1:481736‑M2/18‑1 (MQ=255)
           aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                            <  2:515668‑M2/18‑1 (MQ=255)
            ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataagtgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                           <  2:114059‑M2/19‑1 (MQ=255)
                ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                       >  2:398291‑M2/121‑143 (MQ=255)
                 tccttaactgcttctcttcatcatcattttattttcttcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                      <  2:234568‑M2/24‑1 (MQ=255)
                   cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAATTATtgt                                                                                                                    <  1:615780‑M2/26‑1 (MQ=255)
                    ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagactaattgaaatgatattattaattccactgcctttgctagaggaaagtgctaaATAATAATCAATTGTTAAATTAAtgtg                                                                                                                   >  1:126913‑M2/117‑143 (MQ=255)
                      aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                 >  1:526306‑M2/115‑143 (MQ=255)
                         tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccgctgcctttggtagagaaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                              >  2:551518‑M2/112‑143 (MQ=255)
                                 ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                      >  2:148155‑M2/104‑143 (MQ=255)
                                  tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                     >  1:86582‑M2/103‑143 (MQ=255)
                                      catgattttattttcgtcacattataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                 <  1:90363‑M2/45‑1 (MQ=255)
                                        tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                               <  2:592481‑M2/47‑1 (MQ=255)
                                           tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                            >  1:131927‑M2/94‑143 (MQ=255)
                                            gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                           <  2:63403‑M2/51‑1 (MQ=255)
                                                ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaCTAATAATCCATTGTTCTATTATGGTGCATTTCAATTCTGTAACTGTAATCAGaa                                                                                       >  2:59970‑M2/89‑143 (MQ=255)
                                                  ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                     <  2:91790‑M2/57‑1 (MQ=255)
                                                     cccacatcataatcctgggcttgcttaagaataatttaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                  <  2:438021‑M2/60‑1 (MQ=255)
                                                      ccacgtgataatccttgctttggtgaagaataattgcaatggtattattaattccactgtcttttgtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                 <  1:587260‑M2/61‑1 (MQ=255)
                                                        acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacagccagtgggagagccaactgctaaATAATAATCAACCGTTAAATCATTGTCCATTTCACTACTGGAACTGCAATCAGAAACGATAGa                                                                               >  1:118730‑M2/81‑143 (MQ=255)
                                                            cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGCAACTGTAATCAGAAAAGATAGacaag                                                                           >  2:372318‑M2/77‑141 (MQ=255)
                                                                         ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAAGATAGACATGCTTAGCCaatcac                                                              >  1:346272‑M2/64‑141 (MQ=255)
                                                                          tgctgaagaataattgaattgatattattaattacactgcctttggtagaggaaagagctaaAAAATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                             <  2:120699‑M2/81‑1 (MQ=255)
                                                                                 gaataattgaaatgatattattaattccactgccttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                      <  1:24151‑M2/88‑1 (MQ=255)
                                                                                    taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATGAGAAAAGATAGACATGCTTAGCCAATCTCTATtagattga                                                   >  2:57072‑M2/53‑143 (MQ=255)
                                                                                                  ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                     <  2:131927‑M2/105‑1 (MQ=255)
                                                                                                  ttagtaattccaaagcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                     <  1:667547‑M2/105‑1 (MQ=255)
                                                                                                     ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAGAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                  >  2:609181‑M2/36‑143 (MQ=255)
                                                                                                      tcattccactgccttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTa                                 <  1:531635‑M2/109‑1 (MQ=255)
                                                                                                              ttgtctttgttagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                         <  2:46608‑M2/117‑1 (MQ=255)
                                                                                                                        tagatgaaagtgctaaATATTAATCATTTGTTAAATTATTGTGCATTTCACTATTGGAACTGTAATTAGAAAAGATAGACATGCTTAGTCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta               <  1:21352‑M2/127‑1 (MQ=255)
                                                                                                                        tagaggaaagcggtaaATAATAATCAACTGTTAAATTATTGTTCATTTCACTAATGGAACTGAAACCAGAAAAGATACAAATGTTTATCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta               <  2:332871‑M2/127‑1 (MQ=255)
                                                                                                                         agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat              >  2:210289‑M2/16‑143 (MQ=255)
                                                                                                                                   gctaaATAATTATCAATTGTAAAATTATTGTGCTTTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt    <  2:93380‑M2/138‑1 (MQ=255)
                                                                                                                                     taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGCTGCAAGCTATAGATTCTGATAc  >  1:684565‑M2/4‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300562‑1300837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.