Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,049,990 Δ12 bp noncoding (93‑104/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40499891 (0.020)47 (1.170) 42/282 0.0 97.9% noncoding (92/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 4050002 = NA (NA)noncoding (105/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

AATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAA  >  NC_000913/4049847‑4050131
                                                                                                                                              |                                                                                                                                              
aaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAT                                                                                                                                                <  2:248368‑M1/143‑1 (MQ=255)
   tacaaatatttttttctgaatttaacaAAAAAGAGTAAAGGTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttt                                                                                                                                             <  2:502070‑M1/131‑4 (MQ=255)
   tACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttt                                                                                                                                             <  1:227316‑M1/143‑4 (MQ=255)
      aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCATTCAGTAATttttct                                                                                                                                          >  1:541952‑M1/1‑137 (MQ=255)
       aaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttatt                                                                                                                                         <  1:359036‑M1/143‑8 (MQ=255)
          aGTGGTTTCTGAAGCGAACAAAAAAGAGGAAAGGGAGTCGCGGAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattggg                                                                                                                                      <  2:150880‑M1/143‑11 (MQ=255)
                  ctgaactgaaCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggagaaagaa                                                                                                                              >  2:467310‑M1/1‑125 (MQ=255)
                  ctgaactgaaCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATatatatagggcgaaaaaa                                                                                                                              >  2:573614‑M1/1‑125 (MQ=255)
                    gaaccaaacaagaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaa                                                                                                                            <  2:226017‑M1/137‑21 (MQ=255)
                       ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagat                                                                                                                         >  1:482517‑M1/1‑120 (MQ=255)
                        tgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcggatgaacagatc                                                                                                                        >  2:673249‑M1/1‑119 (MQ=255)
                         gaacAAAAAAGAGTTAAGTTAGTCGCGTATTTTATAGAGTTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatcc                                                                                                                       <  2:272506‑M1/143‑26 (MQ=255)
                            cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTACTttttattgggcgaaagaaaagatccgta                                                                                                                    >  2:168109‑M1/1‑115 (MQ=255)
                            cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTATAGAAGCAAAAGTCAGTAATttttattgggcgaaagaaaagatccgta                                                                                                                    >  2:190281‑M1/1‑115 (MQ=255)
                                    agTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaaggcctga                                                                                                            <  1:472596‑M1/143‑37 (MQ=255)
                                            tAGTCGCGTAGGGTACAGAGGGAAGATGTTCTATCTTTTAGGGCTTGTATTTCACGTGAGCGGATTTGGCTGGGTATTTTGGCGGCCGCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctat                                                                                                    >  2:705168‑M1/1‑99 (MQ=255)
                                                cgcgTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgttt                                                                                                <  1:460352‑M1/143‑49 (MQ=255)
                                                  cgTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATtttttttgtgcgaaagaaaagatccgtaatgcctgatacgctatgtttat                                                                                              >  2:121868‑M1/1‑93 (MQ=255)
                                                  cgTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATATTAGCCGCCCCAGTCAGTAATttttactgggcgaaagaaaagatccgtaatgcgtgattcgatatgtttat                                                                                              >  1:456488‑M1/1‑93 (MQ=255)
                                                    tAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTATTttttttttgtcttatgaaaagatccataatgcctgatgcgctatatttataa                                                                                            >  2:55177‑M1/1‑91 (MQ=255)
                                                     ttgttacagTGGTTATATGTTCTATCCTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcag                                                                                           <  2:452976‑M1/139‑54 (MQ=255)
                                                            agagGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacg                                                                                    >  2:381979‑M1/1‑83 (MQ=255)
                                                                  aattttttctatctTTCATATCTTGTACTTCACGGAATCGGAATTGGCTGAATAGTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaa                                                                              <  2:563083‑M1/137‑67 (MQ=255)
                                                                             tCTTTCATACTTTTTTCTTGCCGTATGCGGGTGTGGTTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctat                                                                   <  1:722379‑M1/143‑78 (MQ=255)
                                                                             tCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCTAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgttatgtttatcagagcaacggtagaattgtaaacgcg                                                                   >  1:270064‑M1/1‑66 (MQ=255)
                                                                                ttCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgttgcgctatgtttataaggacaacggtagaattgtaatctattga                                                                >  2:86413‑M1/1‑63 (MQ=255)
                                                                                     aCCTTTTCCTTCTCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaattta                                                           <  1:409074‑M1/143‑86 (MQ=255)
                                                                                     aCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccgtcggtcgaattgtaatctattgaattta                                                           >  2:293679‑M1/1‑58 (MQ=255)
                                                                                      ccTGTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttac                                                          <  1:574678‑M1/143‑87 (MQ=255)
                                                                                       cTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTGAGTAAT‑‑‑TG‑‑‑‑‑‑‑‑‑TTATTGGGCGAAAGCAAAGATCCGTAATGCATGAACAAATATATTTATCAGACCAAAGGTAGAATTGTAATATATTGAAATTaaa                                             >  2:651683/1‑141 (MQ=255)
                                                                                       cTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgctgcgctatgtttatgaggccaacggtaaaattgtaatctattgaatttaca                                                         >  2:581850‑M1/1‑56 (MQ=255)
                                                                                             cTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccgg                                                   >  1:559067‑M1/1‑50 (MQ=255)
                                                                                                cACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctttgtttatcaggcctacggtagatttgtcatttatagaaattacgagccggata                                                >  2:240678‑M1/1‑47 (MQ=255)
                                                                                                cACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggata                                                >  1:396935‑M1/1‑47 (MQ=255)
                                                                                                 aCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatac                                               >  2:567099‑M1/1‑46 (MQ=255)
                                                                                                  cGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgctgcgctatgtttatccggccaacggtagaattgtaatctaatgaatttacgggccggatacg                                              >  2:425100‑M1/1‑45 (MQ=255)
                                                                                                   ttaACCGGATTTGTCTGAATATTTTAGCCGTCACAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgc                                             <  2:643214‑M1/142‑100 (MQ=255)
                                                                                                     aaTCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttaccgaccggatacgaca                                           >  1:364664‑M1/1‑42 (MQ=255)
                                                                                                         ggATTTGGCTAAATATGTTAGCCGCCCCATTCAGTAATttttattgtgcgaaagaaaagatccgtaatgcctgatgcgctatgtgtatcatgccaacagtagaattgtaatctattgaatttacgggccggatacgccacatc                                       <  1:329866‑M1/143‑106 (MQ=255)
                                                                                                            tttGGCTGAATATTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccgg                                    <  2:732089‑M1/143‑109 (MQ=255)
                                                                                                                    aaTATTTTAGCCGCCCCAGTCAGTAATttttattggacgaaagaaaagatccgtaatgcctgatgcgctatgtttatcagaccaacggaagagttgtaaactatgaaatttacgggccggatacgcgaccaccggcacaaaga                            >  1:551963‑M1/1‑27 (MQ=255)
                                                                                                                      tatTTTAGCCGCCCCAGTCAGTAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctcttgactttacgggccggctacgccacatccggcacaagcatt                          >  2:148787‑M1/1‑25 (MQ=255)
                                                                                                                         tttAGCCTCCCCAGTCAGTGATttttcttgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcacaagcattaag                       <  1:259779‑M1/143‑122 (MQ=255)
                                                                                                                                 ccccAGTCAGTAATttttattaggcgaaagaaaagatctgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgagccggatacgccacatcaggcacaagcattacgtcaagaaa               >  1:85346‑M1/1‑14 (MQ=255)
                                                                                                                                    cagtcagtAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcaccagccttaaggcaagaacatt            >  2:633051‑M1/1‑11 (MQ=255)
                                                                                                                                        cagtAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcacaagcattaaggcaagaaaattattc        >  1:21116‑M1/1‑7 (MQ=255)
                                                                                                                                         agtAATttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtactctattgaatttacgggccggatacgcaaaatccggcaaaagcattaaggcaagaaaattattaa       >  2:147462‑M1/1‑6 (MQ=255)
                                                                                                                                            aaTttttattgggcgaaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcacaagcattaaggcaagaaaattattcgccg    >  1:534334‑M1/1‑3 (MQ=255)
                                                                                                                                             aTttttattgggcgcaagaaaagatccgtaatgcctgatgcgctatgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcacaaacattaaggcaagaaaattattcgccgt   >  1:458666‑M1/1‑2 (MQ=255)
                                                                                                                                              tttttattggtagatagaaaagatgcgtaatgccggatgcgctgtgtttatcaggccaacggtagaattgtaatctattgaatttacgggccggatacgccacatccggcacaagcattaaggcaagaaaattattcgccgtc  <  2:79286‑M1/143‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAA  >  NC_000913/4049847‑4050131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.