Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
MC JC | NC_000913 | 257,908 | Δ776 bp | [crl] | [crl] |
Missing coverage evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 257908–258675 | 258683 | 9–776 | 68 [0] | [0] 69 | [crl] | [crl] |
New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 257907 | 0 (0.000) | 63 (0.930) | 44/268 | 0.5 | 100% | intergenic (+8/‑769) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | 258684 = | 0 (0.000) | pseudogene (9/331 nt) | crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
GTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC > NC_000913/257767‑258049 | gtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag > 2:834515‑M1/1‑141 (MQ=255) gcaaaatttagcaaattttgccaatttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCAGTATACTCGTGAAGGTAaggg > 1:94584‑M1/13‑139 (MQ=255) caaaatttagcaaattttgccaattTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgc > 2:97378‑M1/12‑138 (MQ=255) aaaTTTGCTAACTTTTGCCAATTTGGCAAAAGAGTTGCATCACAACAGGACATAGCAATGACATTAACGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa < 1:523775‑M1/143‑8 (MQ=255) cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGAATATTCGTGAAGGTAagtgcaaagataat > 1:1034660‑M1/1‑129 (MQ=255) cTAAATTGTGCCAATGTGGTAGAACAGGTGCATCACAACAGGAGATAGCAATGACGGTACCGAGTGGACACCCGAAGAGCAGATTGAGCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat < 2:423049‑M1/143‑15 (MQ=255) tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc > 2:564034‑M1/1‑128 (MQ=255) aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTGACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcga < 2:318840‑M1/143‑18 (MQ=255) caatgaggtaaaacAGTTGCATCACACCAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt < 2:695134‑M1/137‑26 (MQ=255) cAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt < 2:1218368‑M1/143‑26 (MQ=255) aactttggacaacggttgcatcCCAACAGGAGATAGCCATGACGGTGCGGACTGGACACCCGAAGAGCAGATTGATCAACAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttc < 1:563948‑M1/133‑27 (MQ=255) tttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga > 2:1088547‑M1/1‑115 (MQ=255) aaaaCTGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTTGACGCCTGAAGAGCAGATTGATCAAAAAATTTACCGCACTGGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtct < 1:493301‑M1/143‑35 (MQ=255) aaaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagatactcgattctttttcgattgtct > 1:96739‑M1/1‑109 (MQ=255) aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTATGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttctgactgacga > 1:132359‑M1/1‑108 (MQ=255) aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATAAAAGAGTTTACCGCACTTGGCCCGTATTTTTGTGAAGGTAggtgcatcgtttatcgattctttttcgtttctctc > 2:23759‑M1/1‑108 (MQ=255) atcagttggcgcacgacaggagatagCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATGAAATAATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg < 1:552047‑M1/132‑37 (MQ=255) aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg > 1:348127‑M1/1‑107 (MQ=255) aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATGTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg > 2:1119122‑M1/1‑107 (MQ=255) ttGCATCAGAACAGAACATAGCAATCACGTTACCGAGCGGACACCTGAAGAGCAGATGGATCAAAATATTTACCTCACTAGGCTCGTATATTCGTGAAGGTAagcgcaaagataatcgattctttttcgattgtctggctgta < 2:202834‑M1/143‑42 (MQ=255) tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgt > 2:120364‑M1/1‑97 (MQ=255) tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtccggctgtatgcgt > 1:587822‑M1/1‑97 (MQ=255) caacaGGAGATGGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGGTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGCAagtgcaaagataatcgatactttttcgcttgtttcgctggatgcagcac > 2:566855‑M1/1‑94 (MQ=255) aacatgatatattgatgacgtgacctagtGGACACCCGAAGAGCAGAGTGAACAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac < 2:99410‑M1/129‑51 (MQ=255) caGGAGATAGCAATGACGTTACCGAGTGTACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt < 1:819514‑M1/143‑53 (MQ=255) gaagagagcaAGGCAGTTATCGAGTGGCCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga < 2:405008‑M1/141‑55 (MQ=255) gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGACGGTAagtgcaaagatcatcgattctttttcgattgtctggctgtatgcggcaacgtgaa > 2:316316‑M1/1‑88 (MQ=255) agaTAGCAATGACGTTACCGAGTGGATACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa < 2:337859‑M1/143‑57 (MQ=255) aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcacc < 2:442173‑M1/143‑65 (MQ=255) cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCGAAAAAGTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa < 2:846538‑M1/143‑69 (MQ=255) cGTTACCGAGCGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa < 1:1187187‑M1/143‑69 (MQ=255) tACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGGATATTCGTGAAGGTAagtgcaaagataatcgagtctttttcgattgtctggctgtatgcgtcaacgtgaaaccgccagcgaatcgg > 1:1071068‑M1/1‑72 (MQ=255) gAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcgtcaacgtgaaaccggaaacggaagagcgtg > 1:912066‑M1/1‑68 (MQ=255) gACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggacgtgcgtgcattc > 2:829888‑M1/1‑63 (MQ=255) cacCCGAAGAGCAGGTTGAGCAGAAACTGTACCGCGCTAGGCCCGTCGATTCGTGAAGGTAagtgcaaagagaagcggttcttttgcgagtgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg < 1:21385‑M1/143‑83 (MQ=255) cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCGCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg < 1:245232‑M1/143‑83 (MQ=255) cacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg < 1:1088547‑M1/143‑83 (MQ=255) cggatggtgatcaaaAAACTGACAGCACTAGGGCCGTTTAGTGGTGAAGGTAagtgcaaagataagcgattctctttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg < 1:1123384‑M1/136‑92 (MQ=255) gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgccaacgtgaaaccggcaccggaagtgcgtgaattctggggcgagcgg > 2:376389‑M1/1‑51 (MQ=255) cAGCTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggacgtgcgtgagttctcaggctcgtggg > 1:1236191‑M1/1‑50 (MQ=255) aGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat < 2:348127‑M1/143‑95 (MQ=255) gATTGATCACAAAATGGTCCGCACTAGGCCCGTGTATTGGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg < 2:168912‑M1/143‑96 (MQ=255) aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgacttcgggggctggtggatgg > 1:779682‑M1/1‑47 (MQ=255) aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggcgggtggatgg > 2:261280‑M1/1‑47 (MQ=255) aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGAGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgaataaccgtcaccggtagtgcgtagattctggggctggtggagcgcgct > 2:398275‑M1/1‑43 (MQ=255) tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcggttgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtggattctggggctggtggatggagctg > 2:313333‑M1/1‑42 (MQ=255) tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctg > 2:240284‑M1/1‑42 (MQ=255) aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggggtggtggatggagctgga > 1:360194‑M1/1‑40 (MQ=255) aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgga > 1:174448‑M1/1‑40 (MQ=255) tttCCCGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca < 2:1169199‑M1/143‑110 (MQ=255) aCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggttggtggatggagctggaagcgcagga > 2:631593‑M1/1‑31 (MQ=255) cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcagtgat > 1:393058‑M1/1‑29 (MQ=255) cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat < 1:476759‑M1/143‑115 (MQ=255) cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat > 2:150881‑M1/1‑29 (MQ=255) aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgtggctggtggatggagctggaagcgcaggaatccc < 1:97378‑M1/143‑118 (MQ=255) aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatccc < 2:938348‑M1/143‑118 (MQ=255) tAGGCGCGGATATTCGGGAAGGTAagtgcaaagagaagcgattctttggcgattggctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtggattctggggctggtggatggagctggaagcgcaggaatcccgt < 2:918433‑M1/143‑120 (MQ=255) cgggccctatactcgtggAGGGAagtgcgaagataatcgactcgtgtccgattgtctcgctgtatgcgtcaacgtgaaaccggcactggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtt < 2:728283‑M1/136‑121 (MQ=255) cccGTATATTCGTGAAGGTAagtgcaaaggtaatcgattctttttcgattgtctggctgtattcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttta < 2:630527‑M1/143‑124 (MQ=255) cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaccccgcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttta < 2:177278‑M1/143‑124 (MQ=255) agcgtttttgcgtggagggaagtgcaacgataatcgcttctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtccgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttta < 1:63611‑M1/133‑124 (MQ=255) ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacagccaccgggcgggcgtgaattctgcggctggtggctggagccggaagcgcgggaatcccgtgtcac > 1:1083831‑M1/1‑19 (MQ=255) atatTCGTGCAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctac < 2:923043‑M1/143‑129 (MQ=255) ggTGagtgcaaaggtaatcgattcttttttgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtt < 1:900096‑M1/143‑140 (MQ=255) gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttc > 1:260401‑M1/1‑3 (MQ=255) tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg < 1:120364‑M1/143‑142 (MQ=255) tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg < 2:671770‑M1/143‑142 (MQ=255) aagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccgccaccaaaacagcgtgaattctggggctggcgcatggagctggaagcgcaggaaacccgttttacctacagttgccagattgg > 1:44621‑M1/1‑1 (MQ=255) | GTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC > NC_000913/257767‑258049 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |