Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,049,990 Δ12 bp noncoding (93‑104/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40499891 (0.020)47 (1.170) 42/282 0.0 97.9% noncoding (92/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 4050002 = NA (NA)noncoding (105/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

TTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTCC  >  NC_000913/4049862‑4050144
                                                                                                                                            |                                                                                                                                              
tacaaatatttttttctgaatttaacaaaaaagagtaaaggtagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatttt                                                                                                                                              <  2:502070‑M2/3‑1 (MQ=255)
tacaaaaagtgctttctgaactgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatttt                                                                                                                                              <  1:227316‑M2/3‑1 (MQ=255)
   aaaaagtgctttctgaactgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccattcagtaatttttct                                                                                                                                           >  1:541952‑M2/138‑141 (MQ=255)
    aaaagtgctttctgaactgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTAtt                                                                                                                                          <  1:359036‑M2/7‑1 (MQ=255)
       agtggtttctgaagcgaacaaaaaagaggaaagggagtcgcggagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTggg                                                                                                                                       <  2:150880‑M2/10‑1 (MQ=255)
               ctgaactgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGagaaagaa                                                                                                                               >  2:467310‑M2/126‑143 (MQ=255)
               ctgaactgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatATATATAGGGCgaaaaaa                                                                                                                               >  2:573614‑M2/126‑143 (MQ=255)
                 gaaccaaacaagaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGaaagaaaa                                                                                                                             <  2:226017‑M2/20‑1 (MQ=255)
                    ctgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGAt                                                                                                                          >  1:482517‑M2/121‑143 (MQ=255)
                     tgaacaaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGGATGAACAGATc                                                                                                                         >  2:673249‑M2/120‑143 (MQ=255)
                      gaacaaaaaagagttaagttagtcgcgtattttatagagttaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATcc                                                                                                                        <  2:272506‑M2/25‑1 (MQ=255)
                         caaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtactTTTTATTGGGCGAAAGAAAAGATCCGTa                                                                                                                     >  2:168109‑M2/116‑143 (MQ=255)
                         caaaaaagagtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattatagaagcaaaagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTa                                                                                                                     >  2:190281‑M2/116‑143 (MQ=255)
                                 agtaaagttagtcgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAAGGCCTGa                                                                                                             <  1:472596‑M2/36‑1 (MQ=255)
                                         tagtcgcgtagggtacagagggaagatgttctatcttttagggcttgtatttcacgtgagcggatttggctgggtattttggcggccgcagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTAt                                                                                                     >  2:705168‑M2/100‑143 (MQ=255)
                                             cgcgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGttt                                                                                                 <  1:460352‑M2/48‑1 (MQ=255)
                                               cgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTTTTGTGCGAAAGAAAAGATCCGTAATGCCTGATACGCTATGTTTAt                                                                                               >  2:121868‑M2/94‑143 (MQ=255)
                                               cgtagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatatattagccgccccagtcagtaatTTTTACTGGGCGAAAGAAAAGATCCGTAATGCGTGATTCGATATGTTTAt                                                                                               >  1:456488‑M2/94‑143 (MQ=255)
                                                 tagggtacagaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtattTTTTTTTTGTCTTATGAAAAGATCCATAATGCCTGATGCGCTATATTtataa                                                                                             >  2:55177‑M2/92‑141 (MQ=255)
                                                  ttgttacagtggttatatgttctatccttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAg                                                                                            <  2:452976‑M2/53‑1 (MQ=255)
                                                         agaggtaagatgttctatctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACg                                                                                     >  2:381979‑M2/84‑143 (MQ=255)
                                                               aattttttctatctttcatatcttgtacttcacggaatcggaattggctgaatagtttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGaa                                                                               <  2:563083‑M2/66‑1 (MQ=255)
                                                                        cTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTGAGTAAT‑‑‑TG‑‑‑‑‑‑‑‑‑TTATTGGGCGAAAGCAAAGATCCGTAATGCATGAACAAATATATTTATCAGACCAAAGGTAGAATTGTAATATATTGAAATTaaa                                                          >  2:651683/1‑141 (MQ=255)
                                                                          tctttcatacttttttcttgccgtatgcgggtgtggttgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTAt                                                                    <  1:722379‑M2/77‑1 (MQ=255)
                                                                          tctttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccctagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGTTATGTTTATCAGAGCAACGGTAGAattgtaaacgcg                                                                    >  1:270064‑M2/67‑138 (MQ=255)
                                                                             ttcagaccttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGTTGCGCTATGTTTATAAGGACAACGGTAGAATTGTAATCTATTGa                                                                 >  2:86413‑M2/64‑143 (MQ=255)
                                                                                  accttttccttctcgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTa                                                            <  1:409074‑M2/85‑1 (MQ=255)
                                                                                  accttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCGTCGGTCGAATTGTAATCTATTGAATTTa                                                            >  2:293679‑M2/59‑143 (MQ=255)
                                                                                   cctgttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTAc                                                           <  1:574678‑M2/86‑1 (MQ=255)
                                                                                    cttttacttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGCTGCGCTATGTTTATGAGGCCAACGGTAAAATTGTAATCTATTGAATTTaca                                                          >  2:581850‑M2/57‑142 (MQ=255)
                                                                                          cttcacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCgg                                                    >  1:559067‑M2/51‑143 (MQ=255)
                                                                                             cacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTTTGTTTATCAGGCCTACGGTAGATTTGTCATTTATAGAAATTACGAGCCGGATa                                                 >  2:240678‑M2/48‑143 (MQ=255)
                                                                                             cacgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATa                                                 >  1:396935‑M2/48‑143 (MQ=255)
                                                                                              acgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATAc                                                >  2:567099‑M2/47‑143 (MQ=255)
                                                                                               cgtaatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGCTGCGCTATGTTTATCCGGCCAACGGTAGAATTGTAATCTAATGAATTTACGGGCCGGATACg                                               >  2:425100‑M2/46‑143 (MQ=255)
                                                                                                ttaaccggatttgtctgaatattttagccgtcacagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGc                                              <  2:643214‑M2/99‑1 (MQ=255)
                                                                                                  aatcggatttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACCGACCGGATACGAca                                            >  1:364664‑M2/43‑143 (MQ=255)
                                                                                                      ggatttggctaaatatgttagccgccccattcagtaatTTTTATTGTGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTGTATCATGCCAACAGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATc                                        <  1:329866‑M2/105‑1 (MQ=255)
                                                                                                         tttggctgaatattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCgg                                     <  2:732089‑M2/108‑1 (MQ=255)
                                                                                                                 aatattttagccgccccagtcagtaatTTTTATTGGACGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGACCAACGGAAGAGTTGTAAACTATGAAATTTACGGGCCGGATACGCGACCACCGGcacaaaga                             >  1:551963‑M2/28‑140 (MQ=255)
                                                                                                                   tattttagccgccccagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTCTTGACTTTACGGGCCGGCTACGCCACATCCGGCACAAGCAtt                           >  2:148787‑M2/26‑143 (MQ=255)
                                                                                                                      tttagcctccccagtcagtgatTTTTCTTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAg                        <  1:259779‑M2/121‑1 (MQ=255)
                                                                                                                              ccccagtcagtaatTTTTATTAGGCGAAAGAAAAGATCTGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGAGCCGGATACGCCACATCAGGCACAAGCATTACGTCAAGaaa                >  1:85346‑M2/15‑143 (MQ=255)
                                                                                                                                 cagtcagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACCAGCCTTAAGGCAAGAACatt             >  2:633051‑M2/12‑143 (MQ=255)
                                                                                                                                     cagtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTc         >  1:21116‑M2/8‑143 (MQ=255)
                                                                                                                                      agtaatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTACTCTATTGAATTTACGGGCCGGATACGCAAAATCCGGCAAAAGCATTAAGGCAAGAaaattattaa        >  2:147462‑M2/7‑141 (MQ=255)
                                                                                                                                         aatTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCg     >  1:534334‑M2/4‑143 (MQ=255)
                                                                                                                                          atTTTTATTGGGCGCAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAACATTAAGGCAAGAAAATTATTCGCCGt    >  1:458666‑M2/3‑143 (MQ=255)
                                                                                                                                           tTTTTATTGGTAGATAGAAAAGATGCGTAATGCCGGATGCGCTGTGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTc   <  2:79286‑M2/142‑1 (MQ=255)
                                                                                                                                            ttttATTGGTCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTcc  <  1:565044/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
TTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTCC  >  NC_000913/4049862‑4050144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.